Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2202 and GFF2203 overlap by 4 nucleotides GFF2203 and GFF2204 are separated by 34 nucleotides GFF2204 and GFF2205 are separated by 146 nucleotides GFF2205 and GFF2206 overlap by 4 nucleotides GFF2206 and GFF2207 overlap by 4 nucleotides
GFF2202 - hypothetical protein, at 265,218 to 265,712
GFF2202
GFF2203 - no description, at 265,709 to 266,182
GFF2203
GFF2204 - no description, at 266,217 to 266,450
GFF2204
GFF2205 - hypothetical protein, at 266,597 to 267,580
GFF2205
GFF2206 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 267,577 to 268,539
GFF2206
GFF2207 - Flp pilus assembly protein TadB, at 268,536 to 269,534
GFF2207
Position (kb) 266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 265.603 kb on + strand, within GFF2202 at 265.790 kb on + strand, within GFF2203 at 265.985 kb on + strand, within GFF2203 at 265.990 kb on + strand, within GFF2203 at 265.999 kb on - strand, within GFF2203 at 266.071 kb on - strand, within GFF2203 at 266.311 kb on + strand, within GFF2204 at 266.311 kb on + strand, within GFF2204 at 266.312 kb on - strand, within GFF2204 at 266.425 kb on + strand, within GFF2204 at 266.606 kb on - strand at 266.937 kb on + strand, within GFF2205 at 267.120 kb on + strand, within GFF2205 at 267.196 kb on + strand, within GFF2205 at 267.197 kb on - strand, within GFF2205 at 267.197 kb on - strand, within GFF2205 at 267.198 kb on + strand, within GFF2205 at 267.198 kb on + strand, within GFF2205 at 267.199 kb on - strand, within GFF2205 at 267.199 kb on - strand, within GFF2205 at 267.360 kb on + strand, within GFF2205 at 267.360 kb on + strand, within GFF2205 at 267.360 kb on + strand, within GFF2205 at 267.563 kb on + strand at 267.564 kb on - strand at 267.887 kb on + strand, within GFF2206 at 267.922 kb on + strand, within GFF2206 at 267.923 kb on - strand, within GFF2206 at 267.923 kb on - strand, within GFF2206 at 268.560 kb on + strand at 268.560 kb on + strand at 268.560 kb on + strand at 268.560 kb on + strand at 268.560 kb on + strand at 268.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 265,603 + GFF2202 0.78 +0.7 265,790 + GFF2203 0.17 -0.6 265,985 + GFF2203 0.58 +0.6 265,990 + GFF2203 0.59 -1.0 265,999 - GFF2203 0.61 -0.4 266,071 - GFF2203 0.76 +1.2 266,311 + GFF2204 0.40 +1.2 266,311 + GFF2204 0.40 -0.0 266,312 - GFF2204 0.41 +0.7 266,425 + GFF2204 0.89 +1.3 266,606 - -1.2 266,937 + GFF2205 0.35 +0.6 267,120 + GFF2205 0.53 +1.0 267,196 + GFF2205 0.61 -3.5 267,197 - GFF2205 0.61 +0.7 267,197 - GFF2205 0.61 -0.0 267,198 + GFF2205 0.61 +0.3 267,198 + GFF2205 0.61 +0.9 267,199 - GFF2205 0.61 +0.8 267,199 - GFF2205 0.61 -0.2 267,360 + GFF2205 0.78 -0.5 267,360 + GFF2205 0.78 +0.8 267,360 + GFF2205 0.78 +0.7 267,563 + +0.7 267,564 - -1.9 267,887 + GFF2206 0.32 -0.2 267,922 + GFF2206 0.36 +0.3 267,923 - GFF2206 0.36 -0.0 267,923 - GFF2206 0.36 +2.3 268,560 + +0.2 268,560 + +0.1 268,560 + -1.0 268,560 + -0.0 268,560 + +0.6 268,561 - +0.8
Or see this region's nucleotide sequence