Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1769 and GFF1770 are separated by 90 nucleotides GFF1770 and GFF1771 overlap by 4 nucleotides GFF1771 and GFF1772 are separated by 292 nucleotides
GFF1769 - Urease accessory protein UreD, at 431,432 to 432,262
GFF1769
GFF1770 - cytidine/deoxycytidylate deaminase family protein, at 432,353 to 432,880
GFF1770
GFF1771 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 432,877 to 433,914
GFF1771
GFF1772 - Putative Rieske (2Fe-2S) family oxidoreductase, large subunit, at 434,207 to 435,289
GFF1772
Position (kb)
432
433
434 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 431.926 kb on - strand, within GFF1769 at 431.926 kb on - strand, within GFF1769 at 431.926 kb on - strand, within GFF1769 at 431.926 kb on - strand, within GFF1769 at 432.175 kb on + strand, within GFF1769 at 432.176 kb on - strand, within GFF1769 at 432.231 kb on + strand at 432.232 kb on - strand at 432.232 kb on - strand at 432.232 kb on - strand at 432.273 kb on + strand at 432.273 kb on + strand at 432.273 kb on + strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.274 kb on - strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.276 kb on + strand at 432.277 kb on - strand at 432.277 kb on - strand at 432.277 kb on - strand at 432.354 kb on + strand at 432.355 kb on - strand at 432.355 kb on - strand at 432.355 kb on - strand at 432.591 kb on + strand, within GFF1770 at 432.591 kb on + strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.592 kb on - strand, within GFF1770 at 432.846 kb on + strand at 432.846 kb on + strand at 433.012 kb on + strand, within GFF1771 at 433.012 kb on + strand, within GFF1771 at 433.014 kb on + strand, within GFF1771 at 433.015 kb on - strand, within GFF1771 at 433.015 kb on - strand, within GFF1771 at 433.055 kb on + strand, within GFF1771 at 433.055 kb on + strand, within GFF1771 at 433.055 kb on + strand, within GFF1771 at 433.056 kb on - strand, within GFF1771 at 433.274 kb on + strand, within GFF1771 at 433.274 kb on + strand, within GFF1771 at 433.341 kb on - strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.571 kb on + strand, within GFF1771 at 433.572 kb on - strand, within GFF1771 at 433.572 kb on - strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.574 kb on + strand, within GFF1771 at 433.575 kb on - strand, within GFF1771 at 433.575 kb on - strand, within GFF1771 at 433.580 kb on + strand, within GFF1771 at 433.661 kb on + strand, within GFF1771 at 433.662 kb on - strand, within GFF1771 at 433.713 kb on - strand, within GFF1771 at 433.742 kb on + strand, within GFF1771 at 433.742 kb on + strand, within GFF1771 at 433.742 kb on + strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.743 kb on - strand, within GFF1771 at 433.746 kb on - strand, within GFF1771 at 433.746 kb on - strand, within GFF1771 at 433.746 kb on - strand, within GFF1771 at 434.028 kb on + strand at 434.028 kb on + strand at 434.028 kb on + strand at 434.028 kb on + strand at 434.029 kb on - strand at 434.029 kb on - strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.116 kb on + strand at 434.117 kb on - strand at 434.117 kb on - strand at 434.117 kb on - strand at 434.465 kb on + strand, within GFF1772 at 434.466 kb on - strand, within GFF1772 at 434.466 kb on - strand, within GFF1772 at 434.491 kb on + strand, within GFF1772 at 434.491 kb on + strand, within GFF1772 at 434.502 kb on - strand, within GFF1772 at 434.546 kb on + strand, within GFF1772 at 434.546 kb on + strand, within GFF1772 at 434.547 kb on - strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.570 kb on + strand, within GFF1772 at 434.571 kb on - strand, within GFF1772 at 434.571 kb on - strand, within GFF1772 at 434.571 kb on - strand, within GFF1772 at 434.660 kb on + strand, within GFF1772 at 434.660 kb on + strand, within GFF1772 at 434.777 kb on + strand, within GFF1772 at 434.777 kb on + strand, within GFF1772 at 434.777 kb on + strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.778 kb on - strand, within GFF1772 at 434.903 kb on + strand, within GFF1772 at 434.903 kb on + strand, within GFF1772 at 434.903 kb on + strand, within GFF1772 at 434.903 kb on + strand, within GFF1772
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 431,926 - GFF1769 0.59 -0.1 431,926 - GFF1769 0.59 -0.8 431,926 - GFF1769 0.59 -0.1 431,926 - GFF1769 0.59 -0.5 432,175 + GFF1769 0.89 +0.2 432,176 - GFF1769 0.90 +0.8 432,231 + +0.7 432,232 - -0.1 432,232 - -0.7 432,232 - +0.1 432,273 + -0.7 432,273 + -1.8 432,273 + -0.0 432,274 - -0.0 432,274 - -0.3 432,274 - -0.0 432,274 - -0.5 432,274 - -0.6 432,276 + -0.7 432,276 + -0.4 432,276 + -0.6 432,276 + -0.5 432,276 + -0.6 432,276 + +0.2 432,277 - -0.2 432,277 - -0.9 432,277 - -1.1 432,354 + -0.6 432,355 - +0.3 432,355 - -0.6 432,355 - -0.1 432,591 + GFF1770 0.45 +0.6 432,591 + GFF1770 0.45 -0.3 432,592 - GFF1770 0.45 -0.1 432,592 - GFF1770 0.45 -0.0 432,592 - GFF1770 0.45 +0.2 432,592 - GFF1770 0.45 +0.1 432,592 - GFF1770 0.45 +0.2 432,846 + +0.7 432,846 + +0.2 433,012 + GFF1771 0.13 -0.0 433,012 + GFF1771 0.13 -0.0 433,014 + GFF1771 0.13 +0.1 433,015 - GFF1771 0.13 -1.5 433,015 - GFF1771 0.13 +0.7 433,055 + GFF1771 0.17 -1.0 433,055 + GFF1771 0.17 -1.0 433,055 + GFF1771 0.17 +1.4 433,056 - GFF1771 0.17 -0.4 433,274 + GFF1771 0.38 -0.2 433,274 + GFF1771 0.38 -0.3 433,341 - GFF1771 0.45 -0.0 433,571 + GFF1771 0.67 -0.7 433,571 + GFF1771 0.67 +1.7 433,571 + GFF1771 0.67 -1.6 433,571 + GFF1771 0.67 -0.3 433,572 - GFF1771 0.67 -1.3 433,572 - GFF1771 0.67 +1.1 433,574 + GFF1771 0.67 +1.2 433,574 + GFF1771 0.67 -1.5 433,574 + GFF1771 0.67 -0.6 433,574 + GFF1771 0.67 -1.2 433,574 + GFF1771 0.67 -1.9 433,575 - GFF1771 0.67 -0.8 433,575 - GFF1771 0.67 -1.1 433,580 + GFF1771 0.68 +0.4 433,661 + GFF1771 0.76 +0.5 433,662 - GFF1771 0.76 +0.7 433,713 - GFF1771 0.81 -0.9 433,742 + GFF1771 0.83 -0.3 433,742 + GFF1771 0.83 -0.6 433,742 + GFF1771 0.83 -0.8 433,743 - GFF1771 0.83 -1.6 433,743 - GFF1771 0.83 -1.3 433,743 - GFF1771 0.83 -0.1 433,743 - GFF1771 0.83 -0.3 433,746 - GFF1771 0.84 -1.1 433,746 - GFF1771 0.84 -1.5 433,746 - GFF1771 0.84 +2.4 434,028 + +0.2 434,028 + +0.9 434,028 + -0.2 434,028 + -0.2 434,029 - -0.4 434,029 - +0.0 434,116 + +0.4 434,116 + +0.1 434,116 + +0.4 434,116 + +1.7 434,116 + +1.0 434,117 - +0.8 434,117 - -0.3 434,117 - +0.3 434,465 + GFF1772 0.24 -0.1 434,466 - GFF1772 0.24 +0.1 434,466 - GFF1772 0.24 +0.5 434,491 + GFF1772 0.26 -1.0 434,491 + GFF1772 0.26 +0.4 434,502 - GFF1772 0.27 +0.5 434,546 + GFF1772 0.31 +2.7 434,546 + GFF1772 0.31 -1.4 434,547 - GFF1772 0.31 -1.3 434,570 + GFF1772 0.34 -0.8 434,570 + GFF1772 0.34 +0.8 434,570 + GFF1772 0.34 -0.1 434,570 + GFF1772 0.34 -1.3 434,571 - GFF1772 0.34 +0.4 434,571 - GFF1772 0.34 -0.1 434,571 - GFF1772 0.34 -0.3 434,660 + GFF1772 0.42 -0.4 434,660 + GFF1772 0.42 +0.4 434,777 + GFF1772 0.53 +0.3 434,777 + GFF1772 0.53 -0.4 434,777 + GFF1772 0.53 -0.4 434,778 - GFF1772 0.53 +0.0 434,778 - GFF1772 0.53 -0.8 434,778 - GFF1772 0.53 -0.1 434,778 - GFF1772 0.53 +1.7 434,778 - GFF1772 0.53 -0.3 434,778 - GFF1772 0.53 +0.1 434,778 - GFF1772 0.53 -0.3 434,903 + GFF1772 0.64 -1.1 434,903 + GFF1772 0.64 +0.2 434,903 + GFF1772 0.64 -0.8 434,903 + GFF1772 0.64 -0.0
Or see this region's nucleotide sequence