Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF174 and GFF175 are separated by 36 nucleotides GFF175 and GFF176 overlap by 1 nucleotides GFF176 and GFF177 overlap by 4 nucleotides GFF177 and GFF178 are separated by 39 nucleotides
GFF174 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 205,428 to 206,258
GFF174
GFF175 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 206,295 to 206,960
GFF175
GFF176 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 206,960 to 207,619
GFF176
GFF177 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 207,616 to 208,386
GFF177
GFF178 - Mannonate dehydratase (EC 4.2.1.8), at 208,426 to 209,637
GFF178
Position (kb)
206
207
208 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 206.176 kb on - strand at 206.176 kb on - strand at 206.334 kb on + strand at 206.335 kb on - strand at 206.335 kb on - strand at 206.430 kb on + strand, within GFF175 at 206.456 kb on + strand, within GFF175 at 206.456 kb on + strand, within GFF175 at 206.457 kb on - strand, within GFF175 at 206.457 kb on - strand, within GFF175 at 206.457 kb on - strand, within GFF175 at 206.466 kb on + strand, within GFF175 at 206.466 kb on + strand, within GFF175 at 206.466 kb on + strand, within GFF175 at 206.466 kb on + strand, within GFF175 at 206.467 kb on - strand, within GFF175 at 206.467 kb on - strand, within GFF175 at 206.467 kb on - strand, within GFF175 at 206.467 kb on - strand, within GFF175 at 206.517 kb on + strand, within GFF175 at 206.517 kb on + strand, within GFF175 at 206.518 kb on - strand, within GFF175 at 206.518 kb on - strand, within GFF175 at 206.601 kb on + strand, within GFF175 at 206.601 kb on + strand, within GFF175 at 206.601 kb on + strand, within GFF175 at 206.602 kb on - strand, within GFF175 at 206.602 kb on - strand, within GFF175 at 206.602 kb on - strand, within GFF175 at 206.602 kb on - strand, within GFF175 at 206.844 kb on + strand, within GFF175 at 206.883 kb on + strand, within GFF175 at 206.883 kb on + strand, within GFF175 at 206.883 kb on + strand, within GFF175 at 206.883 kb on + strand, within GFF175 at 206.883 kb on + strand, within GFF175 at 206.884 kb on - strand, within GFF175 at 206.884 kb on - strand, within GFF175 at 206.884 kb on - strand, within GFF175 at 206.884 kb on - strand, within GFF175 at 206.884 kb on - strand, within GFF175 at 206.884 kb on - strand, within GFF175 at 206.884 kb on - strand, within GFF175 at 206.928 kb on + strand at 206.928 kb on + strand at 206.928 kb on + strand at 206.929 kb on - strand at 206.929 kb on - strand at 206.929 kb on - strand at 206.929 kb on - strand at 206.929 kb on - strand at 206.996 kb on + strand at 206.997 kb on - strand at 207.173 kb on + strand, within GFF176 at 207.173 kb on + strand, within GFF176 at 207.174 kb on - strand, within GFF176 at 207.185 kb on + strand, within GFF176 at 207.186 kb on - strand, within GFF176 at 207.186 kb on - strand, within GFF176 at 207.186 kb on - strand, within GFF176 at 207.186 kb on - strand, within GFF176 at 207.266 kb on + strand, within GFF176 at 207.267 kb on - strand, within GFF176 at 207.267 kb on - strand, within GFF176 at 207.548 kb on + strand, within GFF176 at 207.548 kb on + strand, within GFF176 at 207.549 kb on - strand, within GFF176 at 207.549 kb on - strand, within GFF176 at 207.591 kb on + strand at 207.591 kb on + strand at 207.591 kb on + strand at 207.664 kb on + strand at 207.664 kb on + strand at 207.665 kb on - strand at 207.665 kb on - strand at 207.665 kb on - strand at 207.665 kb on - strand at 207.685 kb on + strand at 207.685 kb on + strand at 207.685 kb on + strand at 207.685 kb on + strand at 207.686 kb on - strand at 208.170 kb on - strand, within GFF177 at 208.170 kb on - strand, within GFF177 at 208.418 kb on + strand at 208.418 kb on + strand at 208.418 kb on + strand at 208.418 kb on + strand at 208.419 kb on - strand at 208.562 kb on - strand, within GFF178
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 206,176 - +0.8 206,176 - -0.3 206,334 + -1.8 206,335 - +1.9 206,335 - +0.9 206,430 + GFF175 0.20 -0.6 206,456 + GFF175 0.24 -1.4 206,456 + GFF175 0.24 -0.3 206,457 - GFF175 0.24 -0.2 206,457 - GFF175 0.24 -0.7 206,457 - GFF175 0.24 -0.3 206,466 + GFF175 0.26 +0.2 206,466 + GFF175 0.26 -1.1 206,466 + GFF175 0.26 +0.6 206,466 + GFF175 0.26 -1.4 206,467 - GFF175 0.26 +0.2 206,467 - GFF175 0.26 +1.2 206,467 - GFF175 0.26 -2.3 206,467 - GFF175 0.26 +0.4 206,517 + GFF175 0.33 -1.1 206,517 + GFF175 0.33 -1.4 206,518 - GFF175 0.33 -0.2 206,518 - GFF175 0.33 +1.5 206,601 + GFF175 0.46 -0.5 206,601 + GFF175 0.46 -0.4 206,601 + GFF175 0.46 -0.4 206,602 - GFF175 0.46 +0.2 206,602 - GFF175 0.46 -1.4 206,602 - GFF175 0.46 -1.2 206,602 - GFF175 0.46 -0.4 206,844 + GFF175 0.82 +0.1 206,883 + GFF175 0.88 -0.0 206,883 + GFF175 0.88 +0.4 206,883 + GFF175 0.88 -0.9 206,883 + GFF175 0.88 +0.2 206,883 + GFF175 0.88 +0.2 206,884 - GFF175 0.88 +0.5 206,884 - GFF175 0.88 +0.1 206,884 - GFF175 0.88 -0.6 206,884 - GFF175 0.88 +0.4 206,884 - GFF175 0.88 -0.4 206,884 - GFF175 0.88 -0.4 206,884 - GFF175 0.88 -0.4 206,928 + +0.9 206,928 + +0.2 206,928 + -0.7 206,929 - +0.2 206,929 - -0.5 206,929 - -0.8 206,929 - +0.5 206,929 - -0.1 206,996 + -1.8 206,997 - -0.4 207,173 + GFF176 0.32 -0.4 207,173 + GFF176 0.32 -1.2 207,174 - GFF176 0.32 +0.4 207,185 + GFF176 0.34 -0.7 207,186 - GFF176 0.34 +0.5 207,186 - GFF176 0.34 +0.8 207,186 - GFF176 0.34 -0.9 207,186 - GFF176 0.34 -0.1 207,266 + GFF176 0.46 -1.5 207,267 - GFF176 0.47 -1.6 207,267 - GFF176 0.47 -0.4 207,548 + GFF176 0.89 +0.6 207,548 + GFF176 0.89 -0.7 207,549 - GFF176 0.89 -0.9 207,549 - GFF176 0.89 -1.3 207,591 + -0.1 207,591 + +0.6 207,591 + -0.0 207,664 + +0.6 207,664 + -1.6 207,665 - +0.1 207,665 - -0.3 207,665 - +0.3 207,665 - -0.2 207,685 + +2.2 207,685 + -0.5 207,685 + -0.6 207,685 + -1.7 207,686 - -2.4 208,170 - GFF177 0.72 -0.0 208,170 - GFF177 0.72 +1.0 208,418 + -0.5 208,418 + +0.6 208,418 + +1.9 208,418 + -0.2 208,419 - +0.2 208,562 - GFF178 0.11 -0.3
Or see this region's nucleotide sequence