Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1671 and GFF1672 overlap by 4 nucleotides GFF1672 and GFF1673 are separated by 30 nucleotides GFF1673 and GFF1674 overlap by 4 nucleotides GFF1674 and GFF1675 overlap by 8 nucleotides
GFF1671 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 316,589 to 317,863
GFF1671
GFF1672 - BUG/TctC family periplasmic protein, at 317,860 to 318,825
GFF1672
GFF1673 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 318,856 to 319,566
GFF1673
GFF1674 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 319,563 to 320,324
GFF1674
GFF1675 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 320,317 to 321,297
GFF1675
Position (kb) 318
319
320 Strain fitness (log2 ratio) -1
0
1 at 317.879 kb on + strand at 318.279 kb on - strand, within GFF1672 at 318.279 kb on - strand, within GFF1672 at 318.279 kb on - strand, within GFF1672 at 318.315 kb on - strand, within GFF1672 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 318.947 kb on + strand, within GFF1673 at 319.203 kb on + strand, within GFF1673 at 319.621 kb on + strand at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675 at 320.423 kb on + strand, within GFF1675
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 317,879 + +0.0 318,279 - GFF1672 0.43 -0.3 318,279 - GFF1672 0.43 -0.3 318,279 - GFF1672 0.43 -1.2 318,315 - GFF1672 0.47 -0.8 318,947 + GFF1673 0.13 -0.4 318,947 + GFF1673 0.13 +0.5 318,947 + GFF1673 0.13 -0.7 319,203 + GFF1673 0.49 -0.8 319,621 + -0.9 320,423 + GFF1675 0.11 -0.4 320,423 + GFF1675 0.11 -0.6 320,423 + GFF1675 0.11 -0.8 320,423 + GFF1675 0.11 -0.8 320,423 + GFF1675 0.11 +0.0
Or see this region's nucleotide sequence