Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1601 and GFF1602 are separated by 23 nucleotides GFF1602 and GFF1603 overlap by 4 nucleotides GFF1603 and GFF1604 are separated by 1 nucleotides
GFF1601 - no description, at 199,020 to 200,219
GFF1601
GFF1602 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 200,243 to 200,965
GFF1602
GFF1603 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 200,962 to 201,729
GFF1603
GFF1604 - Prolidase (EC 3.4.13.9), at 201,731 to 202,996
GFF1604
Position (kb) 200
201
202 Strain fitness (log2 ratio) -2
-1
0
1 at 200.213 kb on + strand at 200.652 kb on + strand, within GFF1602 at 200.652 kb on + strand, within GFF1602 at 200.985 kb on - strand at 200.985 kb on - strand at 201.042 kb on - strand, within GFF1603 at 201.758 kb on + strand at 202.302 kb on + strand, within GFF1604 at 202.303 kb on - strand, within GFF1604 at 202.303 kb on - strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.432 kb on + strand, within GFF1604 at 202.433 kb on - strand, within GFF1604 at 202.433 kb on - strand, within GFF1604
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 200,213 + -0.1 200,652 + GFF1602 0.57 -0.1 200,652 + GFF1602 0.57 +1.5 200,985 - +1.0 200,985 - -0.9 201,042 - GFF1603 0.10 -1.8 201,758 + -0.9 202,302 + GFF1604 0.45 +0.4 202,303 - GFF1604 0.45 +0.7 202,303 - GFF1604 0.45 -0.1 202,320 + GFF1604 0.47 +0.5 202,320 + GFF1604 0.47 -0.1 202,320 + GFF1604 0.47 -1.0 202,321 - GFF1604 0.47 -1.4 202,321 - GFF1604 0.47 +0.7 202,321 - GFF1604 0.47 +1.3 202,321 - GFF1604 0.47 +0.8 202,432 + GFF1604 0.55 +0.3 202,433 - GFF1604 0.55 -0.2 202,433 - GFF1604 0.55 -0.4
Or see this region's nucleotide sequence