Strain Fitness in Variovorax sp. SCN45 around GFF155

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF154 and GFF155 are separated by 33 nucleotidesGFF155 and GFF156 are separated by 16 nucleotidesGFF156 and GFF157 overlap by 4 nucleotides GFF154 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 181,508 to 183,178 GFF154 GFF155 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 183,212 to 184,783 GFF155 GFF156 - Glycosyl transferase, group 2 family, at 184,800 to 185,498 GFF156 GFF157 - Methyltransferase type 12, at 185,495 to 186,100 GFF157 Position (kb) 183 184 185Strain fitness (log2 ratio) -2 -1 0 1 2at 182.273 kb on + strand, within GFF154at 182.273 kb on + strand, within GFF154at 182.273 kb on + strand, within GFF154at 182.373 kb on - strand, within GFF154at 182.373 kb on - strand, within GFF154at 182.373 kb on - strand, within GFF154at 182.630 kb on + strand, within GFF154at 182.630 kb on + strand, within GFF154at 182.630 kb on + strand, within GFF154at 182.631 kb on - strand, within GFF154at 182.631 kb on - strand, within GFF154at 182.777 kb on + strand, within GFF154at 182.777 kb on + strand, within GFF154at 182.778 kb on - strand, within GFF154at 183.075 kb on - strandat 183.444 kb on + strand, within GFF155at 183.445 kb on - strand, within GFF155at 183.464 kb on + strand, within GFF155at 183.464 kb on + strand, within GFF155at 183.464 kb on + strand, within GFF155at 183.464 kb on + strand, within GFF155at 183.465 kb on - strand, within GFF155at 183.465 kb on - strand, within GFF155at 183.548 kb on + strand, within GFF155at 183.548 kb on + strand, within GFF155at 183.548 kb on + strand, within GFF155at 183.549 kb on - strand, within GFF155at 183.549 kb on - strand, within GFF155at 183.549 kb on - strand, within GFF155at 183.549 kb on - strand, within GFF155at 183.549 kb on - strand, within GFF155at 183.587 kb on + strand, within GFF155at 183.812 kb on + strand, within GFF155at 183.812 kb on + strand, within GFF155at 183.812 kb on + strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.813 kb on - strand, within GFF155at 183.839 kb on + strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.840 kb on - strand, within GFF155at 183.875 kb on + strand, within GFF155at 183.875 kb on + strand, within GFF155at 183.875 kb on + strand, within GFF155at 183.876 kb on - strand, within GFF155at 183.876 kb on - strand, within GFF155at 184.215 kb on - strand, within GFF155at 184.215 kb on - strand, within GFF155at 184.244 kb on + strand, within GFF155at 184.244 kb on + strand, within GFF155at 184.244 kb on + strand, within GFF155at 184.245 kb on - strand, within GFF155at 184.286 kb on + strand, within GFF155at 184.286 kb on + strand, within GFF155at 184.287 kb on - strand, within GFF155at 184.287 kb on - strand, within GFF155at 184.340 kb on + strand, within GFF155at 184.340 kb on + strand, within GFF155at 184.340 kb on + strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.341 kb on - strand, within GFF155at 184.433 kb on + strand, within GFF155at 184.434 kb on - strand, within GFF155at 184.434 kb on - strand, within GFF155at 184.448 kb on + strand, within GFF155at 184.448 kb on + strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.449 kb on - strand, within GFF155at 184.469 kb on + strand, within GFF155at 184.470 kb on - strand, within GFF155at 184.470 kb on - strand, within GFF155at 184.499 kb on + strand, within GFF155at 184.500 kb on - strand, within GFF155at 184.500 kb on - strand, within GFF155at 184.657 kb on + strandat 184.657 kb on + strandat 184.657 kb on + strandat 184.657 kb on + strandat 184.657 kb on + strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.658 kb on - strandat 184.907 kb on + strand, within GFF156at 184.908 kb on - strand, within GFF156at 184.908 kb on - strand, within GFF156at 185.083 kb on + strand, within GFF156at 185.083 kb on + strand, within GFF156at 185.084 kb on - strand, within GFF156at 185.084 kb on - strand, within GFF156at 185.746 kb on - strand, within GFF157

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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182,273 + GFF154 0.46 -0.8
182,273 + GFF154 0.46 -0.7
182,273 + GFF154 0.46 -0.8
182,373 - GFF154 0.52 -1.1
182,373 - GFF154 0.52 -0.0
182,373 - GFF154 0.52 +0.4
182,630 + GFF154 0.67 +0.2
182,630 + GFF154 0.67 -0.5
182,630 + GFF154 0.67 +0.6
182,631 - GFF154 0.67 -0.0
182,631 - GFF154 0.67 -0.4
182,777 + GFF154 0.76 -0.5
182,777 + GFF154 0.76 -0.1
182,778 - GFF154 0.76 -0.5
183,075 - -0.3
183,444 + GFF155 0.15 -0.5
183,445 - GFF155 0.15 -1.1
183,464 + GFF155 0.16 +0.6
183,464 + GFF155 0.16 +0.7
183,464 + GFF155 0.16 -0.8
183,464 + GFF155 0.16 +1.6
183,465 - GFF155 0.16 +0.7
183,465 - GFF155 0.16 -0.4
183,548 + GFF155 0.21 +0.2
183,548 + GFF155 0.21 -0.7
183,548 + GFF155 0.21 +0.4
183,549 - GFF155 0.21 -1.4
183,549 - GFF155 0.21 -0.8
183,549 - GFF155 0.21 -1.8
183,549 - GFF155 0.21 -1.2
183,549 - GFF155 0.21 -0.6
183,587 + GFF155 0.24 +0.7
183,812 + GFF155 0.38 -0.5
183,812 + GFF155 0.38 -0.5
183,812 + GFF155 0.38 +1.1
183,813 - GFF155 0.38 -0.3
183,813 - GFF155 0.38 +0.8
183,813 - GFF155 0.38 +0.3
183,813 - GFF155 0.38 -0.2
183,813 - GFF155 0.38 -0.4
183,839 + GFF155 0.40 -0.4
183,840 - GFF155 0.40 -0.2
183,840 - GFF155 0.40 +0.6
183,840 - GFF155 0.40 +0.2
183,840 - GFF155 0.40 +0.5
183,840 - GFF155 0.40 -0.5
183,875 + GFF155 0.42 +0.5
183,875 + GFF155 0.42 -0.7
183,875 + GFF155 0.42 -2.3
183,876 - GFF155 0.42 +1.5
183,876 - GFF155 0.42 -0.2
184,215 - GFF155 0.64 +0.0
184,215 - GFF155 0.64 -0.0
184,244 + GFF155 0.66 -0.1
184,244 + GFF155 0.66 -0.8
184,244 + GFF155 0.66 -0.3
184,245 - GFF155 0.66 -0.1
184,286 + GFF155 0.68 +0.9
184,286 + GFF155 0.68 -0.2
184,287 - GFF155 0.68 -0.6
184,287 - GFF155 0.68 -0.5
184,340 + GFF155 0.72 +0.9
184,340 + GFF155 0.72 -0.2
184,340 + GFF155 0.72 -1.0
184,341 - GFF155 0.72 -0.4
184,341 - GFF155 0.72 -0.8
184,341 - GFF155 0.72 -1.4
184,341 - GFF155 0.72 +0.6
184,433 + GFF155 0.78 -1.0
184,434 - GFF155 0.78 -0.8
184,434 - GFF155 0.78 -0.1
184,448 + GFF155 0.79 +0.5
184,448 + GFF155 0.79 -0.4
184,449 - GFF155 0.79 +1.0
184,449 - GFF155 0.79 +0.9
184,449 - GFF155 0.79 -0.1
184,449 - GFF155 0.79 +0.8
184,469 + GFF155 0.80 -0.7
184,470 - GFF155 0.80 +1.0
184,470 - GFF155 0.80 -0.4
184,499 + GFF155 0.82 +0.2
184,500 - GFF155 0.82 -2.0
184,500 - GFF155 0.82 +0.1
184,657 + -0.4
184,657 + +0.5
184,657 + -2.1
184,657 + -0.6
184,657 + +0.7
184,658 - -0.4
184,658 - -0.1
184,658 - +1.3
184,658 - +0.1
184,658 - -1.0
184,907 + GFF156 0.15 +1.2
184,908 - GFF156 0.15 -0.0
184,908 - GFF156 0.15 -0.2
185,083 + GFF156 0.40 +2.5
185,083 + GFF156 0.40 -0.4
185,084 - GFF156 0.41 +0.2
185,084 - GFF156 0.41 +0.2
185,746 - GFF157 0.41 +0.8

Or see this region's nucleotide sequence