Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1450 and GFF1451 are separated by 5 nucleotides GFF1451 and GFF1452 are separated by 43 nucleotides GFF1452 and GFF1453 are separated by 6 nucleotides
GFF1450 - similar to ribulose-1,5-bisphosphate carboxylase, Type III, at 42,046 to 43,314
GFF1450
GFF1451 - FIG00456379: hypothetical protein, at 43,320 to 43,859
GFF1451
GFF1452 - Predicted gluconate TRAP family transporter, DctM subunit, at 43,903 to 45,792
GFF1452
GFF1453 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 45,799 to 46,818
GFF1453
Position (kb) 43
44
45
46 Strain fitness (log2 ratio) -2
-1
0
1
2 at 43.286 kb on + strand at 43.457 kb on - strand, within GFF1451 at 43.556 kb on - strand, within GFF1451 at 43.571 kb on - strand, within GFF1451 at 43.667 kb on - strand, within GFF1451 at 43.753 kb on + strand, within GFF1451 at 43.754 kb on - strand, within GFF1451 at 43.754 kb on - strand, within GFF1451 at 43.754 kb on - strand, within GFF1451 at 43.789 kb on + strand, within GFF1451 at 43.789 kb on + strand, within GFF1451 at 43.822 kb on + strand at 43.823 kb on - strand at 43.823 kb on - strand at 43.823 kb on - strand at 43.823 kb on - strand at 43.823 kb on - strand at 43.980 kb on - strand at 44.042 kb on + strand at 44.042 kb on + strand at 44.043 kb on - strand at 44.106 kb on - strand, within GFF1452 at 44.237 kb on + strand, within GFF1452 at 44.237 kb on + strand, within GFF1452 at 44.238 kb on - strand, within GFF1452 at 44.238 kb on - strand, within GFF1452 at 44.564 kb on + strand, within GFF1452 at 44.565 kb on - strand, within GFF1452 at 44.565 kb on - strand, within GFF1452 at 44.870 kb on + strand, within GFF1452 at 44.871 kb on - strand, within GFF1452 at 44.891 kb on + strand, within GFF1452 at 44.891 kb on + strand, within GFF1452 at 44.891 kb on + strand, within GFF1452 at 45.598 kb on + strand, within GFF1452 at 45.598 kb on + strand, within GFF1452 at 45.599 kb on - strand, within GFF1452 at 45.599 kb on - strand, within GFF1452 at 45.599 kb on - strand, within GFF1452 at 45.620 kb on + strand at 45.872 kb on + strand at 46.205 kb on + strand, within GFF1453 at 46.205 kb on + strand, within GFF1453 at 46.205 kb on + strand, within GFF1453 at 46.205 kb on + strand, within GFF1453 at 46.206 kb on - strand, within GFF1453 at 46.206 kb on - strand, within GFF1453 at 46.206 kb on - strand, within GFF1453 at 46.382 kb on + strand, within GFF1453 at 46.382 kb on + strand, within GFF1453 at 46.383 kb on - strand, within GFF1453 at 46.383 kb on - strand, within GFF1453 at 46.457 kb on + strand, within GFF1453 at 46.457 kb on + strand, within GFF1453 at 46.457 kb on + strand, within GFF1453 at 46.458 kb on - strand, within GFF1453 at 46.458 kb on - strand, within GFF1453 at 46.458 kb on - strand, within GFF1453 at 46.458 kb on - strand, within GFF1453 at 46.458 kb on - strand, within GFF1453 at 46.706 kb on + strand, within GFF1453 at 46.706 kb on + strand, within GFF1453 at 46.706 kb on + strand, within GFF1453 at 46.707 kb on - strand, within GFF1453 at 46.707 kb on - strand, within GFF1453 at 46.707 kb on - strand, within GFF1453 at 46.707 kb on - strand, within GFF1453 at 46.712 kb on + strand, within GFF1453 at 46.713 kb on - strand, within GFF1453 at 46.713 kb on - strand, within GFF1453 at 46.713 kb on - strand, within GFF1453
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 43,286 + -0.1 43,457 - GFF1451 0.25 -0.5 43,556 - GFF1451 0.44 +1.2 43,571 - GFF1451 0.46 -1.5 43,667 - GFF1451 0.64 +0.2 43,753 + GFF1451 0.80 +1.5 43,754 - GFF1451 0.80 -1.1 43,754 - GFF1451 0.80 +0.6 43,754 - GFF1451 0.80 -0.2 43,789 + GFF1451 0.87 +0.6 43,789 + GFF1451 0.87 +1.8 43,822 + -0.1 43,823 - -0.6 43,823 - -1.4 43,823 - -0.7 43,823 - -0.3 43,823 - -0.6 43,980 - -0.2 44,042 + -0.4 44,042 + +0.1 44,043 - -0.2 44,106 - GFF1452 0.11 -0.7 44,237 + GFF1452 0.18 -0.9 44,237 + GFF1452 0.18 +0.3 44,238 - GFF1452 0.18 -1.5 44,238 - GFF1452 0.18 +0.6 44,564 + GFF1452 0.35 +0.5 44,565 - GFF1452 0.35 -0.6 44,565 - GFF1452 0.35 -1.8 44,870 + GFF1452 0.51 -0.4 44,871 - GFF1452 0.51 -0.4 44,891 + GFF1452 0.52 -0.9 44,891 + GFF1452 0.52 +0.3 44,891 + GFF1452 0.52 -0.9 45,598 + GFF1452 0.90 -0.3 45,598 + GFF1452 0.90 -1.4 45,599 - GFF1452 0.90 -1.2 45,599 - GFF1452 0.90 -1.9 45,599 - GFF1452 0.90 +0.1 45,620 + -0.7 45,872 + -0.3 46,205 + GFF1453 0.40 -0.3 46,205 + GFF1453 0.40 -0.2 46,205 + GFF1453 0.40 -0.1 46,205 + GFF1453 0.40 +0.6 46,206 - GFF1453 0.40 +0.2 46,206 - GFF1453 0.40 +0.7 46,206 - GFF1453 0.40 +1.1 46,382 + GFF1453 0.57 +2.5 46,382 + GFF1453 0.57 +0.4 46,383 - GFF1453 0.57 -0.7 46,383 - GFF1453 0.57 +0.4 46,457 + GFF1453 0.65 +0.6 46,457 + GFF1453 0.65 -0.4 46,457 + GFF1453 0.65 -0.5 46,458 - GFF1453 0.65 -1.4 46,458 - GFF1453 0.65 +0.9 46,458 - GFF1453 0.65 -0.9 46,458 - GFF1453 0.65 -0.3 46,458 - GFF1453 0.65 -0.9 46,706 + GFF1453 0.89 +0.1 46,706 + GFF1453 0.89 -0.2 46,706 + GFF1453 0.89 +1.6 46,707 - GFF1453 0.89 -0.1 46,707 - GFF1453 0.89 -1.2 46,707 - GFF1453 0.89 -1.2 46,707 - GFF1453 0.89 -0.4 46,712 + GFF1453 0.90 -0.7 46,713 - GFF1453 0.90 -0.3 46,713 - GFF1453 0.90 +0.1 46,713 - GFF1453 0.90 +0.4
Or see this region's nucleotide sequence