Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1345 and GFF1346 overlap by 1 nucleotides GFF1346 and GFF1347 are separated by 15 nucleotides GFF1347 and GFF1348 are separated by 28 nucleotides GFF1348 and GFF1349 are separated by 9 nucleotides
GFF1345 - 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC), at 688,824 to 690,677
GFF1345
GFF1346 - hypothetical protein, at 690,677 to 690,928
GFF1346
GFF1347 - Dabb, at 690,944 to 691,246
GFF1347
GFF1348 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 691,275 to 692,072
GFF1348
GFF1349 - Thioesterase, at 692,082 to 692,546
GFF1349
Position (kb) 690
691
692 Strain fitness (log2 ratio) -1
0
1 at 689.965 kb on - strand, within GFF1345 at 689.965 kb on - strand, within GFF1345 at 689.994 kb on + strand, within GFF1345 at 689.994 kb on + strand, within GFF1345 at 689.995 kb on - strand, within GFF1345 at 689.995 kb on - strand, within GFF1345 at 689.995 kb on - strand, within GFF1345 at 689.995 kb on - strand, within GFF1345 at 690.156 kb on + strand, within GFF1345 at 690.183 kb on + strand, within GFF1345 at 690.184 kb on - strand, within GFF1345 at 690.184 kb on - strand, within GFF1345 at 690.184 kb on - strand, within GFF1345 at 690.456 kb on + strand, within GFF1345 at 690.456 kb on + strand, within GFF1345 at 690.456 kb on + strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.457 kb on - strand, within GFF1345 at 690.579 kb on + strand at 690.580 kb on - strand at 690.591 kb on + strand at 690.591 kb on + strand at 690.782 kb on + strand, within GFF1346 at 690.782 kb on + strand, within GFF1346 at 690.782 kb on + strand, within GFF1346 at 690.783 kb on - strand, within GFF1346 at 690.783 kb on - strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.899 kb on + strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.900 kb on - strand, within GFF1346 at 690.926 kb on + strand at 691.109 kb on + strand, within GFF1347 at 691.109 kb on + strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.110 kb on - strand, within GFF1347 at 691.161 kb on - strand, within GFF1347 at 691.235 kb on + strand at 691.235 kb on + strand at 691.235 kb on + strand at 691.236 kb on - strand at 691.650 kb on + strand, within GFF1348 at 691.684 kb on - strand, within GFF1348 at 691.773 kb on + strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.774 kb on - strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.836 kb on + strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 691.837 kb on - strand, within GFF1348 at 692.199 kb on + strand, within GFF1349 at 692.200 kb on - strand, within GFF1349 at 692.200 kb on - strand, within GFF1349
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 689,965 - GFF1345 0.62 -0.1 689,965 - GFF1345 0.62 +0.6 689,994 + GFF1345 0.63 -0.6 689,994 + GFF1345 0.63 -0.0 689,995 - GFF1345 0.63 +0.3 689,995 - GFF1345 0.63 -0.5 689,995 - GFF1345 0.63 +0.7 689,995 - GFF1345 0.63 -0.7 690,156 + GFF1345 0.72 -0.6 690,183 + GFF1345 0.73 +0.7 690,184 - GFF1345 0.73 -0.8 690,184 - GFF1345 0.73 -0.0 690,184 - GFF1345 0.73 +0.7 690,456 + GFF1345 0.88 +0.9 690,456 + GFF1345 0.88 -0.0 690,456 + GFF1345 0.88 +0.6 690,457 - GFF1345 0.88 -0.8 690,457 - GFF1345 0.88 -1.7 690,457 - GFF1345 0.88 -0.7 690,457 - GFF1345 0.88 -0.0 690,457 - GFF1345 0.88 -0.5 690,579 + -0.4 690,580 - -0.4 690,591 + -1.7 690,591 + -0.3 690,782 + GFF1346 0.42 -0.7 690,782 + GFF1346 0.42 -1.7 690,782 + GFF1346 0.42 -0.2 690,783 - GFF1346 0.42 -1.7 690,783 - GFF1346 0.42 -0.9 690,899 + GFF1346 0.88 -0.3 690,899 + GFF1346 0.88 -0.1 690,899 + GFF1346 0.88 +0.9 690,899 + GFF1346 0.88 -0.8 690,900 - GFF1346 0.88 +0.3 690,900 - GFF1346 0.88 +0.5 690,900 - GFF1346 0.88 -0.2 690,900 - GFF1346 0.88 -0.4 690,900 - GFF1346 0.88 -0.2 690,900 - GFF1346 0.88 -1.4 690,900 - GFF1346 0.88 -0.3 690,900 - GFF1346 0.88 +0.3 690,926 + -0.0 691,109 + GFF1347 0.54 +1.0 691,109 + GFF1347 0.54 -0.4 691,110 - GFF1347 0.55 -0.4 691,110 - GFF1347 0.55 -0.3 691,110 - GFF1347 0.55 -0.7 691,161 - GFF1347 0.72 -0.1 691,235 + -0.1 691,235 + -0.2 691,235 + +0.5 691,236 - +0.7 691,650 + GFF1348 0.47 -1.5 691,684 - GFF1348 0.51 -0.5 691,773 + GFF1348 0.62 -0.5 691,774 - GFF1348 0.63 +0.1 691,774 - GFF1348 0.63 -0.7 691,836 + GFF1348 0.70 -0.3 691,836 + GFF1348 0.70 +0.1 691,836 + GFF1348 0.70 -0.1 691,837 - GFF1348 0.70 -1.6 691,837 - GFF1348 0.70 -0.3 691,837 - GFF1348 0.70 -0.5 691,837 - GFF1348 0.70 -0.1 692,199 + GFF1349 0.25 -0.1 692,200 - GFF1349 0.25 -1.5 692,200 - GFF1349 0.25 -0.9
Or see this region's nucleotide sequence