Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1288 and GFF1289 are separated by 1 nucleotides GFF1289 and GFF1290 are separated by 19 nucleotides GFF1290 and GFF1291 overlap by 4 nucleotides GFF1291 and GFF1292 are separated by 211 nucleotides
GFF1288 - Sulfate transport system permease protein CysT, at 615,310 to 616,197
GFF1288
GFF1289 - Sulfate transport system permease protein CysW, at 616,199 to 617,101
GFF1289
GFF1290 - Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25), at 617,121 to 618,218
GFF1290
GFF1291 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 618,215 to 618,718
GFF1291
GFF1292 - Macrolide-specific efflux protein MacA, at 618,930 to 620,087
GFF1292
Position (kb) 617
618
619 Strain fitness (log2 ratio) -2
-1
0
1
2 at 616.365 kb on - strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.568 kb on + strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.569 kb on - strand, within GFF1289 at 616.821 kb on - strand, within GFF1289 at 616.821 kb on - strand, within GFF1289 at 616.935 kb on - strand, within GFF1289 at 616.935 kb on - strand, within GFF1289 at 616.943 kb on + strand, within GFF1289 at 616.944 kb on - strand, within GFF1289 at 616.944 kb on - strand, within GFF1289 at 616.944 kb on - strand, within GFF1289 at 617.367 kb on + strand, within GFF1290 at 617.368 kb on - strand, within GFF1290 at 617.821 kb on - strand, within GFF1290 at 617.943 kb on + strand, within GFF1290 at 617.943 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.976 kb on + strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 617.977 kb on - strand, within GFF1290 at 618.193 kb on + strand at 618.193 kb on + strand at 618.193 kb on + strand at 618.193 kb on + strand at 618.194 kb on - strand at 618.194 kb on - strand at 618.208 kb on + strand at 618.208 kb on + strand at 618.208 kb on + strand at 618.829 kb on + strand at 618.830 kb on - strand at 618.840 kb on - strand at 618.887 kb on - strand at 618.938 kb on + strand at 618.963 kb on + strand at 618.963 kb on + strand at 618.964 kb on - strand at 618.964 kb on - strand at 618.964 kb on - strand at 618.964 kb on - strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.041 kb on + strand at 619.042 kb on - strand at 619.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 616,365 - GFF1289 0.18 +0.3 616,568 + GFF1289 0.41 -0.3 616,568 + GFF1289 0.41 -0.6 616,568 + GFF1289 0.41 +0.8 616,568 + GFF1289 0.41 -0.9 616,568 + GFF1289 0.41 -0.3 616,568 + GFF1289 0.41 +0.0 616,568 + GFF1289 0.41 +1.1 616,568 + GFF1289 0.41 +0.0 616,569 - GFF1289 0.41 +0.2 616,569 - GFF1289 0.41 -0.1 616,569 - GFF1289 0.41 -1.2 616,569 - GFF1289 0.41 -0.1 616,569 - GFF1289 0.41 +1.3 616,569 - GFF1289 0.41 +0.4 616,569 - GFF1289 0.41 +0.3 616,569 - GFF1289 0.41 +1.0 616,569 - GFF1289 0.41 -0.5 616,569 - GFF1289 0.41 +2.6 616,569 - GFF1289 0.41 -0.5 616,569 - GFF1289 0.41 -0.1 616,569 - GFF1289 0.41 +0.3 616,569 - GFF1289 0.41 +2.3 616,569 - GFF1289 0.41 +1.3 616,569 - GFF1289 0.41 +0.1 616,821 - GFF1289 0.69 +0.8 616,821 - GFF1289 0.69 -0.7 616,935 - GFF1289 0.82 +1.0 616,935 - GFF1289 0.82 -0.2 616,943 + GFF1289 0.82 +0.7 616,944 - GFF1289 0.83 -0.1 616,944 - GFF1289 0.83 +0.6 616,944 - GFF1289 0.83 +0.3 617,367 + GFF1290 0.22 -0.6 617,368 - GFF1290 0.22 +1.0 617,821 - GFF1290 0.64 -0.1 617,943 + GFF1290 0.75 -0.0 617,943 + GFF1290 0.75 -0.7 617,976 + GFF1290 0.78 +0.3 617,976 + GFF1290 0.78 -0.0 617,976 + GFF1290 0.78 -2.2 617,977 - GFF1290 0.78 -0.2 617,977 - GFF1290 0.78 +0.7 617,977 - GFF1290 0.78 -0.4 618,193 + +1.3 618,193 + -0.0 618,193 + -0.1 618,193 + +0.4 618,194 - +0.3 618,194 - +0.1 618,208 + -0.4 618,208 + -0.7 618,208 + +0.1 618,829 + -0.0 618,830 - -0.9 618,840 - -0.2 618,887 - -0.5 618,938 + -0.2 618,963 + +0.7 618,963 + +0.7 618,964 - -1.7 618,964 - +2.3 618,964 - -0.3 618,964 - +0.4 619,041 + +1.0 619,041 + +0.6 619,041 + -1.4 619,041 + +0.2 619,042 - -0.6 619,042 - +0.6
Or see this region's nucleotide sequence