Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1224 and GFF1225 are separated by 149 nucleotides GFF1225 and GFF1226 are separated by 10 nucleotides
GFF1224 - Transcriptional regulator, LysR family, at 543,691 to 544,584
GFF1224
GFF1225 - Glycine/D-amino acid oxidases (deaminating), at 544,734 to 545,906
GFF1225
GFF1226 - Heavy-chain fibroin (Fragment), at 545,917 to 547,248
GFF1226
Position (kb)
544
545
546 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 543.773 kb on + strand at 543.773 kb on + strand at 543.773 kb on + strand at 543.773 kb on + strand at 543.774 kb on - strand at 543.774 kb on - strand at 543.774 kb on - strand at 543.774 kb on - strand at 543.774 kb on - strand at 543.776 kb on + strand at 543.776 kb on + strand at 543.777 kb on - strand at 543.777 kb on - strand at 543.777 kb on - strand at 543.777 kb on - strand at 543.917 kb on + strand, within GFF1224 at 543.947 kb on + strand, within GFF1224 at 543.947 kb on + strand, within GFF1224 at 543.947 kb on + strand, within GFF1224 at 543.947 kb on + strand, within GFF1224 at 543.948 kb on - strand, within GFF1224 at 543.948 kb on - strand, within GFF1224 at 543.968 kb on + strand, within GFF1224 at 543.968 kb on + strand, within GFF1224 at 543.968 kb on + strand, within GFF1224 at 543.968 kb on + strand, within GFF1224 at 543.968 kb on + strand, within GFF1224 at 543.968 kb on + strand, within GFF1224 at 543.969 kb on - strand, within GFF1224 at 543.969 kb on - strand, within GFF1224 at 544.081 kb on + strand, within GFF1224 at 544.145 kb on + strand, within GFF1224 at 544.145 kb on + strand, within GFF1224 at 544.161 kb on - strand, within GFF1224 at 544.253 kb on - strand, within GFF1224 at 544.380 kb on - strand, within GFF1224 at 544.380 kb on - strand, within GFF1224 at 544.655 kb on - strand at 544.686 kb on + strand at 544.785 kb on + strand at 544.785 kb on + strand at 544.785 kb on + strand at 544.932 kb on + strand, within GFF1225 at 545.022 kb on + strand, within GFF1225 at 545.022 kb on + strand, within GFF1225 at 545.022 kb on + strand, within GFF1225 at 545.022 kb on + strand, within GFF1225 at 545.022 kb on + strand, within GFF1225 at 545.023 kb on - strand, within GFF1225 at 545.023 kb on - strand, within GFF1225 at 545.193 kb on + strand, within GFF1225 at 545.193 kb on + strand, within GFF1225 at 545.193 kb on + strand, within GFF1225 at 545.202 kb on + strand, within GFF1225 at 545.202 kb on + strand, within GFF1225 at 545.202 kb on + strand, within GFF1225 at 545.202 kb on + strand, within GFF1225 at 545.203 kb on - strand, within GFF1225 at 545.203 kb on - strand, within GFF1225 at 545.253 kb on + strand, within GFF1225 at 545.253 kb on + strand, within GFF1225 at 545.253 kb on + strand, within GFF1225 at 545.254 kb on - strand, within GFF1225 at 545.431 kb on + strand, within GFF1225 at 545.431 kb on + strand, within GFF1225 at 545.432 kb on - strand, within GFF1225 at 545.432 kb on - strand, within GFF1225 at 545.432 kb on - strand, within GFF1225 at 545.583 kb on + strand, within GFF1225 at 545.583 kb on + strand, within GFF1225 at 545.583 kb on + strand, within GFF1225 at 545.674 kb on - strand, within GFF1225 at 545.676 kb on + strand, within GFF1225 at 545.676 kb on + strand, within GFF1225 at 545.676 kb on + strand, within GFF1225 at 545.677 kb on - strand, within GFF1225 at 545.677 kb on - strand, within GFF1225 at 545.677 kb on - strand, within GFF1225 at 545.677 kb on - strand, within GFF1225 at 545.677 kb on - strand, within GFF1225 at 545.740 kb on - strand, within GFF1225 at 545.740 kb on - strand, within GFF1225 at 545.904 kb on + strand at 545.904 kb on + strand at 545.905 kb on - strand at 545.905 kb on - strand at 545.999 kb on + strand at 546.000 kb on - strand at 546.000 kb on - strand at 546.000 kb on - strand at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.185 kb on + strand, within GFF1226 at 546.186 kb on - strand, within GFF1226 at 546.186 kb on - strand, within GFF1226 at 546.186 kb on - strand, within GFF1226 at 546.186 kb on - strand, within GFF1226 at 546.186 kb on - strand, within GFF1226 at 546.186 kb on - strand, within GFF1226 at 546.296 kb on + strand, within GFF1226 at 546.296 kb on + strand, within GFF1226 at 546.297 kb on - strand, within GFF1226 at 546.297 kb on - strand, within GFF1226 at 546.297 kb on - strand, within GFF1226 at 546.386 kb on + strand, within GFF1226 at 546.386 kb on + strand, within GFF1226 at 546.545 kb on + strand, within GFF1226 at 546.545 kb on + strand, within GFF1226 at 546.545 kb on + strand, within GFF1226 at 546.545 kb on + strand, within GFF1226 at 546.545 kb on + strand, within GFF1226 at 546.545 kb on + strand, within GFF1226 at 546.545 kb on + strand, within GFF1226 at 546.546 kb on - strand, within GFF1226 at 546.546 kb on - strand, within GFF1226 at 546.546 kb on - strand, within GFF1226 at 546.546 kb on - strand, within GFF1226 at 546.546 kb on - strand, within GFF1226 at 546.546 kb on - strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.548 kb on + strand, within GFF1226 at 546.549 kb on - strand, within GFF1226 at 546.549 kb on - strand, within GFF1226 at 546.549 kb on - strand, within GFF1226 at 546.549 kb on - strand, within GFF1226 at 546.549 kb on - strand, within GFF1226 at 546.549 kb on - strand, within GFF1226 at 546.788 kb on + strand, within GFF1226 at 546.788 kb on + strand, within GFF1226 at 546.788 kb on + strand, within GFF1226 at 546.788 kb on + strand, within GFF1226 at 546.789 kb on - strand, within GFF1226 at 546.789 kb on - strand, within GFF1226 at 546.789 kb on - strand, within GFF1226
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 543,773 + -0.9 543,773 + +2.0 543,773 + +0.6 543,773 + -1.0 543,774 - +0.0 543,774 - -1.0 543,774 - +0.8 543,774 - +0.1 543,774 - +0.1 543,776 + +0.8 543,776 + +0.6 543,777 - -0.1 543,777 - +0.8 543,777 - +0.3 543,777 - +1.7 543,917 + GFF1224 0.25 -0.1 543,947 + GFF1224 0.29 -0.7 543,947 + GFF1224 0.29 -2.2 543,947 + GFF1224 0.29 -0.8 543,947 + GFF1224 0.29 +0.6 543,948 - GFF1224 0.29 +0.4 543,948 - GFF1224 0.29 -0.5 543,968 + GFF1224 0.31 +0.6 543,968 + GFF1224 0.31 +1.3 543,968 + GFF1224 0.31 -1.7 543,968 + GFF1224 0.31 -0.2 543,968 + GFF1224 0.31 -0.8 543,968 + GFF1224 0.31 -0.1 543,969 - GFF1224 0.31 -0.1 543,969 - GFF1224 0.31 +1.3 544,081 + GFF1224 0.44 -0.4 544,145 + GFF1224 0.51 +0.2 544,145 + GFF1224 0.51 -0.5 544,161 - GFF1224 0.53 -1.4 544,253 - GFF1224 0.63 -0.2 544,380 - GFF1224 0.77 -1.7 544,380 - GFF1224 0.77 +0.1 544,655 - +0.4 544,686 + -1.5 544,785 + +0.4 544,785 + -0.0 544,785 + -0.3 544,932 + GFF1225 0.17 -1.7 545,022 + GFF1225 0.25 -0.3 545,022 + GFF1225 0.25 -1.1 545,022 + GFF1225 0.25 -1.4 545,022 + GFF1225 0.25 +0.2 545,022 + GFF1225 0.25 +1.1 545,023 - GFF1225 0.25 -0.0 545,023 - GFF1225 0.25 -0.7 545,193 + GFF1225 0.39 -0.7 545,193 + GFF1225 0.39 -0.3 545,193 + GFF1225 0.39 -0.6 545,202 + GFF1225 0.40 -0.7 545,202 + GFF1225 0.40 +0.3 545,202 + GFF1225 0.40 -0.6 545,202 + GFF1225 0.40 +0.0 545,203 - GFF1225 0.40 -1.4 545,203 - GFF1225 0.40 +1.3 545,253 + GFF1225 0.44 +0.1 545,253 + GFF1225 0.44 +0.2 545,253 + GFF1225 0.44 +0.8 545,254 - GFF1225 0.44 -1.3 545,431 + GFF1225 0.59 -1.0 545,431 + GFF1225 0.59 -0.3 545,432 - GFF1225 0.60 -0.1 545,432 - GFF1225 0.60 -0.5 545,432 - GFF1225 0.60 +0.2 545,583 + GFF1225 0.72 -0.3 545,583 + GFF1225 0.72 -2.0 545,583 + GFF1225 0.72 +0.1 545,674 - GFF1225 0.80 -1.2 545,676 + GFF1225 0.80 -0.6 545,676 + GFF1225 0.80 -0.6 545,676 + GFF1225 0.80 -0.5 545,677 - GFF1225 0.80 -2.2 545,677 - GFF1225 0.80 +0.1 545,677 - GFF1225 0.80 -1.1 545,677 - GFF1225 0.80 +0.3 545,677 - GFF1225 0.80 +0.7 545,740 - GFF1225 0.86 -1.3 545,740 - GFF1225 0.86 -0.4 545,904 + -1.0 545,904 + -0.5 545,905 - +0.3 545,905 - -0.3 545,999 + +0.3 546,000 - +0.2 546,000 - -1.8 546,000 - +0.4 546,185 + GFF1226 0.20 -0.5 546,185 + GFF1226 0.20 -0.1 546,185 + GFF1226 0.20 -0.1 546,185 + GFF1226 0.20 -0.3 546,185 + GFF1226 0.20 +0.1 546,185 + GFF1226 0.20 +0.4 546,185 + GFF1226 0.20 +0.2 546,185 + GFF1226 0.20 +1.7 546,185 + GFF1226 0.20 -0.6 546,186 - GFF1226 0.20 -1.3 546,186 - GFF1226 0.20 -0.0 546,186 - GFF1226 0.20 +0.1 546,186 - GFF1226 0.20 -1.1 546,186 - GFF1226 0.20 +0.5 546,186 - GFF1226 0.20 +0.7 546,296 + GFF1226 0.28 -0.5 546,296 + GFF1226 0.28 +0.7 546,297 - GFF1226 0.29 -0.2 546,297 - GFF1226 0.29 -1.4 546,297 - GFF1226 0.29 -0.6 546,386 + GFF1226 0.35 +0.3 546,386 + GFF1226 0.35 -1.0 546,545 + GFF1226 0.47 +0.4 546,545 + GFF1226 0.47 -1.1 546,545 + GFF1226 0.47 +0.1 546,545 + GFF1226 0.47 -1.9 546,545 + GFF1226 0.47 -0.6 546,545 + GFF1226 0.47 -0.8 546,545 + GFF1226 0.47 +0.6 546,546 - GFF1226 0.47 -0.1 546,546 - GFF1226 0.47 -1.5 546,546 - GFF1226 0.47 +0.0 546,546 - GFF1226 0.47 +0.7 546,546 - GFF1226 0.47 +1.3 546,546 - GFF1226 0.47 -1.0 546,548 + GFF1226 0.47 +0.3 546,548 + GFF1226 0.47 +0.5 546,548 + GFF1226 0.47 -0.9 546,548 + GFF1226 0.47 -1.4 546,548 + GFF1226 0.47 -1.3 546,548 + GFF1226 0.47 +0.1 546,548 + GFF1226 0.47 -0.2 546,548 + GFF1226 0.47 +0.5 546,548 + GFF1226 0.47 -0.1 546,549 - GFF1226 0.47 +0.1 546,549 - GFF1226 0.47 -0.2 546,549 - GFF1226 0.47 +0.4 546,549 - GFF1226 0.47 -0.0 546,549 - GFF1226 0.47 -1.3 546,549 - GFF1226 0.47 -0.3 546,788 + GFF1226 0.65 +0.0 546,788 + GFF1226 0.65 -1.2 546,788 + GFF1226 0.65 -0.5 546,788 + GFF1226 0.65 -0.4 546,789 - GFF1226 0.65 +0.1 546,789 - GFF1226 0.65 -0.8 546,789 - GFF1226 0.65 +0.9
Or see this region's nucleotide sequence