Strain Fitness in Variovorax sp. SCN45 around GFF1195

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1193 and GFF1194 overlap by 27 nucleotidesGFF1194 and GFF1195 overlap by 4 nucleotidesGFF1195 and GFF1196 overlap by 4 nucleotides GFF1193 - Adenylate cyclase (EC 4.6.1.1), at 510,497 to 512,395 GFF1193 GFF1194 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 512,369 to 513,343 GFF1194 GFF1195 - iron-sulphur protein, at 513,340 to 514,575 GFF1195 GFF1196 - BUG/TctC family periplasmic protein, at 514,572 to 515,579 GFF1196 Position (kb) 513 514 515Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 512.360 kb on + strandat 512.360 kb on + strandat 512.360 kb on + strandat 512.360 kb on + strandat 512.361 kb on - strandat 512.370 kb on + strandat 512.370 kb on + strandat 512.370 kb on + strandat 512.370 kb on + strandat 512.370 kb on + strandat 512.371 kb on - strandat 512.371 kb on - strandat 512.371 kb on - strandat 512.457 kb on + strandat 512.457 kb on + strandat 512.457 kb on + strandat 512.457 kb on + strandat 512.458 kb on - strandat 512.458 kb on - strandat 512.458 kb on - strandat 512.458 kb on - strandat 512.671 kb on - strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.757 kb on + strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.758 kb on - strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.843 kb on + strand, within GFF1194at 512.844 kb on - strand, within GFF1194at 512.844 kb on - strand, within GFF1194at 512.844 kb on - strand, within GFF1194at 512.844 kb on - strand, within GFF1194at 512.844 kb on - strand, within GFF1194at 512.844 kb on - strand, within GFF1194at 512.844 kb on - strand, within GFF1194at 513.174 kb on + strand, within GFF1194at 513.175 kb on - strand, within GFF1194at 513.545 kb on + strand, within GFF1195at 513.545 kb on + strand, within GFF1195at 513.546 kb on - strand, within GFF1195at 513.659 kb on + strand, within GFF1195at 513.659 kb on + strand, within GFF1195at 513.659 kb on + strand, within GFF1195at 513.660 kb on - strand, within GFF1195at 513.660 kb on - strand, within GFF1195at 513.660 kb on - strand, within GFF1195at 513.660 kb on - strand, within GFF1195at 513.660 kb on - strand, within GFF1195at 513.947 kb on + strand, within GFF1195at 513.947 kb on + strand, within GFF1195at 513.947 kb on + strand, within GFF1195at 513.947 kb on + strand, within GFF1195at 513.947 kb on + strand, within GFF1195at 514.052 kb on + strand, within GFF1195at 514.052 kb on + strand, within GFF1195at 514.053 kb on - strand, within GFF1195at 514.091 kb on + strand, within GFF1195at 514.091 kb on + strand, within GFF1195at 514.092 kb on - strand, within GFF1195at 514.092 kb on - strand, within GFF1195at 514.092 kb on - strand, within GFF1195at 514.227 kb on - strand, within GFF1195at 514.227 kb on - strand, within GFF1195at 514.227 kb on - strand, within GFF1195at 514.262 kb on + strand, within GFF1195at 514.262 kb on + strand, within GFF1195at 514.262 kb on + strand, within GFF1195at 514.262 kb on + strand, within GFF1195at 514.262 kb on + strand, within GFF1195at 514.262 kb on + strand, within GFF1195at 514.263 kb on - strand, within GFF1195at 514.263 kb on - strand, within GFF1195at 514.265 kb on + strand, within GFF1195at 514.266 kb on - strand, within GFF1195at 514.412 kb on + strand, within GFF1195at 514.412 kb on + strand, within GFF1195at 514.412 kb on + strand, within GFF1195at 514.413 kb on - strand, within GFF1195at 514.633 kb on + strandat 514.633 kb on + strandat 514.633 kb on + strandat 514.633 kb on + strandat 514.633 kb on + strandat 514.633 kb on + strandat 514.633 kb on + strandat 514.634 kb on - strandat 514.634 kb on - strandat 514.634 kb on - strandat 514.634 kb on - strandat 514.780 kb on + strand, within GFF1196at 514.780 kb on + strand, within GFF1196at 514.780 kb on + strand, within GFF1196at 514.781 kb on - strand, within GFF1196at 514.993 kb on + strand, within GFF1196at 514.993 kb on + strand, within GFF1196at 514.993 kb on + strand, within GFF1196at 514.993 kb on + strand, within GFF1196at 514.994 kb on - strand, within GFF1196at 515.081 kb on - strand, within GFF1196at 515.081 kb on - strand, within GFF1196at 515.081 kb on - strand, within GFF1196at 515.081 kb on - strand, within GFF1196at 515.108 kb on - strand, within GFF1196at 515.108 kb on - strand, within GFF1196at 515.215 kb on + strand, within GFF1196at 515.216 kb on - strand, within GFF1196at 515.216 kb on - strand, within GFF1196at 515.216 kb on - strand, within GFF1196at 515.282 kb on - strand, within GFF1196at 515.393 kb on - strand, within GFF1196

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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512,360 + -0.0
512,360 + -0.1
512,360 + -0.0
512,360 + +0.7
512,361 - -0.5
512,370 + -0.2
512,370 + +0.0
512,370 + +0.1
512,370 + +0.7
512,370 + +2.9
512,371 - -0.4
512,371 - +0.7
512,371 - +0.8
512,457 + -0.6
512,457 + +0.9
512,457 + -0.2
512,457 + -0.4
512,458 - -0.3
512,458 - -1.8
512,458 - +0.1
512,458 - -0.4
512,671 - GFF1194 0.31 +0.2
512,757 + GFF1194 0.40 -0.5
512,757 + GFF1194 0.40 -0.2
512,757 + GFF1194 0.40 -0.4
512,757 + GFF1194 0.40 -0.4
512,757 + GFF1194 0.40 +0.8
512,757 + GFF1194 0.40 -0.5
512,757 + GFF1194 0.40 -0.4
512,757 + GFF1194 0.40 -0.1
512,757 + GFF1194 0.40 -0.1
512,757 + GFF1194 0.40 +0.5
512,757 + GFF1194 0.40 -1.0
512,758 - GFF1194 0.40 +0.3
512,758 - GFF1194 0.40 -1.2
512,758 - GFF1194 0.40 -0.3
512,758 - GFF1194 0.40 +0.4
512,758 - GFF1194 0.40 -1.3
512,758 - GFF1194 0.40 +1.9
512,758 - GFF1194 0.40 +1.3
512,758 - GFF1194 0.40 +0.4
512,758 - GFF1194 0.40 -1.3
512,758 - GFF1194 0.40 -0.2
512,758 - GFF1194 0.40 -0.3
512,758 - GFF1194 0.40 +0.1
512,758 - GFF1194 0.40 -0.5
512,843 + GFF1194 0.49 -1.0
512,843 + GFF1194 0.49 -0.8
512,843 + GFF1194 0.49 +1.0
512,843 + GFF1194 0.49 -0.3
512,843 + GFF1194 0.49 +0.2
512,843 + GFF1194 0.49 +0.1
512,843 + GFF1194 0.49 -0.3
512,843 + GFF1194 0.49 -0.4
512,844 - GFF1194 0.49 -0.1
512,844 - GFF1194 0.49 -0.3
512,844 - GFF1194 0.49 -1.7
512,844 - GFF1194 0.49 -0.2
512,844 - GFF1194 0.49 -0.3
512,844 - GFF1194 0.49 -1.0
512,844 - GFF1194 0.49 +0.4
513,174 + GFF1194 0.83 +1.3
513,175 - GFF1194 0.83 +2.9
513,545 + GFF1195 0.17 +0.7
513,545 + GFF1195 0.17 -0.3
513,546 - GFF1195 0.17 +0.4
513,659 + GFF1195 0.26 +0.4
513,659 + GFF1195 0.26 -0.6
513,659 + GFF1195 0.26 -2.2
513,660 - GFF1195 0.26 +0.5
513,660 - GFF1195 0.26 -0.5
513,660 - GFF1195 0.26 +0.5
513,660 - GFF1195 0.26 -0.9
513,660 - GFF1195 0.26 -0.0
513,947 + GFF1195 0.49 -0.5
513,947 + GFF1195 0.49 +0.0
513,947 + GFF1195 0.49 -0.8
513,947 + GFF1195 0.49 -0.6
513,947 + GFF1195 0.49 -1.6
514,052 + GFF1195 0.58 -3.0
514,052 + GFF1195 0.58 +0.2
514,053 - GFF1195 0.58 +0.5
514,091 + GFF1195 0.61 -0.9
514,091 + GFF1195 0.61 -1.0
514,092 - GFF1195 0.61 +0.2
514,092 - GFF1195 0.61 -0.7
514,092 - GFF1195 0.61 -0.4
514,227 - GFF1195 0.72 +0.5
514,227 - GFF1195 0.72 -0.5
514,227 - GFF1195 0.72 -0.8
514,262 + GFF1195 0.75 -1.7
514,262 + GFF1195 0.75 +0.4
514,262 + GFF1195 0.75 +0.1
514,262 + GFF1195 0.75 -0.1
514,262 + GFF1195 0.75 -0.2
514,262 + GFF1195 0.75 -0.6
514,263 - GFF1195 0.75 +0.0
514,263 - GFF1195 0.75 -0.4
514,265 + GFF1195 0.75 +0.0
514,266 - GFF1195 0.75 +0.3
514,412 + GFF1195 0.87 +0.6
514,412 + GFF1195 0.87 -0.3
514,412 + GFF1195 0.87 -0.3
514,413 - GFF1195 0.87 +1.9
514,633 + -0.0
514,633 + -0.0
514,633 + +0.0
514,633 + +0.4
514,633 + -1.2
514,633 + +0.3
514,633 + +0.0
514,634 - +0.2
514,634 - +2.3
514,634 - -0.1
514,634 - -0.7
514,780 + GFF1196 0.21 -0.2
514,780 + GFF1196 0.21 +0.3
514,780 + GFF1196 0.21 +0.3
514,781 - GFF1196 0.21 -0.8
514,993 + GFF1196 0.42 -0.2
514,993 + GFF1196 0.42 +0.5
514,993 + GFF1196 0.42 +0.0
514,993 + GFF1196 0.42 -0.5
514,994 - GFF1196 0.42 -0.0
515,081 - GFF1196 0.50 -0.1
515,081 - GFF1196 0.50 -0.8
515,081 - GFF1196 0.50 -0.8
515,081 - GFF1196 0.50 -1.0
515,108 - GFF1196 0.53 +0.2
515,108 - GFF1196 0.53 +0.2
515,215 + GFF1196 0.64 -2.5
515,216 - GFF1196 0.64 -0.3
515,216 - GFF1196 0.64 -1.0
515,216 - GFF1196 0.64 +0.5
515,282 - GFF1196 0.70 -0.3
515,393 - GFF1196 0.81 -1.5

Or see this region's nucleotide sequence