Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF109 and GFF110 are separated by 10 nucleotides GFF110 and GFF111 overlap by 4 nucleotides
GFF109 - Protein lysine acetyltransferase Pat (EC 2.3.1.-), at 136,527 to 139,229
GFF109
GFF110 - pyridoxal-phosphate dependent enzyme family protein, at 139,240 to 140,199
GFF110
GFF111 - hypothetical protein, at 140,196 to 141,221
GFF111
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-1
0
1
2
3 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.280 kb on + strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.281 kb on - strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.282 kb on + strand, within GFF109 at 138.283 kb on - strand, within GFF109 at 138.283 kb on - strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.732 kb on + strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.733 kb on - strand, within GFF109 at 138.879 kb on + strand, within GFF109 at 138.978 kb on + strand at 138.978 kb on + strand at 138.979 kb on - strand at 138.979 kb on - strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.218 kb on + strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.219 kb on - strand at 139.227 kb on + strand at 139.442 kb on + strand, within GFF110 at 139.442 kb on + strand, within GFF110 at 139.443 kb on - strand, within GFF110 at 139.443 kb on - strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.553 kb on + strand, within GFF110 at 139.554 kb on - strand, within GFF110 at 139.745 kb on + strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.746 kb on - strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.928 kb on + strand, within GFF110 at 139.929 kb on - strand, within GFF110 at 140.121 kb on + strand at 140.132 kb on + strand at 140.132 kb on + strand at 140.278 kb on + strand at 140.278 kb on + strand at 140.278 kb on + strand at 140.279 kb on - strand at 140.279 kb on - strand at 140.404 kb on + strand, within GFF111 at 140.404 kb on + strand, within GFF111 at 140.794 kb on + strand, within GFF111 at 140.794 kb on + strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 141.028 kb on + strand, within GFF111
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 138,280 + GFF109 0.65 +0.4 138,280 + GFF109 0.65 -0.5 138,280 + GFF109 0.65 +0.9 138,280 + GFF109 0.65 +0.5 138,280 + GFF109 0.65 +0.4 138,281 - GFF109 0.65 +0.3 138,281 - GFF109 0.65 -0.6 138,281 - GFF109 0.65 -0.0 138,282 + GFF109 0.65 -0.6 138,282 + GFF109 0.65 -1.1 138,282 + GFF109 0.65 -0.2 138,282 + GFF109 0.65 -0.6 138,282 + GFF109 0.65 -0.2 138,283 - GFF109 0.65 -1.1 138,283 - GFF109 0.65 -0.1 138,732 + GFF109 0.82 -0.1 138,732 + GFF109 0.82 +1.2 138,732 + GFF109 0.82 -0.2 138,732 + GFF109 0.82 -0.1 138,732 + GFF109 0.82 +1.4 138,733 - GFF109 0.82 +0.5 138,733 - GFF109 0.82 -1.1 138,733 - GFF109 0.82 -1.3 138,879 + GFF109 0.87 -0.8 138,978 + +0.0 138,978 + -0.8 138,979 - -1.2 138,979 - -0.6 139,218 + +0.0 139,218 + +0.7 139,218 + +0.4 139,218 + -0.2 139,218 + -1.0 139,219 - -0.1 139,219 - -0.0 139,219 - +0.3 139,219 - -1.4 139,219 - -0.2 139,227 + +1.2 139,442 + GFF110 0.21 +0.6 139,442 + GFF110 0.21 -0.5 139,443 - GFF110 0.21 -0.8 139,443 - GFF110 0.21 -0.7 139,553 + GFF110 0.33 -0.0 139,553 + GFF110 0.33 +0.1 139,553 + GFF110 0.33 -0.2 139,553 + GFF110 0.33 +1.2 139,554 - GFF110 0.33 -0.1 139,745 + GFF110 0.53 -0.3 139,746 - GFF110 0.53 -0.0 139,746 - GFF110 0.53 +0.3 139,746 - GFF110 0.53 -0.4 139,746 - GFF110 0.53 -0.8 139,928 + GFF110 0.72 -0.2 139,928 + GFF110 0.72 +0.7 139,928 + GFF110 0.72 +3.2 139,929 - GFF110 0.72 -0.3 140,121 + +0.8 140,132 + -1.6 140,132 + +0.5 140,278 + -0.1 140,278 + +0.3 140,278 + -0.1 140,279 - -0.1 140,279 - +0.2 140,404 + GFF111 0.20 -1.1 140,404 + GFF111 0.20 -0.3 140,794 + GFF111 0.58 -1.6 140,794 + GFF111 0.58 +0.0 140,795 - GFF111 0.58 -0.1 140,795 - GFF111 0.58 +0.5 140,795 - GFF111 0.58 +1.1 140,795 - GFF111 0.58 +1.4 141,028 + GFF111 0.81 -0.8
Or see this region's nucleotide sequence