Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1059 and GFF1060 overlap by 4 nucleotides GFF1060 and GFF1061 overlap by 22 nucleotides GFF1061 and GFF1062 overlap by 1 nucleotides GFF1062 and GFF1063 are separated by 41 nucleotides
GFF1059 - Hydroxymethylpyrimidine ABC transporter, ATPase component, at 373,343 to 374,194
GFF1059
GFF1060 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 374,191 to 374,949
GFF1060
GFF1061 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 374,928 to 375,686
GFF1061
GFF1062 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 375,686 to 376,462
GFF1062
GFF1063 - dioxygenase, TauD/TfdA family, at 376,504 to 377,343
GFF1063
Position (kb)
374
375
376 Strain fitness (log2 ratio)
-2
-1
0
1 at 374.266 kb on + strand at 374.266 kb on + strand at 374.266 kb on + strand at 374.266 kb on + strand at 374.266 kb on + strand at 374.266 kb on + strand at 374.267 kb on - strand, within GFF1060 at 374.296 kb on + strand, within GFF1060 at 374.443 kb on + strand, within GFF1060 at 374.443 kb on + strand, within GFF1060 at 374.444 kb on - strand, within GFF1060 at 375.141 kb on + strand, within GFF1061 at 375.248 kb on + strand, within GFF1061 at 375.249 kb on - strand, within GFF1061 at 375.259 kb on - strand, within GFF1061 at 375.561 kb on + strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.562 kb on - strand, within GFF1061 at 375.761 kb on + strand at 375.800 kb on + strand, within GFF1062 at 375.800 kb on + strand, within GFF1062 at 375.801 kb on - strand, within GFF1062 at 375.801 kb on - strand, within GFF1062 at 375.971 kb on - strand, within GFF1062 at 376.118 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.253 kb on + strand, within GFF1062 at 376.254 kb on - strand, within GFF1062 at 376.256 kb on + strand, within GFF1062 at 376.256 kb on + strand, within GFF1062 at 376.257 kb on - strand, within GFF1062 at 376.257 kb on - strand, within GFF1062 at 376.326 kb on - strand, within GFF1062
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 374,266 + +0.6 374,266 + -0.5 374,266 + -1.0 374,266 + +1.0 374,266 + +0.1 374,266 + -0.5 374,267 - GFF1060 0.10 -0.7 374,296 + GFF1060 0.14 -0.3 374,443 + GFF1060 0.33 +1.7 374,443 + GFF1060 0.33 -0.5 374,444 - GFF1060 0.33 -0.4 375,141 + GFF1061 0.28 +0.7 375,248 + GFF1061 0.42 -0.2 375,249 - GFF1061 0.42 -0.7 375,259 - GFF1061 0.44 -0.1 375,561 + GFF1061 0.83 +0.7 375,562 - GFF1061 0.84 +0.5 375,562 - GFF1061 0.84 -0.1 375,562 - GFF1061 0.84 -1.8 375,562 - GFF1061 0.84 +0.4 375,562 - GFF1061 0.84 +0.2 375,761 + -0.3 375,800 + GFF1062 0.15 -0.7 375,800 + GFF1062 0.15 -0.5 375,801 - GFF1062 0.15 -0.1 375,801 - GFF1062 0.15 +0.2 375,971 - GFF1062 0.37 -0.5 376,118 + GFF1062 0.56 -0.3 376,253 + GFF1062 0.73 -0.0 376,253 + GFF1062 0.73 -0.6 376,253 + GFF1062 0.73 +0.2 376,253 + GFF1062 0.73 +0.9 376,254 - GFF1062 0.73 +0.5 376,256 + GFF1062 0.73 -0.5 376,256 + GFF1062 0.73 -0.2 376,257 - GFF1062 0.73 -0.3 376,257 - GFF1062 0.73 -0.1 376,326 - GFF1062 0.82 -1.0
Or see this region's nucleotide sequence