Strain Fitness in Escherichia coli ECOR38 around HEPCGN_24930

Experiment: K20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntprmC and sirB2 overlap by 4 nucleotidessirB2 and sirB1 are separated by 3 nucleotidessirB1 and kdsA are separated by 35 nucleotides HEPCGN_24920: prmC - peptide chain release factor N(5)-glutamine methyltransferase, at 3,740,265 to 3,741,098 prmC HEPCGN_24925: sirB2 - invasion regulator SirB2, at 3,741,095 to 3,741,487 sirB2 HEPCGN_24930: sirB1 - invasion regulator SirB1, at 3,741,491 to 3,742,300 sirB1 HEPCGN_24935: kdsA - 3-deoxy-8-phosphooctulonate synthase, at 3,742,336 to 3,743,190 kdsA Position (kb) 3741 3742 3743Strain fitness (log2 ratio) -1 0 1 2 3at 3740.509 kb on + strand, within prmCat 3740.688 kb on - strand, within prmCat 3740.717 kb on + strand, within prmCat 3740.717 kb on + strand, within prmCat 3740.821 kb on - strand, within prmCat 3740.822 kb on + strand, within prmCat 3741.151 kb on - strand, within sirB2at 3741.160 kb on - strand, within sirB2at 3741.177 kb on + strand, within sirB2at 3741.278 kb on - strand, within sirB2at 3741.365 kb on - strand, within sirB2at 3741.385 kb on + strand, within sirB2at 3741.385 kb on + strand, within sirB2at 3741.385 kb on + strand, within sirB2at 3741.441 kb on - strand, within sirB2at 3741.477 kb on + strandat 3741.486 kb on - strandat 3741.486 kb on - strandat 3741.518 kb on - strandat 3741.616 kb on + strand, within sirB1at 3741.689 kb on + strand, within sirB1at 3741.744 kb on + strand, within sirB1at 3741.744 kb on + strand, within sirB1at 3741.762 kb on + strand, within sirB1at 3741.792 kb on + strand, within sirB1at 3741.792 kb on + strand, within sirB1at 3741.805 kb on - strand, within sirB1at 3741.992 kb on + strand, within sirB1at 3741.992 kb on + strand, within sirB1at 3742.203 kb on + strand, within sirB1at 3742.279 kb on + strandat 3742.302 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K20
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3,740,509 + prmC HEPCGN_24920 0.29 -0.7
3,740,688 - prmC HEPCGN_24920 0.51 +0.2
3,740,717 + prmC HEPCGN_24920 0.54 +3.2
3,740,717 + prmC HEPCGN_24920 0.54 +0.6
3,740,821 - prmC HEPCGN_24920 0.67 -0.4
3,740,822 + prmC HEPCGN_24920 0.67 +2.6
3,741,151 - sirB2 HEPCGN_24925 0.14 -0.2
3,741,160 - sirB2 HEPCGN_24925 0.17 +0.7
3,741,177 + sirB2 HEPCGN_24925 0.21 +0.1
3,741,278 - sirB2 HEPCGN_24925 0.47 -1.6
3,741,365 - sirB2 HEPCGN_24925 0.69 +0.0
3,741,385 + sirB2 HEPCGN_24925 0.74 -0.7
3,741,385 + sirB2 HEPCGN_24925 0.74 -0.8
3,741,385 + sirB2 HEPCGN_24925 0.74 +0.6
3,741,441 - sirB2 HEPCGN_24925 0.88 -0.3
3,741,477 + -0.4
3,741,486 - -0.4
3,741,486 - -0.3
3,741,518 - -0.6
3,741,616 + sirB1 HEPCGN_24930 0.15 +0.8
3,741,689 + sirB1 HEPCGN_24930 0.24 +0.8
3,741,744 + sirB1 HEPCGN_24930 0.31 +0.2
3,741,744 + sirB1 HEPCGN_24930 0.31 +0.2
3,741,762 + sirB1 HEPCGN_24930 0.33 +1.3
3,741,792 + sirB1 HEPCGN_24930 0.37 +0.6
3,741,792 + sirB1 HEPCGN_24930 0.37 -0.4
3,741,805 - sirB1 HEPCGN_24930 0.39 +2.2
3,741,992 + sirB1 HEPCGN_24930 0.62 -0.7
3,741,992 + sirB1 HEPCGN_24930 0.62 -0.1
3,742,203 + sirB1 HEPCGN_24930 0.88 +0.3
3,742,279 + -0.3
3,742,302 + +0.7

Or see this region's nucleotide sequence