Experiment: K20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt macB and cspD are separated by 72 nucleotides cspD and yljB are separated by 149 nucleotides yljB and clpS are separated by 98 nucleotides clpS and clpA are separated by 30 nucleotides clpA and Ser_trna are separated by 585 nucleotides Ser_trna and infA are separated by 253 nucleotides
HEPCGN_22880: macB - macrolide ABC transporter ATP-binding protein/permease MacB, at 3,353,602 to 3,355,548
macB
HEPCGN_22885: cspD - cold shock-like protein CspD, at 3,355,621 to 3,355,845
cspD
HEPCGN_22890: yljB - Protein YljB, at 3,355,995 to 3,356,069
yljB
HEPCGN_22895: clpS - ATP-dependent Clp protease adapter ClpS, at 3,356,168 to 3,356,488
clpS
HEPCGN_22900: clpA - ATP-dependent Clp protease ATP-binding subunit ClpA, at 3,356,519 to 3,358,795
clpA
HEPCGN_22905: Ser_trna - tRNA-Ser(gga), at 3,359,381 to 3,359,468
_trna
HEPCGN_22910: infA - translation initiation factor IF-1, at 3,359,722 to 3,359,940
infA
Position (kb)
3356
3357
3358
3359 Strain fitness (log2 ratio)
-2
-1
0
1 at 3355.527 kb on - strand at 3355.594 kb on + strand at 3355.595 kb on - strand at 3355.601 kb on + strand at 3355.602 kb on - strand at 3355.623 kb on - strand at 3355.639 kb on + strand at 3355.639 kb on + strand at 3355.720 kb on + strand, within cspD at 3355.827 kb on + strand at 3355.827 kb on + strand at 3355.828 kb on - strand at 3355.828 kb on - strand at 3355.828 kb on - strand at 3355.871 kb on + strand at 3355.877 kb on + strand at 3355.878 kb on - strand at 3355.878 kb on - strand at 3355.887 kb on + strand at 3355.994 kb on + strand at 3355.995 kb on - strand at 3355.995 kb on - strand at 3356.064 kb on - strand at 3356.070 kb on + strand at 3356.075 kb on - strand at 3356.075 kb on - strand at 3356.107 kb on + strand at 3356.107 kb on + strand at 3356.159 kb on + strand at 3356.159 kb on + strand at 3356.173 kb on + strand at 3356.233 kb on - strand, within clpS at 3356.245 kb on + strand, within clpS at 3356.246 kb on - strand, within clpS at 3356.246 kb on - strand, within clpS at 3356.246 kb on - strand, within clpS at 3356.276 kb on + strand, within clpS at 3356.324 kb on + strand, within clpS at 3356.325 kb on - strand, within clpS at 3356.331 kb on + strand, within clpS at 3356.438 kb on + strand, within clpS at 3356.470 kb on + strand at 3356.498 kb on + strand at 3356.519 kb on - strand at 3356.551 kb on + strand at 3356.590 kb on + strand at 3356.590 kb on + strand at 3356.591 kb on - strand at 3356.702 kb on - strand at 3357.113 kb on - strand, within clpA at 3357.319 kb on + strand, within clpA at 3357.320 kb on - strand, within clpA at 3357.367 kb on + strand, within clpA at 3357.368 kb on - strand, within clpA at 3357.557 kb on - strand, within clpA at 3357.612 kb on - strand, within clpA at 3357.721 kb on + strand, within clpA at 3357.973 kb on + strand, within clpA at 3358.079 kb on - strand, within clpA at 3358.089 kb on - strand, within clpA at 3358.271 kb on - strand, within clpA at 3358.387 kb on + strand, within clpA at 3358.459 kb on + strand, within clpA at 3358.748 kb on + strand at 3358.899 kb on + strand at 3358.914 kb on + strand at 3358.914 kb on + strand at 3358.914 kb on + strand at 3358.915 kb on - strand at 3358.979 kb on - strand at 3359.000 kb on + strand at 3359.004 kb on - strand at 3359.033 kb on - strand at 3359.093 kb on - strand at 3359.111 kb on + strand at 3359.556 kb on + strand at 3359.561 kb on - strand at 3359.594 kb on + strand at 3359.594 kb on + strand at 3359.594 kb on + strand at 3359.596 kb on + strand at 3359.597 kb on - strand at 3359.623 kb on + strand at 3359.623 kb on + strand at 3359.623 kb on + strand at 3359.624 kb on - strand at 3359.625 kb on + strand at 3359.625 kb on + strand at 3359.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K20 remove 3,355,527 - -0.4 3,355,594 + -0.2 3,355,595 - -0.1 3,355,601 + +0.1 3,355,602 - +0.1 3,355,623 - +0.6 3,355,639 + +0.3 3,355,639 + -0.5 3,355,720 + cspD HEPCGN_22885 0.44 -0.8 3,355,827 + -1.8 3,355,827 + +0.4 3,355,828 - +0.7 3,355,828 - -0.0 3,355,828 - +0.1 3,355,871 + +1.1 3,355,877 + +1.0 3,355,878 - +0.1 3,355,878 - +0.8 3,355,887 + +1.0 3,355,994 + -1.6 3,355,995 - -1.2 3,355,995 - +1.1 3,356,064 - -0.5 3,356,070 + +0.1 3,356,075 - +0.7 3,356,075 - -0.6 3,356,107 + +0.1 3,356,107 + +0.8 3,356,159 + -0.4 3,356,159 + -0.2 3,356,173 + -0.3 3,356,233 - clpS HEPCGN_22895 0.20 -0.3 3,356,245 + clpS HEPCGN_22895 0.24 +0.1 3,356,246 - clpS HEPCGN_22895 0.24 +0.0 3,356,246 - clpS HEPCGN_22895 0.24 -0.8 3,356,246 - clpS HEPCGN_22895 0.24 +0.6 3,356,276 + clpS HEPCGN_22895 0.34 -0.3 3,356,324 + clpS HEPCGN_22895 0.49 -1.1 3,356,325 - clpS HEPCGN_22895 0.49 +1.7 3,356,331 + clpS HEPCGN_22895 0.51 -1.3 3,356,438 + clpS HEPCGN_22895 0.84 +0.2 3,356,470 + -0.7 3,356,498 + -0.2 3,356,519 - +0.1 3,356,551 + -0.0 3,356,590 + +0.7 3,356,590 + +0.5 3,356,591 - +0.1 3,356,702 - -0.1 3,357,113 - clpA HEPCGN_22900 0.26 +0.6 3,357,319 + clpA HEPCGN_22900 0.35 +0.9 3,357,320 - clpA HEPCGN_22900 0.35 +1.1 3,357,367 + clpA HEPCGN_22900 0.37 -0.4 3,357,368 - clpA HEPCGN_22900 0.37 +0.1 3,357,557 - clpA HEPCGN_22900 0.46 +0.3 3,357,612 - clpA HEPCGN_22900 0.48 +0.2 3,357,721 + clpA HEPCGN_22900 0.53 +0.4 3,357,973 + clpA HEPCGN_22900 0.64 -0.3 3,358,079 - clpA HEPCGN_22900 0.69 -0.3 3,358,089 - clpA HEPCGN_22900 0.69 -0.4 3,358,271 - clpA HEPCGN_22900 0.77 +0.5 3,358,387 + clpA HEPCGN_22900 0.82 -0.2 3,358,459 + clpA HEPCGN_22900 0.85 +0.7 3,358,748 + -0.4 3,358,899 + -0.3 3,358,914 + -0.7 3,358,914 + +1.2 3,358,914 + +0.3 3,358,915 - -1.7 3,358,979 - +0.4 3,359,000 + +1.7 3,359,004 - -0.9 3,359,033 - -0.8 3,359,093 - +0.4 3,359,111 + +0.6 3,359,556 + +1.0 3,359,561 - +0.1 3,359,594 + +0.6 3,359,594 + -1.1 3,359,594 + -0.5 3,359,596 + +0.3 3,359,597 - +0.1 3,359,623 + -0.7 3,359,623 + -0.4 3,359,623 + -0.1 3,359,624 - +0.5 3,359,625 + -0.4 3,359,625 + +0.1 3,359,647 + +0.5
Or see this region's nucleotide sequence