Experiment: K20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfcH and folX are separated by 20 nucleotides folX and yfcG are separated by 56 nucleotides yfcG and yfcF are separated by 135 nucleotides yfcF and yfcE are separated by 55 nucleotides
HEPCGN_21960: yfcH - Epimerase family protein YfcH, at 3,147,856 to 3,148,749
yfcH
HEPCGN_21965: folX - dihydroneopterin triphosphate 2'-epimerase, at 3,148,770 to 3,149,132
folX
HEPCGN_21970: yfcG - GSH-dependent disulfide bond oxidoreductase, at 3,149,189 to 3,149,836
yfcG
HEPCGN_21975: yfcF - glutathione transferase, at 3,149,972 to 3,150,616
yfcF
HEPCGN_21980: yfcE - phosphodiesterase, at 3,150,672 to 3,151,223
yfcE
Position (kb)
3149
3150 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3148.256 kb on - strand, within yfcH at 3148.387 kb on + strand, within yfcH at 3148.387 kb on + strand, within yfcH at 3148.388 kb on - strand, within yfcH at 3148.397 kb on + strand, within yfcH at 3148.426 kb on + strand, within yfcH at 3148.453 kb on - strand, within yfcH at 3148.530 kb on + strand, within yfcH at 3148.585 kb on - strand, within yfcH at 3148.610 kb on - strand, within yfcH at 3148.718 kb on - strand at 3148.741 kb on + strand at 3148.850 kb on - strand, within folX at 3148.930 kb on - strand, within folX at 3148.930 kb on - strand, within folX at 3148.964 kb on - strand, within folX at 3149.084 kb on + strand, within folX at 3149.103 kb on + strand at 3149.111 kb on + strand at 3149.111 kb on + strand at 3149.157 kb on - strand at 3149.157 kb on - strand at 3149.166 kb on + strand at 3149.169 kb on + strand at 3149.408 kb on + strand, within yfcG at 3149.409 kb on - strand, within yfcG at 3149.482 kb on + strand, within yfcG at 3149.534 kb on + strand, within yfcG at 3149.543 kb on + strand, within yfcG at 3149.544 kb on - strand, within yfcG at 3149.823 kb on + strand at 3149.854 kb on - strand at 3149.875 kb on - strand at 3149.877 kb on - strand at 3149.934 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.970 kb on + strand at 3149.971 kb on - strand at 3149.977 kb on + strand at 3149.978 kb on - strand at 3150.023 kb on + strand at 3150.023 kb on + strand at 3150.023 kb on + strand at 3150.073 kb on + strand, within yfcF at 3150.156 kb on + strand, within yfcF at 3150.241 kb on + strand, within yfcF at 3150.242 kb on - strand, within yfcF at 3150.476 kb on - strand, within yfcF at 3150.510 kb on + strand, within yfcF at 3150.511 kb on - strand, within yfcF at 3150.641 kb on + strand at 3150.641 kb on + strand at 3150.809 kb on - strand, within yfcE
Per-strain Table
Position Strand Gene LocusTag Fraction K20 remove 3,148,256 - yfcH HEPCGN_21960 0.45 -0.4 3,148,387 + yfcH HEPCGN_21960 0.59 -0.5 3,148,387 + yfcH HEPCGN_21960 0.59 -1.0 3,148,388 - yfcH HEPCGN_21960 0.60 -1.2 3,148,397 + yfcH HEPCGN_21960 0.61 +0.3 3,148,426 + yfcH HEPCGN_21960 0.64 +0.1 3,148,453 - yfcH HEPCGN_21960 0.67 -0.5 3,148,530 + yfcH HEPCGN_21960 0.75 -1.9 3,148,585 - yfcH HEPCGN_21960 0.82 +1.8 3,148,610 - yfcH HEPCGN_21960 0.84 +0.0 3,148,718 - -0.5 3,148,741 + -0.1 3,148,850 - folX HEPCGN_21965 0.22 -0.2 3,148,930 - folX HEPCGN_21965 0.44 +0.0 3,148,930 - folX HEPCGN_21965 0.44 +0.4 3,148,964 - folX HEPCGN_21965 0.53 +0.4 3,149,084 + folX HEPCGN_21965 0.87 -1.6 3,149,103 + -0.5 3,149,111 + -0.5 3,149,111 + +0.1 3,149,157 - +0.1 3,149,157 - +1.4 3,149,166 + -1.1 3,149,169 + -0.3 3,149,408 + yfcG HEPCGN_21970 0.34 +1.2 3,149,409 - yfcG HEPCGN_21970 0.34 +1.0 3,149,482 + yfcG HEPCGN_21970 0.45 +0.4 3,149,534 + yfcG HEPCGN_21970 0.53 -1.7 3,149,543 + yfcG HEPCGN_21970 0.55 -0.8 3,149,544 - yfcG HEPCGN_21970 0.55 +0.3 3,149,823 + +1.1 3,149,854 - +0.0 3,149,875 - +0.0 3,149,877 - -0.2 3,149,934 + -0.8 3,149,970 + -0.3 3,149,970 + +1.6 3,149,970 + -0.2 3,149,970 + -1.0 3,149,971 - -0.8 3,149,977 + -0.1 3,149,978 - -0.6 3,150,023 + -0.2 3,150,023 + +0.3 3,150,023 + +0.0 3,150,073 + yfcF HEPCGN_21975 0.16 -1.2 3,150,156 + yfcF HEPCGN_21975 0.29 -0.6 3,150,241 + yfcF HEPCGN_21975 0.42 -0.8 3,150,242 - yfcF HEPCGN_21975 0.42 -0.4 3,150,476 - yfcF HEPCGN_21975 0.78 -0.2 3,150,510 + yfcF HEPCGN_21975 0.83 -0.4 3,150,511 - yfcF HEPCGN_21975 0.84 +0.4 3,150,641 + -0.5 3,150,641 + +0.1 3,150,809 - yfcE HEPCGN_21980 0.25 +0.5
Or see this region's nucleotide sequence