Experiment: K20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiC and thiE overlap by 1 nucleotides thiE and thiF overlap by 8 nucleotides thiF and thiF overlap by 17 nucleotides thiF and thiG are separated by 1 nucleotides thiG and thiH overlap by 4 nucleotides
HEPCGN_12010: thiC - phosphomethylpyrimidine synthase ThiC, at 1,141,535 to 1,143,430
thiC
HEPCGN_12015: thiE - thiamine phosphate synthase, at 1,143,430 to 1,144,065
thiE
HEPCGN_12020: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,144,058 to 1,144,813
thiF
HEPCGN_12025: thiF - thiazole biosynthesis adenylyltransferase ThiF, at 1,144,797 to 1,144,997
thiF
HEPCGN_12030: thiG - thiazole synthase, at 1,144,999 to 1,145,769
thiG
HEPCGN_12035: thiH - 2-iminoacetate synthase ThiH, at 1,145,766 to 1,146,899
thiH
Position (kb)
1144
1145 Strain fitness (log2 ratio)
-2
-1
0
1 at 1143.077 kb on - strand, within thiC at 1143.084 kb on - strand, within thiC at 1143.193 kb on + strand, within thiC at 1143.324 kb on + strand at 1143.324 kb on + strand at 1143.325 kb on - strand at 1143.325 kb on - strand at 1143.332 kb on + strand at 1143.332 kb on + strand at 1143.371 kb on - strand at 1143.456 kb on - strand at 1143.490 kb on - strand at 1143.494 kb on + strand, within thiE at 1143.494 kb on + strand, within thiE at 1143.495 kb on - strand, within thiE at 1143.670 kb on + strand, within thiE at 1143.671 kb on - strand, within thiE at 1143.802 kb on + strand, within thiE at 1143.802 kb on + strand, within thiE at 1143.802 kb on + strand, within thiE at 1143.803 kb on - strand, within thiE at 1143.803 kb on - strand, within thiE at 1143.804 kb on + strand, within thiE at 1143.804 kb on + strand, within thiE at 1143.805 kb on - strand, within thiE at 1143.996 kb on + strand, within thiE at 1143.997 kb on - strand, within thiE at 1143.997 kb on - strand, within thiE at 1144.082 kb on + strand at 1144.084 kb on + strand at 1144.085 kb on - strand at 1144.085 kb on - strand at 1144.193 kb on + strand, within thiF at 1144.194 kb on - strand, within thiF at 1144.224 kb on + strand, within thiF at 1144.252 kb on - strand, within thiF at 1144.279 kb on + strand, within thiF at 1144.365 kb on + strand, within thiF at 1144.365 kb on + strand, within thiF at 1144.365 kb on + strand, within thiF at 1144.366 kb on - strand, within thiF at 1144.392 kb on + strand, within thiF at 1144.392 kb on + strand, within thiF at 1144.495 kb on + strand, within thiF at 1144.496 kb on - strand, within thiF at 1144.536 kb on + strand, within thiF at 1144.646 kb on - strand, within thiF at 1144.680 kb on + strand, within thiF at 1144.691 kb on - strand, within thiF at 1144.693 kb on - strand, within thiF at 1144.693 kb on - strand, within thiF at 1144.944 kb on - strand, within thiF at 1144.944 kb on - strand, within thiF at 1145.003 kb on + strand at 1145.007 kb on + strand at 1145.008 kb on - strand at 1145.203 kb on - strand, within thiG at 1145.285 kb on + strand, within thiG at 1145.441 kb on + strand, within thiG at 1145.604 kb on - strand, within thiG
Per-strain Table
Position Strand Gene LocusTag Fraction K20 remove 1,143,077 - thiC HEPCGN_12010 0.81 -2.1 1,143,084 - thiC HEPCGN_12010 0.82 -0.2 1,143,193 + thiC HEPCGN_12010 0.87 +0.9 1,143,324 + +0.5 1,143,324 + -0.4 1,143,325 - +1.5 1,143,325 - -0.2 1,143,332 + -0.1 1,143,332 + -0.3 1,143,371 - +0.3 1,143,456 - -0.6 1,143,490 - -0.2 1,143,494 + thiE HEPCGN_12015 0.10 +1.7 1,143,494 + thiE HEPCGN_12015 0.10 +1.2 1,143,495 - thiE HEPCGN_12015 0.10 -1.5 1,143,670 + thiE HEPCGN_12015 0.38 +0.9 1,143,671 - thiE HEPCGN_12015 0.38 +0.1 1,143,802 + thiE HEPCGN_12015 0.58 -0.4 1,143,802 + thiE HEPCGN_12015 0.58 -0.0 1,143,802 + thiE HEPCGN_12015 0.58 -0.3 1,143,803 - thiE HEPCGN_12015 0.59 -0.0 1,143,803 - thiE HEPCGN_12015 0.59 +0.0 1,143,804 + thiE HEPCGN_12015 0.59 +0.9 1,143,804 + thiE HEPCGN_12015 0.59 +0.5 1,143,805 - thiE HEPCGN_12015 0.59 +0.2 1,143,996 + thiE HEPCGN_12015 0.89 -1.1 1,143,997 - thiE HEPCGN_12015 0.89 +0.0 1,143,997 - thiE HEPCGN_12015 0.89 -0.2 1,144,082 + +0.1 1,144,084 + -0.5 1,144,085 - -0.4 1,144,085 - +1.0 1,144,193 + thiF HEPCGN_12020 0.18 +0.1 1,144,194 - thiF HEPCGN_12020 0.18 +1.1 1,144,224 + thiF HEPCGN_12020 0.22 +0.6 1,144,252 - thiF HEPCGN_12020 0.26 +0.2 1,144,279 + thiF HEPCGN_12020 0.29 +0.2 1,144,365 + thiF HEPCGN_12020 0.41 +0.6 1,144,365 + thiF HEPCGN_12020 0.41 +0.2 1,144,365 + thiF HEPCGN_12020 0.41 -0.3 1,144,366 - thiF HEPCGN_12020 0.41 -0.3 1,144,392 + thiF HEPCGN_12020 0.44 -0.1 1,144,392 + thiF HEPCGN_12020 0.44 -0.1 1,144,495 + thiF HEPCGN_12020 0.58 -0.9 1,144,496 - thiF HEPCGN_12020 0.58 -0.2 1,144,536 + thiF HEPCGN_12020 0.63 +0.5 1,144,646 - thiF HEPCGN_12020 0.78 -0.1 1,144,680 + thiF HEPCGN_12020 0.82 -2.6 1,144,691 - thiF HEPCGN_12020 0.84 -0.7 1,144,693 - thiF HEPCGN_12020 0.84 +0.7 1,144,693 - thiF HEPCGN_12020 0.84 +0.5 1,144,944 - thiF HEPCGN_12025 0.73 -0.5 1,144,944 - thiF HEPCGN_12025 0.73 -0.7 1,145,003 + -0.3 1,145,007 + -0.5 1,145,008 - +0.2 1,145,203 - thiG HEPCGN_12030 0.26 -0.1 1,145,285 + thiG HEPCGN_12030 0.37 -0.9 1,145,441 + thiG HEPCGN_12030 0.57 +0.0 1,145,604 - thiG HEPCGN_12030 0.78 -0.3
Or see this region's nucleotide sequence