Experiment: K20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpE and arfB are separated by 13 nucleotides arfB and yaeQ overlap by 4 nucleotides yaeQ and yaeP are separated by 165 nucleotides yaeP and rof overlap by 14 nucleotides rof and tilS are separated by 48 nucleotides
HEPCGN_08045: nlpE - envelope stress response activation lipoprotein NlpE, at 344,213 to 344,923
nlpE
HEPCGN_08050: arfB - aminoacyl-tRNA hydrolase, at 344,937 to 345,359
arfB
HEPCGN_08055: yaeQ - Uncharacterized protein YaeQ, at 345,356 to 345,901
yaeQ
HEPCGN_08060: yaeP - YaeP family protein, at 346,067 to 346,267
yaeP
HEPCGN_08065: rof - Rho-binding antiterminator, at 346,254 to 346,514
rof
HEPCGN_08070: tilS - tRNA lysidine(34) synthetase TilS, at 346,563 to 347,861
tilS
Position (kb) 345
346 Strain fitness (log2 ratio) -1
0
1
2 at 344.466 kb on + strand, within nlpE at 344.467 kb on - strand, within nlpE at 344.499 kb on - strand, within nlpE at 344.499 kb on - strand, within nlpE at 344.526 kb on + strand, within nlpE at 344.598 kb on + strand, within nlpE at 344.627 kb on + strand, within nlpE at 344.627 kb on + strand, within nlpE at 344.628 kb on - strand, within nlpE at 344.656 kb on + strand, within nlpE at 344.657 kb on - strand, within nlpE at 344.661 kb on + strand, within nlpE at 344.860 kb on + strand at 344.861 kb on - strand at 344.876 kb on - strand at 345.038 kb on + strand, within arfB at 345.208 kb on + strand, within arfB at 345.208 kb on + strand, within arfB at 345.209 kb on - strand, within arfB at 345.266 kb on + strand, within arfB at 345.266 kb on + strand, within arfB at 345.398 kb on - strand at 345.404 kb on - strand at 345.404 kb on - strand at 345.421 kb on + strand, within yaeQ at 345.421 kb on + strand, within yaeQ at 345.578 kb on - strand, within yaeQ at 345.580 kb on + strand, within yaeQ at 345.580 kb on + strand, within yaeQ at 345.581 kb on - strand, within yaeQ at 345.581 kb on - strand, within yaeQ at 345.581 kb on - strand, within yaeQ at 345.625 kb on + strand, within yaeQ at 345.671 kb on - strand, within yaeQ at 345.753 kb on + strand, within yaeQ at 345.753 kb on + strand, within yaeQ at 345.754 kb on - strand, within yaeQ at 345.878 kb on - strand at 345.880 kb on - strand at 345.942 kb on + strand at 345.963 kb on + strand at 345.985 kb on + strand at 345.986 kb on - strand at 345.986 kb on - strand at 346.016 kb on + strand at 346.041 kb on - strand at 346.041 kb on - strand at 346.090 kb on - strand, within yaeP at 346.131 kb on - strand, within yaeP at 346.268 kb on + strand at 346.268 kb on + strand at 346.272 kb on + strand at 346.273 kb on - strand at 346.273 kb on - strand at 346.296 kb on + strand, within rof at 346.296 kb on + strand, within rof at 346.330 kb on + strand, within rof at 346.330 kb on + strand, within rof at 346.330 kb on + strand, within rof at 346.330 kb on + strand, within rof at 346.331 kb on - strand, within rof at 346.331 kb on - strand, within rof at 346.434 kb on - strand, within rof at 346.568 kb on + strand at 346.568 kb on + strand at 346.569 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K20 remove 344,466 + nlpE HEPCGN_08045 0.36 +2.1 344,467 - nlpE HEPCGN_08045 0.36 +0.4 344,499 - nlpE HEPCGN_08045 0.40 -0.9 344,499 - nlpE HEPCGN_08045 0.40 -1.6 344,526 + nlpE HEPCGN_08045 0.44 -0.3 344,598 + nlpE HEPCGN_08045 0.54 -0.1 344,627 + nlpE HEPCGN_08045 0.58 -0.3 344,627 + nlpE HEPCGN_08045 0.58 +0.5 344,628 - nlpE HEPCGN_08045 0.58 -0.6 344,656 + nlpE HEPCGN_08045 0.62 -0.2 344,657 - nlpE HEPCGN_08045 0.62 -0.4 344,661 + nlpE HEPCGN_08045 0.63 +0.4 344,860 + +0.3 344,861 - -0.3 344,876 - +0.1 345,038 + arfB HEPCGN_08050 0.24 -1.4 345,208 + arfB HEPCGN_08050 0.64 +0.3 345,208 + arfB HEPCGN_08050 0.64 +0.5 345,209 - arfB HEPCGN_08050 0.64 +0.5 345,266 + arfB HEPCGN_08050 0.78 -0.3 345,266 + arfB HEPCGN_08050 0.78 -0.0 345,398 - -0.1 345,404 - +0.3 345,404 - -0.2 345,421 + yaeQ HEPCGN_08055 0.12 -0.1 345,421 + yaeQ HEPCGN_08055 0.12 +0.9 345,578 - yaeQ HEPCGN_08055 0.41 +0.7 345,580 + yaeQ HEPCGN_08055 0.41 +0.6 345,580 + yaeQ HEPCGN_08055 0.41 +0.6 345,581 - yaeQ HEPCGN_08055 0.41 +0.6 345,581 - yaeQ HEPCGN_08055 0.41 +0.1 345,581 - yaeQ HEPCGN_08055 0.41 -0.3 345,625 + yaeQ HEPCGN_08055 0.49 +0.4 345,671 - yaeQ HEPCGN_08055 0.58 -0.1 345,753 + yaeQ HEPCGN_08055 0.73 -0.7 345,753 + yaeQ HEPCGN_08055 0.73 -0.1 345,754 - yaeQ HEPCGN_08055 0.73 -0.5 345,878 - -0.7 345,880 - -0.8 345,942 + -0.3 345,963 + -0.4 345,985 + +0.3 345,986 - -0.6 345,986 - -1.7 346,016 + +0.1 346,041 - -0.4 346,041 - -0.7 346,090 - yaeP HEPCGN_08060 0.11 -0.6 346,131 - yaeP HEPCGN_08060 0.32 -0.1 346,268 + -0.6 346,268 + +0.0 346,272 + -1.0 346,273 - +0.3 346,273 - -0.4 346,296 + rof HEPCGN_08065 0.16 +0.0 346,296 + rof HEPCGN_08065 0.16 +0.8 346,330 + rof HEPCGN_08065 0.29 +1.0 346,330 + rof HEPCGN_08065 0.29 +0.6 346,330 + rof HEPCGN_08065 0.29 +1.1 346,330 + rof HEPCGN_08065 0.29 +0.1 346,331 - rof HEPCGN_08065 0.30 +0.0 346,331 - rof HEPCGN_08065 0.30 -1.3 346,434 - rof HEPCGN_08065 0.69 +0.0 346,568 + -0.3 346,568 + +1.1 346,569 - +0.0
Or see this region's nucleotide sequence