Strain Fitness in Escherichia coli ECOR38 around HEPCGN_03110

Experiment: K20

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfbB and rfbD overlap by 1 nucleotidesrfbD and rfbA are separated by 58 nucleotidesrfbA and rfbC are separated by 4 nucleotides HEPCGN_03105: rfbB - dTDP-glucose 4,6-dehydratase, at 532,570 to 533,655 rfbB HEPCGN_03110: rfbD - dTDP-4-dehydrorhamnose reductase, at 533,655 to 534,554 rfbD HEPCGN_03115: rfbA - glucose-1-phosphate thymidylyltransferase RfbA, at 534,613 to 535,491 rfbA HEPCGN_03120: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 535,496 to 536,041 rfbC Position (kb) 533 534 535Strain fitness (log2 ratio) -1 0 1 2 3at 534.118 kb on - strand, within rfbDat 534.630 kb on + strandat 534.744 kb on + strand, within rfbAat 535.424 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K20
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534,118 - rfbD HEPCGN_03110 0.51 +2.5
534,630 + +3.0
534,744 + rfbA HEPCGN_03115 0.15 +2.4
535,424 + +1.7

Or see this region's nucleotide sequence