Experiment: K20
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Leu_trna and Met_trna are separated by 8 nucleotides Met_trna and HEPCGN_01045 are separated by 331 nucleotides HEPCGN_01045 and asnB are separated by 41 nucleotides asnB and nagD are separated by 256 nucleotides
HEPCGN_01035: Leu_trna - tRNA-Leu(tag), at 131,386 to 131,470
_trna
HEPCGN_01040: Met_trna - tRNA-Met(cat), at 131,479 to 131,555
_trna
HEPCGN_01045: HEPCGN_01045 - hypothetical protein, at 131,887 to 132,324
_01045
HEPCGN_01050: asnB - asparagine synthase B, at 132,366 to 134,030
asnB
HEPCGN_01055: nagD - ribonucleotide monophosphatase NagD, at 134,287 to 135,039
nagD
Position (kb) 132
133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2 at 131.368 kb on - strand at 131.688 kb on - strand at 131.688 kb on - strand at 131.688 kb on - strand at 131.689 kb on + strand at 131.700 kb on + strand at 131.730 kb on + strand at 131.730 kb on + strand at 131.883 kb on + strand at 131.886 kb on + strand at 131.886 kb on + strand at 131.886 kb on + strand at 131.887 kb on - strand at 131.887 kb on - strand at 131.924 kb on + strand at 131.924 kb on + strand at 131.925 kb on - strand at 131.925 kb on - strand at 131.925 kb on - strand at 131.928 kb on + strand at 131.928 kb on + strand at 131.974 kb on + strand, within HEPCGN_01045 at 131.975 kb on - strand, within HEPCGN_01045 at 131.975 kb on - strand, within HEPCGN_01045 at 132.043 kb on - strand, within HEPCGN_01045 at 132.106 kb on - strand, within HEPCGN_01045 at 132.119 kb on + strand, within HEPCGN_01045 at 132.119 kb on + strand, within HEPCGN_01045 at 132.142 kb on - strand, within HEPCGN_01045 at 132.174 kb on - strand, within HEPCGN_01045 at 132.174 kb on - strand, within HEPCGN_01045 at 132.177 kb on - strand, within HEPCGN_01045 at 132.208 kb on - strand, within HEPCGN_01045 at 132.208 kb on - strand, within HEPCGN_01045 at 132.242 kb on - strand, within HEPCGN_01045 at 132.298 kb on + strand at 132.299 kb on - strand at 132.322 kb on + strand at 132.323 kb on - strand at 132.367 kb on + strand at 132.367 kb on + strand at 132.372 kb on - strand at 132.372 kb on - strand at 132.374 kb on - strand at 132.544 kb on + strand, within asnB at 132.771 kb on - strand, within asnB at 132.880 kb on + strand, within asnB at 132.880 kb on + strand, within asnB at 132.881 kb on - strand, within asnB at 132.881 kb on - strand, within asnB at 132.881 kb on - strand, within asnB at 132.881 kb on - strand, within asnB at 132.881 kb on - strand, within asnB at 132.881 kb on - strand, within asnB at 132.881 kb on - strand, within asnB at 132.884 kb on + strand, within asnB at 132.884 kb on + strand, within asnB at 132.885 kb on - strand, within asnB at 132.885 kb on - strand, within asnB at 132.952 kb on + strand, within asnB at 132.953 kb on - strand, within asnB at 133.036 kb on - strand, within asnB at 133.036 kb on - strand, within asnB at 133.075 kb on + strand, within asnB at 133.090 kb on + strand, within asnB at 133.126 kb on - strand, within asnB at 133.213 kb on - strand, within asnB at 133.283 kb on - strand, within asnB at 133.330 kb on - strand, within asnB at 133.342 kb on + strand, within asnB at 133.342 kb on + strand, within asnB at 133.419 kb on - strand, within asnB at 133.436 kb on - strand, within asnB at 133.492 kb on + strand, within asnB at 133.492 kb on + strand, within asnB at 133.493 kb on - strand, within asnB at 133.493 kb on - strand, within asnB at 133.493 kb on - strand, within asnB at 133.493 kb on - strand, within asnB at 133.515 kb on - strand, within asnB at 133.562 kb on - strand, within asnB at 133.591 kb on + strand, within asnB at 133.591 kb on + strand, within asnB at 133.591 kb on + strand, within asnB at 133.591 kb on + strand, within asnB at 133.592 kb on - strand, within asnB at 133.592 kb on - strand, within asnB at 133.592 kb on - strand, within asnB at 133.711 kb on + strand, within asnB at 133.769 kb on - strand, within asnB at 133.795 kb on + strand, within asnB at 133.796 kb on - strand, within asnB at 133.818 kb on + strand, within asnB at 133.840 kb on + strand, within asnB at 133.841 kb on - strand, within asnB at 133.841 kb on - strand, within asnB at 133.841 kb on - strand, within asnB at 133.841 kb on - strand, within asnB at 133.915 kb on + strand at 133.916 kb on - strand at 134.031 kb on - strand at 134.343 kb on + strand at 134.422 kb on - strand, within nagD at 134.522 kb on + strand, within nagD at 134.522 kb on + strand, within nagD at 134.523 kb on - strand, within nagD at 134.523 kb on - strand, within nagD at 134.523 kb on - strand, within nagD at 134.533 kb on - strand, within nagD at 134.722 kb on - strand, within nagD at 134.741 kb on + strand, within nagD at 134.741 kb on + strand, within nagD at 134.741 kb on + strand, within nagD at 134.742 kb on - strand, within nagD at 134.778 kb on - strand, within nagD at 134.778 kb on - strand, within nagD at 134.834 kb on + strand, within nagD at 134.834 kb on + strand, within nagD at 134.835 kb on - strand, within nagD at 134.909 kb on + strand, within nagD at 134.909 kb on + strand, within nagD at 134.940 kb on + strand, within nagD at 135.030 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K20 remove 131,368 - -0.5 131,688 - +1.0 131,688 - -1.1 131,688 - -1.2 131,689 + -1.3 131,700 + -2.0 131,730 + -1.2 131,730 + -0.7 131,883 + -0.6 131,886 + -0.5 131,886 + +0.8 131,886 + -0.2 131,887 - -0.2 131,887 - +0.1 131,924 + -0.1 131,924 + -0.2 131,925 - -0.7 131,925 - +0.2 131,925 - -0.1 131,928 + -0.7 131,928 + +0.1 131,974 + HEPCGN_01045 0.20 -0.1 131,975 - HEPCGN_01045 0.20 +0.8 131,975 - HEPCGN_01045 0.20 -0.7 132,043 - HEPCGN_01045 0.36 -0.7 132,106 - HEPCGN_01045 0.50 -0.4 132,119 + HEPCGN_01045 0.53 +0.2 132,119 + HEPCGN_01045 0.53 -0.2 132,142 - HEPCGN_01045 0.58 -0.3 132,174 - HEPCGN_01045 0.66 -0.8 132,174 - HEPCGN_01045 0.66 -0.3 132,177 - HEPCGN_01045 0.66 +0.3 132,208 - HEPCGN_01045 0.73 +0.7 132,208 - HEPCGN_01045 0.73 +0.5 132,242 - HEPCGN_01045 0.81 -0.3 132,298 + -1.7 132,299 - +0.8 132,322 + +0.3 132,323 - +0.7 132,367 + +0.1 132,367 + +0.6 132,372 - +0.7 132,372 - -0.8 132,374 - -0.2 132,544 + asnB HEPCGN_01050 0.11 -0.1 132,771 - asnB HEPCGN_01050 0.24 +2.2 132,880 + asnB HEPCGN_01050 0.31 -1.0 132,880 + asnB HEPCGN_01050 0.31 +0.2 132,881 - asnB HEPCGN_01050 0.31 -0.0 132,881 - asnB HEPCGN_01050 0.31 -0.8 132,881 - asnB HEPCGN_01050 0.31 +0.8 132,881 - asnB HEPCGN_01050 0.31 -0.5 132,881 - asnB HEPCGN_01050 0.31 -1.2 132,881 - asnB HEPCGN_01050 0.31 -0.6 132,881 - asnB HEPCGN_01050 0.31 +0.8 132,884 + asnB HEPCGN_01050 0.31 -0.8 132,884 + asnB HEPCGN_01050 0.31 +0.7 132,885 - asnB HEPCGN_01050 0.31 -1.4 132,885 - asnB HEPCGN_01050 0.31 -0.7 132,952 + asnB HEPCGN_01050 0.35 +0.3 132,953 - asnB HEPCGN_01050 0.35 -0.1 133,036 - asnB HEPCGN_01050 0.40 +2.2 133,036 - asnB HEPCGN_01050 0.40 -0.5 133,075 + asnB HEPCGN_01050 0.43 +0.8 133,090 + asnB HEPCGN_01050 0.43 +0.8 133,126 - asnB HEPCGN_01050 0.46 +0.4 133,213 - asnB HEPCGN_01050 0.51 +0.0 133,283 - asnB HEPCGN_01050 0.55 +0.4 133,330 - asnB HEPCGN_01050 0.58 -0.2 133,342 + asnB HEPCGN_01050 0.59 -2.4 133,342 + asnB HEPCGN_01050 0.59 -0.5 133,419 - asnB HEPCGN_01050 0.63 -1.2 133,436 - asnB HEPCGN_01050 0.64 -0.5 133,492 + asnB HEPCGN_01050 0.68 +0.4 133,492 + asnB HEPCGN_01050 0.68 -0.8 133,493 - asnB HEPCGN_01050 0.68 -0.5 133,493 - asnB HEPCGN_01050 0.68 +0.4 133,493 - asnB HEPCGN_01050 0.68 +1.8 133,493 - asnB HEPCGN_01050 0.68 -0.6 133,515 - asnB HEPCGN_01050 0.69 -0.7 133,562 - asnB HEPCGN_01050 0.72 +0.4 133,591 + asnB HEPCGN_01050 0.74 +0.8 133,591 + asnB HEPCGN_01050 0.74 +1.3 133,591 + asnB HEPCGN_01050 0.74 -0.1 133,591 + asnB HEPCGN_01050 0.74 -0.9 133,592 - asnB HEPCGN_01050 0.74 -1.6 133,592 - asnB HEPCGN_01050 0.74 -0.3 133,592 - asnB HEPCGN_01050 0.74 +0.4 133,711 + asnB HEPCGN_01050 0.81 -0.1 133,769 - asnB HEPCGN_01050 0.84 +0.6 133,795 + asnB HEPCGN_01050 0.86 -1.1 133,796 - asnB HEPCGN_01050 0.86 +0.9 133,818 + asnB HEPCGN_01050 0.87 -0.9 133,840 + asnB HEPCGN_01050 0.89 -1.4 133,841 - asnB HEPCGN_01050 0.89 +0.0 133,841 - asnB HEPCGN_01050 0.89 -0.2 133,841 - asnB HEPCGN_01050 0.89 +0.2 133,841 - asnB HEPCGN_01050 0.89 +0.2 133,915 + +0.9 133,916 - -0.0 134,031 - +0.1 134,343 + +0.9 134,422 - nagD HEPCGN_01055 0.18 -0.1 134,522 + nagD HEPCGN_01055 0.31 +0.6 134,522 + nagD HEPCGN_01055 0.31 +0.1 134,523 - nagD HEPCGN_01055 0.31 -0.4 134,523 - nagD HEPCGN_01055 0.31 +0.2 134,523 - nagD HEPCGN_01055 0.31 -0.0 134,533 - nagD HEPCGN_01055 0.33 -0.2 134,722 - nagD HEPCGN_01055 0.58 -0.6 134,741 + nagD HEPCGN_01055 0.60 -1.1 134,741 + nagD HEPCGN_01055 0.60 +0.6 134,741 + nagD HEPCGN_01055 0.60 -0.9 134,742 - nagD HEPCGN_01055 0.60 +1.5 134,778 - nagD HEPCGN_01055 0.65 -0.6 134,778 - nagD HEPCGN_01055 0.65 -0.3 134,834 + nagD HEPCGN_01055 0.73 -1.8 134,834 + nagD HEPCGN_01055 0.73 +0.2 134,835 - nagD HEPCGN_01055 0.73 -0.8 134,909 + nagD HEPCGN_01055 0.83 -0.8 134,909 + nagD HEPCGN_01055 0.83 +1.2 134,940 + nagD HEPCGN_01055 0.87 +1.2 135,030 + +0.4
Or see this region's nucleotide sequence