Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF990 and GFF991 are separated by 152 nucleotides GFF991 and GFF992 are separated by 21 nucleotides
GFF990 - LSU rRNA pseudouridine(2605) synthase (EC 5.4.99.22), at 308,595 to 310,394
GFF990
GFF991 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 310,547 to 310,972
GFF991
GFF992 - 23S rRNA (adenine(2503)-C(2))-methyltransferase @ tRNA (adenine(37)-C(2))-methyltransferase (EC 2.1.1.192), at 310,994 to 312,142
GFF992
Position (kb) 310
311 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 309.583 kb on - strand, within GFF990 at 309.583 kb on - strand, within GFF990 at 310.266 kb on + strand at 310.266 kb on + strand at 310.266 kb on + strand at 310.266 kb on + strand at 310.266 kb on + strand at 310.266 kb on + strand at 310.267 kb on - strand at 310.267 kb on - strand at 310.267 kb on - strand at 310.267 kb on - strand at 310.267 kb on - strand at 310.480 kb on + strand at 310.480 kb on + strand at 310.961 kb on + strand at 310.961 kb on + strand at 310.961 kb on + strand at 310.962 kb on - strand at 310.978 kb on + strand at 310.978 kb on + strand at 310.978 kb on + strand at 310.978 kb on + strand at 310.978 kb on + strand at 310.978 kb on + strand at 310.978 kb on + strand at 310.979 kb on - strand at 310.979 kb on - strand at 310.979 kb on - strand at 310.979 kb on - strand at 311.274 kb on + strand, within GFF992 at 311.274 kb on + strand, within GFF992 at 311.274 kb on + strand, within GFF992 at 311.275 kb on - strand, within GFF992 at 311.275 kb on - strand, within GFF992 at 311.275 kb on - strand, within GFF992 at 311.275 kb on - strand, within GFF992 at 311.465 kb on + strand, within GFF992 at 311.507 kb on + strand, within GFF992 at 311.507 kb on + strand, within GFF992 at 311.507 kb on + strand, within GFF992 at 311.507 kb on + strand, within GFF992 at 311.555 kb on + strand, within GFF992 at 311.708 kb on + strand, within GFF992 at 311.708 kb on + strand, within GFF992 at 311.708 kb on + strand, within GFF992 at 311.708 kb on + strand, within GFF992 at 311.709 kb on - strand, within GFF992 at 311.744 kb on + strand, within GFF992 at 311.744 kb on + strand, within GFF992 at 311.744 kb on + strand, within GFF992 at 311.783 kb on + strand, within GFF992 at 311.783 kb on + strand, within GFF992 at 311.783 kb on + strand, within GFF992 at 311.784 kb on - strand, within GFF992 at 311.784 kb on - strand, within GFF992 at 311.784 kb on - strand, within GFF992 at 311.784 kb on - strand, within GFF992
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 309,583 - GFF990 0.55 +2.7 309,583 - GFF990 0.55 +0.1 310,266 + +0.7 310,266 + -0.5 310,266 + +1.2 310,266 + +0.6 310,266 + -0.1 310,266 + +0.4 310,267 - +1.2 310,267 - +0.0 310,267 - +0.1 310,267 - +0.2 310,267 - -0.2 310,480 + -0.7 310,480 + +1.4 310,961 + -1.5 310,961 + +0.3 310,961 + -0.9 310,962 - -0.4 310,978 + -0.5 310,978 + -1.1 310,978 + -0.2 310,978 + -0.1 310,978 + -2.2 310,978 + +0.1 310,978 + -1.2 310,979 - -0.1 310,979 - -1.3 310,979 - +0.2 310,979 - -1.5 311,274 + GFF992 0.24 -0.6 311,274 + GFF992 0.24 -1.0 311,274 + GFF992 0.24 -0.6 311,275 - GFF992 0.24 -0.7 311,275 - GFF992 0.24 -0.6 311,275 - GFF992 0.24 -0.9 311,275 - GFF992 0.24 +0.3 311,465 + GFF992 0.41 -0.2 311,507 + GFF992 0.45 -1.6 311,507 + GFF992 0.45 -0.9 311,507 + GFF992 0.45 +0.2 311,507 + GFF992 0.45 -1.0 311,555 + GFF992 0.49 -1.1 311,708 + GFF992 0.62 -0.5 311,708 + GFF992 0.62 -0.4 311,708 + GFF992 0.62 -0.5 311,708 + GFF992 0.62 +0.1 311,709 - GFF992 0.62 -1.2 311,744 + GFF992 0.65 +0.4 311,744 + GFF992 0.65 +0.8 311,744 + GFF992 0.65 +0.5 311,783 + GFF992 0.69 +0.4 311,783 + GFF992 0.69 -1.2 311,783 + GFF992 0.69 -3.3 311,784 - GFF992 0.69 +0.4 311,784 - GFF992 0.69 +0.8 311,784 - GFF992 0.69 -1.2 311,784 - GFF992 0.69 -0.3
Or see this region's nucleotide sequence