Strain Fitness in Variovorax sp. SCN45 around GFF981

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF980 and GFF981 are separated by 18 nucleotidesGFF981 and GFF982 are separated by 4 nucleotides GFF980 - Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61), at 297,992 to 299,263 GFF980 GFF981 - Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4), at 299,282 to 300,712 GFF981 GFF982 - Cell division protein ZapE, at 300,717 to 301,817 GFF982 Position (kb) 299 300 301Strain fitness (log2 ratio) -2 -1 0 1 2at 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.738 kb on + strandat 300.739 kb on - strandat 300.739 kb on - strandat 300.739 kb on - strandat 300.739 kb on - strandat 300.834 kb on + strand, within GFF982at 300.834 kb on + strand, within GFF982at 300.834 kb on + strand, within GFF982at 300.834 kb on + strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.835 kb on - strand, within GFF982at 300.900 kb on + strand, within GFF982at 300.900 kb on + strand, within GFF982at 300.900 kb on + strand, within GFF982at 300.900 kb on + strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.901 kb on - strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.906 kb on + strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 300.907 kb on - strand, within GFF982at 301.086 kb on + strand, within GFF982at 301.086 kb on + strand, within GFF982at 301.086 kb on + strand, within GFF982at 301.087 kb on - strand, within GFF982at 301.087 kb on - strand, within GFF982at 301.087 kb on - strand, within GFF982at 301.222 kb on - strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.347 kb on + strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.348 kb on - strand, within GFF982at 301.440 kb on - strand, within GFF982at 301.440 kb on - strand, within GFF982at 301.536 kb on + strand, within GFF982at 301.537 kb on - strand, within GFF982at 301.647 kb on + strand, within GFF982at 301.648 kb on - strand, within GFF982at 301.648 kb on - strand, within GFF982at 301.648 kb on - strand, within GFF982at 301.648 kb on - strand, within GFF982at 301.701 kb on + strand, within GFF982at 301.701 kb on + strand, within GFF982at 301.701 kb on + strand, within GFF982at 301.701 kb on + strand, within GFF982at 301.701 kb on + strand, within GFF982at 301.701 kb on + strand, within GFF982at 301.701 kb on - strand, within GFF982at 301.702 kb on - strand, within GFF982at 301.702 kb on - strand, within GFF982at 301.702 kb on - strand, within GFF982at 301.702 kb on - strand, within GFF982at 301.702 kb on - strand, within GFF982at 301.702 kb on - strand, within GFF982at 301.702 kb on - strand, within GFF982

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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300,738 + -0.8
300,738 + +0.8
300,738 + -0.5
300,738 + +0.9
300,738 + +2.1
300,738 + +0.1
300,738 + +1.4
300,738 + +0.9
300,738 + +0.7
300,738 + -0.6
300,739 - +0.3
300,739 - -0.1
300,739 - +0.1
300,739 - -2.2
300,834 + GFF982 0.11 +1.0
300,834 + GFF982 0.11 -0.5
300,834 + GFF982 0.11 +0.7
300,834 + GFF982 0.11 -0.8
300,835 - GFF982 0.11 +0.0
300,835 - GFF982 0.11 +0.2
300,835 - GFF982 0.11 +1.4
300,835 - GFF982 0.11 +0.8
300,835 - GFF982 0.11 -0.0
300,835 - GFF982 0.11 +0.4
300,835 - GFF982 0.11 -0.5
300,835 - GFF982 0.11 -0.5
300,900 + GFF982 0.17 +1.2
300,900 + GFF982 0.17 +0.2
300,900 + GFF982 0.17 +0.5
300,900 + GFF982 0.17 -0.2
300,901 - GFF982 0.17 -0.8
300,901 - GFF982 0.17 +2.2
300,901 - GFF982 0.17 +1.4
300,901 - GFF982 0.17 -0.4
300,901 - GFF982 0.17 +1.0
300,901 - GFF982 0.17 +0.6
300,901 - GFF982 0.17 +0.1
300,901 - GFF982 0.17 +0.0
300,906 + GFF982 0.17 -1.2
300,906 + GFF982 0.17 +0.5
300,906 + GFF982 0.17 +2.0
300,906 + GFF982 0.17 +0.4
300,906 + GFF982 0.17 +1.4
300,906 + GFF982 0.17 +0.2
300,906 + GFF982 0.17 -1.2
300,906 + GFF982 0.17 -0.2
300,906 + GFF982 0.17 +0.4
300,906 + GFF982 0.17 +0.2
300,906 + GFF982 0.17 +0.4
300,906 + GFF982 0.17 +0.0
300,906 + GFF982 0.17 -0.1
300,906 + GFF982 0.17 +0.8
300,906 + GFF982 0.17 +0.9
300,906 + GFF982 0.17 +0.8
300,906 + GFF982 0.17 +1.0
300,906 + GFF982 0.17 +1.7
300,906 + GFF982 0.17 +0.7
300,906 + GFF982 0.17 -0.2
300,907 - GFF982 0.17 +0.9
300,907 - GFF982 0.17 +1.1
300,907 - GFF982 0.17 +0.6
300,907 - GFF982 0.17 +0.1
300,907 - GFF982 0.17 +0.5
300,907 - GFF982 0.17 +0.8
300,907 - GFF982 0.17 -0.4
300,907 - GFF982 0.17 +0.1
300,907 - GFF982 0.17 +1.3
300,907 - GFF982 0.17 +0.8
300,907 - GFF982 0.17 -1.1
300,907 - GFF982 0.17 +0.0
300,907 - GFF982 0.17 +0.5
300,907 - GFF982 0.17 +0.1
300,907 - GFF982 0.17 +0.6
300,907 - GFF982 0.17 +1.4
300,907 - GFF982 0.17 +0.9
300,907 - GFF982 0.17 -0.5
300,907 - GFF982 0.17 +0.5
300,907 - GFF982 0.17 -0.4
300,907 - GFF982 0.17 +0.0
300,907 - GFF982 0.17 -0.1
300,907 - GFF982 0.17 +1.4
300,907 - GFF982 0.17 +0.4
300,907 - GFF982 0.17 +1.7
300,907 - GFF982 0.17 +0.7
300,907 - GFF982 0.17 +0.4
300,907 - GFF982 0.17 +1.0
300,907 - GFF982 0.17 -0.1
300,907 - GFF982 0.17 +2.1
300,907 - GFF982 0.17 +0.9
300,907 - GFF982 0.17 -0.3
300,907 - GFF982 0.17 +0.4
300,907 - GFF982 0.17 +0.6
300,907 - GFF982 0.17 +0.8
301,086 + GFF982 0.34 +2.1
301,086 + GFF982 0.34 +0.8
301,086 + GFF982 0.34 +0.4
301,087 - GFF982 0.34 +1.4
301,087 - GFF982 0.34 +2.4
301,087 - GFF982 0.34 -0.2
301,222 - GFF982 0.46 +0.2
301,347 + GFF982 0.57 -0.1
301,347 + GFF982 0.57 +0.1
301,347 + GFF982 0.57 +0.1
301,347 + GFF982 0.57 +0.8
301,347 + GFF982 0.57 +1.1
301,347 + GFF982 0.57 +0.1
301,347 + GFF982 0.57 -1.0
301,347 + GFF982 0.57 -0.3
301,347 + GFF982 0.57 +0.1
301,347 + GFF982 0.57 +1.7
301,347 + GFF982 0.57 +0.5
301,347 + GFF982 0.57 +0.1
301,347 + GFF982 0.57 +0.4
301,347 + GFF982 0.57 -0.1
301,347 + GFF982 0.57 +0.5
301,347 + GFF982 0.57 +0.4
301,347 + GFF982 0.57 +0.0
301,347 + GFF982 0.57 +0.8
301,347 + GFF982 0.57 +1.5
301,347 + GFF982 0.57 +1.2
301,348 - GFF982 0.57 -0.2
301,348 - GFF982 0.57 +2.4
301,348 - GFF982 0.57 +0.2
301,348 - GFF982 0.57 +1.4
301,348 - GFF982 0.57 +1.8
301,348 - GFF982 0.57 +1.4
301,348 - GFF982 0.57 +1.7
301,348 - GFF982 0.57 +1.8
301,348 - GFF982 0.57 +1.4
301,348 - GFF982 0.57 +0.6
301,348 - GFF982 0.57 +0.6
301,348 - GFF982 0.57 +1.0
301,348 - GFF982 0.57 +0.1
301,348 - GFF982 0.57 +0.7
301,348 - GFF982 0.57 +0.6
301,348 - GFF982 0.57 +0.7
301,348 - GFF982 0.57 +0.6
301,348 - GFF982 0.57 +0.2
301,348 - GFF982 0.57 +1.8
301,348 - GFF982 0.57 +1.1
301,348 - GFF982 0.57 +0.4
301,440 - GFF982 0.66 +2.0
301,440 - GFF982 0.66 +0.5
301,536 + GFF982 0.74 -0.6
301,537 - GFF982 0.74 +1.0
301,647 + GFF982 0.84 +1.6
301,648 - GFF982 0.85 +1.9
301,648 - GFF982 0.85 +0.4
301,648 - GFF982 0.85 +0.8
301,648 - GFF982 0.85 +1.1
301,701 + GFF982 0.89 +1.4
301,701 + GFF982 0.89 +0.9
301,701 + GFF982 0.89 -1.1
301,701 + GFF982 0.89 -0.5
301,701 + GFF982 0.89 +1.7
301,701 + GFF982 0.89 +0.8
301,701 - GFF982 0.89 +0.8
301,702 - GFF982 0.89 +1.1
301,702 - GFF982 0.89 -1.0
301,702 - GFF982 0.89 +0.4
301,702 - GFF982 0.89 -0.8
301,702 - GFF982 0.89 +0.4
301,702 - GFF982 0.89 +0.1
301,702 - GFF982 0.89 +0.5

Or see this region's nucleotide sequence