Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF979 and GFF980 are separated by 53 nucleotides GFF980 and GFF981 are separated by 18 nucleotides
GFF979 - 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2), at 295,062 to 297,938
GFF979
GFF980 - Dihydrolipoamide succinyltransferase component (E2) of 2-oxoglutarate dehydrogenase complex (EC 2.3.1.61), at 297,992 to 299,263
GFF980
GFF981 - Dihydrolipoamide dehydrogenase of 2-oxoglutarate dehydrogenase (EC 1.8.1.4), at 299,282 to 300,712
GFF981
Position (kb) 297
298
299
300 Strain fitness (log2 ratio) -1
0
1 at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.936 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand at 297.939 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 297,936 + -0.2 297,936 + -0.0 297,936 + -1.6 297,936 + -0.6 297,936 + -0.3 297,936 + -0.1 297,936 + +0.0 297,936 + -0.1 297,936 + -1.5 297,936 + -0.6 297,936 + +0.1 297,936 + -1.6 297,936 + -1.0 297,936 + -0.5 297,936 + +0.4 297,936 + +0.3 297,936 + -0.6 297,936 + -0.9 297,936 + -0.4 297,939 + -0.7 297,939 + -0.1 297,939 + -0.5 297,939 + -1.0 297,939 + -0.8 297,939 + +0.4 297,939 + +0.1 297,939 + +0.1 297,939 + +0.2 297,939 + +0.6 297,939 + +0.9 297,939 + -0.6
Or see this region's nucleotide sequence