Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF97 and GFF98 are separated by 412 nucleotides GFF98 and GFF99 overlap by 1 nucleotides GFF99 and GFF100 overlap by 4 nucleotides
GFF97 - Filamentous haemagglutinin family outer membrane protein associated with VreARI signalling system, at 110,545 to 123,438
GFF97
GFF98 - Sigma factor regulator VreR (cytoplasmic membrane-localized) of trans-envelope signaling system, at 123,851 to 124,849
GFF98
GFF99 - KEGG: RNA polymerase sigma-70 factor, ECF subfamily, at 124,849 to 125,409
GFF99
GFF100 - no description, at 125,406 to 126,104
GFF100
Position (kb) 123
124
125 Strain fitness (log2 ratio) -2
-1
0
1 at 123.803 kb on + strand at 123.804 kb on - strand at 123.804 kb on - strand at 123.804 kb on - strand at 124.853 kb on + strand at 124.854 kb on - strand at 124.854 kb on - strand at 125.194 kb on + strand, within GFF99 at 125.194 kb on + strand, within GFF99 at 125.194 kb on + strand, within GFF99 at 125.360 kb on + strand at 125.360 kb on + strand at 125.547 kb on + strand, within GFF100 at 125.548 kb on - strand, within GFF100 at 125.548 kb on - strand, within GFF100 at 125.548 kb on - strand, within GFF100 at 125.548 kb on - strand, within GFF100 at 125.651 kb on - strand, within GFF100
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 123,803 + +0.5 123,804 - +0.3 123,804 - +0.1 123,804 - -2.4 124,853 + -1.3 124,854 - +1.5 124,854 - -1.4 125,194 + GFF99 0.61 +0.1 125,194 + GFF99 0.61 +0.7 125,194 + GFF99 0.61 -0.3 125,360 + +0.9 125,360 + +0.0 125,547 + GFF100 0.20 -0.8 125,548 - GFF100 0.20 -0.4 125,548 - GFF100 0.20 +0.8 125,548 - GFF100 0.20 +0.5 125,548 - GFF100 0.20 +0.9 125,651 - GFF100 0.35 -1.6
Or see this region's nucleotide sequence