Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF963 and GFF964 are separated by 5 nucleotides GFF964 and GFF965 are separated by 76 nucleotides GFF965 and GFF966 overlap by 4 nucleotides
GFF963 - Oligopeptidase A (EC 3.4.24.70), at 273,738 to 275,807
GFF963
GFF964 - Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) / Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5), at 275,813 to 276,664
GFF964
GFF965 - Two-component transcriptional response regulator, LuxR family, at 276,741 to 277,373
GFF965
GFF966 - Probable two-component transmembrane sensor histidine kinase transcription regulator protein (EC 2.7.3.-), at 277,370 to 279,910
GFF966
Position (kb)
275
276
277 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.813 kb on + strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.814 kb on - strand, within GFF963 at 274.897 kb on + strand, within GFF963 at 274.996 kb on + strand, within GFF963 at 274.996 kb on + strand, within GFF963 at 274.996 kb on + strand, within GFF963 at 274.997 kb on - strand, within GFF963 at 275.098 kb on + strand, within GFF963 at 275.098 kb on + strand, within GFF963 at 275.099 kb on - strand, within GFF963 at 275.099 kb on - strand, within GFF963 at 275.099 kb on - strand, within GFF963 at 275.099 kb on - strand, within GFF963 at 275.099 kb on - strand, within GFF963 at 275.107 kb on + strand, within GFF963 at 275.107 kb on + strand, within GFF963 at 275.108 kb on - strand, within GFF963 at 275.108 kb on - strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.158 kb on + strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.159 kb on - strand, within GFF963 at 275.185 kb on + strand, within GFF963 at 275.185 kb on + strand, within GFF963 at 275.185 kb on + strand, within GFF963 at 275.185 kb on + strand, within GFF963 at 275.185 kb on + strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.186 kb on - strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.272 kb on + strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.273 kb on - strand, within GFF963 at 275.276 kb on - strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.470 kb on + strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.471 kb on - strand, within GFF963 at 275.480 kb on - strand, within GFF963 at 275.480 kb on - strand, within GFF963 at 275.543 kb on - strand, within GFF963 at 275.543 kb on - strand, within GFF963 at 275.543 kb on - strand, within GFF963 at 275.717 kb on + strand at 275.718 kb on - strand at 275.814 kb on + strand at 275.814 kb on + strand at 275.814 kb on + strand at 275.814 kb on + strand at 275.814 kb on + strand at 275.814 kb on + strand at 275.814 kb on + strand at 275.814 kb on + strand at 275.815 kb on - strand at 275.815 kb on - strand at 275.815 kb on - strand at 275.815 kb on - strand at 275.815 kb on - strand at 275.815 kb on - strand at 276.683 kb on + strand at 276.735 kb on + strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.736 kb on - strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.742 kb on + strand at 276.743 kb on - strand at 276.743 kb on - strand at 276.743 kb on - strand at 277.235 kb on - strand, within GFF965 at 277.280 kb on - strand, within GFF965 at 277.343 kb on - strand at 277.343 kb on - strand at 277.380 kb on - strand at 277.380 kb on - strand at 277.380 kb on - strand at 277.397 kb on - strand at 277.397 kb on - strand at 277.397 kb on - strand at 277.397 kb on - strand at 277.400 kb on + strand at 277.471 kb on - strand at 277.471 kb on - strand at 277.471 kb on - strand at 277.576 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 274,813 + GFF963 0.52 +0.4 274,813 + GFF963 0.52 -0.0 274,813 + GFF963 0.52 +0.1 274,813 + GFF963 0.52 -0.1 274,813 + GFF963 0.52 -1.0 274,813 + GFF963 0.52 -0.4 274,813 + GFF963 0.52 -2.3 274,813 + GFF963 0.52 -0.3 274,813 + GFF963 0.52 -0.2 274,813 + GFF963 0.52 +0.2 274,813 + GFF963 0.52 -0.9 274,813 + GFF963 0.52 +0.9 274,813 + GFF963 0.52 -0.8 274,813 + GFF963 0.52 -0.1 274,813 + GFF963 0.52 +1.1 274,813 + GFF963 0.52 -0.3 274,813 + GFF963 0.52 +0.4 274,813 + GFF963 0.52 +1.4 274,813 + GFF963 0.52 -0.1 274,813 + GFF963 0.52 -0.2 274,814 - GFF963 0.52 -0.5 274,814 - GFF963 0.52 -2.2 274,814 - GFF963 0.52 +0.7 274,814 - GFF963 0.52 -1.4 274,814 - GFF963 0.52 +0.2 274,814 - GFF963 0.52 -0.1 274,814 - GFF963 0.52 +0.6 274,814 - GFF963 0.52 -0.2 274,814 - GFF963 0.52 -1.5 274,814 - GFF963 0.52 +0.8 274,814 - GFF963 0.52 -0.9 274,814 - GFF963 0.52 -1.2 274,814 - GFF963 0.52 +0.3 274,814 - GFF963 0.52 -0.6 274,814 - GFF963 0.52 -1.4 274,814 - GFF963 0.52 +0.2 274,814 - GFF963 0.52 -0.1 274,814 - GFF963 0.52 -0.9 274,897 + GFF963 0.56 -0.0 274,996 + GFF963 0.61 +0.0 274,996 + GFF963 0.61 +0.1 274,996 + GFF963 0.61 -0.4 274,997 - GFF963 0.61 +0.3 275,098 + GFF963 0.66 +0.0 275,098 + GFF963 0.66 -0.2 275,099 - GFF963 0.66 -0.5 275,099 - GFF963 0.66 +0.1 275,099 - GFF963 0.66 -0.1 275,099 - GFF963 0.66 -1.2 275,099 - GFF963 0.66 +0.5 275,107 + GFF963 0.66 +0.7 275,107 + GFF963 0.66 -0.4 275,108 - GFF963 0.66 -0.2 275,108 - GFF963 0.66 -0.5 275,158 + GFF963 0.69 +0.7 275,158 + GFF963 0.69 -0.4 275,158 + GFF963 0.69 -0.2 275,158 + GFF963 0.69 +0.2 275,158 + GFF963 0.69 -0.3 275,158 + GFF963 0.69 -0.6 275,158 + GFF963 0.69 +0.2 275,158 + GFF963 0.69 -0.7 275,159 - GFF963 0.69 -0.2 275,159 - GFF963 0.69 +0.2 275,159 - GFF963 0.69 -0.2 275,159 - GFF963 0.69 +1.2 275,159 - GFF963 0.69 +0.1 275,159 - GFF963 0.69 +0.2 275,159 - GFF963 0.69 -0.0 275,159 - GFF963 0.69 -0.6 275,185 + GFF963 0.70 -0.6 275,185 + GFF963 0.70 -0.4 275,185 + GFF963 0.70 -0.0 275,185 + GFF963 0.70 +0.3 275,185 + GFF963 0.70 -0.8 275,186 - GFF963 0.70 -0.4 275,186 - GFF963 0.70 +0.5 275,186 - GFF963 0.70 +1.8 275,186 - GFF963 0.70 -0.6 275,186 - GFF963 0.70 -0.8 275,186 - GFF963 0.70 -1.0 275,186 - GFF963 0.70 +0.9 275,186 - GFF963 0.70 -1.2 275,272 + GFF963 0.74 -1.0 275,272 + GFF963 0.74 +1.7 275,272 + GFF963 0.74 -0.2 275,272 + GFF963 0.74 +0.7 275,272 + GFF963 0.74 +0.3 275,272 + GFF963 0.74 -0.6 275,272 + GFF963 0.74 -0.3 275,272 + GFF963 0.74 +0.3 275,273 - GFF963 0.74 +0.0 275,273 - GFF963 0.74 +1.0 275,273 - GFF963 0.74 -1.8 275,273 - GFF963 0.74 +0.3 275,273 - GFF963 0.74 -0.4 275,273 - GFF963 0.74 -0.4 275,273 - GFF963 0.74 -1.4 275,273 - GFF963 0.74 +0.2 275,276 - GFF963 0.74 +0.6 275,470 + GFF963 0.84 +0.0 275,470 + GFF963 0.84 +0.5 275,470 + GFF963 0.84 +0.1 275,470 + GFF963 0.84 +0.2 275,470 + GFF963 0.84 +0.4 275,470 + GFF963 0.84 -1.4 275,470 + GFF963 0.84 -0.8 275,470 + GFF963 0.84 -2.2 275,470 + GFF963 0.84 -0.2 275,470 + GFF963 0.84 +0.1 275,470 + GFF963 0.84 -0.3 275,471 - GFF963 0.84 -1.4 275,471 - GFF963 0.84 -0.1 275,471 - GFF963 0.84 -0.7 275,471 - GFF963 0.84 +0.0 275,471 - GFF963 0.84 +0.4 275,471 - GFF963 0.84 +2.7 275,471 - GFF963 0.84 -0.6 275,471 - GFF963 0.84 +0.2 275,471 - GFF963 0.84 -0.2 275,471 - GFF963 0.84 +0.1 275,480 - GFF963 0.84 -0.6 275,480 - GFF963 0.84 -0.1 275,543 - GFF963 0.87 -0.6 275,543 - GFF963 0.87 +0.5 275,543 - GFF963 0.87 +1.0 275,717 + -0.9 275,718 - +0.2 275,814 + -0.0 275,814 + -0.4 275,814 + -1.0 275,814 + -0.1 275,814 + -0.9 275,814 + -0.7 275,814 + -0.1 275,814 + -0.4 275,815 - -0.1 275,815 - -1.0 275,815 - -0.1 275,815 - -0.2 275,815 - -0.8 275,815 - -0.9 276,683 + +1.9 276,735 + -1.0 276,736 - +0.2 276,736 - +0.1 276,736 - +0.7 276,736 - -0.6 276,736 - -0.1 276,736 - -0.4 276,736 - -0.2 276,742 + +0.3 276,742 + -0.1 276,742 + +0.9 276,742 + +0.5 276,742 + +0.5 276,742 + +0.8 276,742 + -0.8 276,742 + +1.0 276,743 - -0.1 276,743 - +0.2 276,743 - -0.3 277,235 - GFF965 0.78 -1.2 277,280 - GFF965 0.85 +1.4 277,343 - -2.3 277,343 - +0.4 277,380 - +3.7 277,380 - -1.2 277,380 - -1.2 277,397 - -1.4 277,397 - -1.0 277,397 - +0.1 277,397 - -0.6 277,400 + +0.4 277,471 - -0.6 277,471 - -1.6 277,471 - -1.4 277,576 - -2.2
Or see this region's nucleotide sequence