Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF958 and GFF959 are separated by 51 nucleotides GFF959 and GFF960 are separated by 20 nucleotides GFF960 and GFF961 are separated by 40 nucleotides GFF961 and GFF962 are separated by 18 nucleotides
GFF958 - ElaB protein, at 270,114 to 270,410
GFF958
GFF959 - Phage holin, at 270,462 to 271,187
GFF959
GFF960 - Dihydroorotate dehydrogenase (quinone) (EC 1.3.5.2), at 271,208 to 272,287
GFF960
GFF961 - Ribose-5-phosphate isomerase A (EC 5.3.1.6), at 272,328 to 273,041
GFF961
GFF962 - FIG00932012: hypothetical protein, at 273,060 to 273,707
GFF962
Position (kb) 271
272
273 Strain fitness (log2 ratio) -2
-1
0
1
2 at 270.327 kb on + strand, within GFF958 at 270.327 kb on + strand, within GFF958 at 270.327 kb on + strand, within GFF958 at 270.327 kb on + strand, within GFF958 at 270.328 kb on - strand, within GFF958 at 270.328 kb on - strand, within GFF958 at 270.328 kb on - strand, within GFF958 at 270.328 kb on - strand, within GFF958 at 270.328 kb on - strand, within GFF958 at 270.696 kb on + strand, within GFF959 at 270.697 kb on - strand, within GFF959 at 271.287 kb on + strand at 271.287 kb on + strand at 271.287 kb on + strand at 271.287 kb on + strand at 271.288 kb on - strand at 271.776 kb on + strand, within GFF960 at 271.776 kb on + strand, within GFF960 at 271.776 kb on + strand, within GFF960 at 271.777 kb on - strand, within GFF960 at 272.154 kb on + strand, within GFF960 at 272.155 kb on - strand, within GFF960 at 272.292 kb on + strand at 272.292 kb on + strand at 272.293 kb on - strand at 272.293 kb on - strand at 273.152 kb on + strand, within GFF962 at 273.152 kb on + strand, within GFF962 at 273.256 kb on + strand, within GFF962 at 273.256 kb on + strand, within GFF962 at 273.256 kb on + strand, within GFF962 at 273.256 kb on + strand, within GFF962 at 273.256 kb on + strand, within GFF962 at 273.256 kb on + strand, within GFF962 at 273.257 kb on - strand, within GFF962
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 270,327 + GFF958 0.72 +0.4 270,327 + GFF958 0.72 +0.8 270,327 + GFF958 0.72 +0.4 270,327 + GFF958 0.72 -0.9 270,328 - GFF958 0.72 +0.4 270,328 - GFF958 0.72 +0.9 270,328 - GFF958 0.72 -0.2 270,328 - GFF958 0.72 +0.8 270,328 - GFF958 0.72 -2.2 270,696 + GFF959 0.32 -0.7 270,697 - GFF959 0.32 +0.4 271,287 + -0.6 271,287 + -1.2 271,287 + -2.2 271,287 + -0.6 271,288 - -0.2 271,776 + GFF960 0.53 -0.6 271,776 + GFF960 0.53 -2.2 271,776 + GFF960 0.53 -0.2 271,777 - GFF960 0.53 -0.6 272,154 + GFF960 0.88 -0.6 272,155 - GFF960 0.88 -0.4 272,292 + -1.6 272,292 + +0.4 272,293 - -0.6 272,293 - +1.0 273,152 + GFF962 0.14 -0.4 273,152 + GFF962 0.14 +0.1 273,256 + GFF962 0.30 +0.5 273,256 + GFF962 0.30 -0.2 273,256 + GFF962 0.30 +2.4 273,256 + GFF962 0.30 +0.0 273,256 + GFF962 0.30 +0.1 273,256 + GFF962 0.30 -0.2 273,257 - GFF962 0.30 -0.2
Or see this region's nucleotide sequence