Strain Fitness in Variovorax sp. SCN45 around GFF952

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF951 and GFF952 are separated by 6 nucleotidesGFF952 and GFF953 overlap by 1 nucleotidesGFF953 and GFF954 are separated by 193 nucleotides GFF951 - Chaperone protein HscA, at 264,369 to 266,234 GFF951 GFF952 - Ferredoxin, 2Fe-2S, at 266,241 to 266,579 GFF952 GFF953 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 266,579 to 267,301 GFF953 GFF954 - hypothetical protein, at 267,495 to 267,839 GFF954 Position (kb) 266 267Strain fitness (log2 ratio) -2 -1 0 1 2at 266.232 kb on + strandat 266.232 kb on + strandat 266.232 kb on + strandat 267.244 kb on + strandat 267.244 kb on + strandat 267.244 kb on + strandat 267.328 kb on + strandat 267.497 kb on - strandat 267.497 kb on - strandat 267.497 kb on - strandat 267.527 kb on + strandat 267.528 kb on - strandat 267.528 kb on - strandat 267.541 kb on + strand, within GFF954at 267.541 kb on + strand, within GFF954at 267.542 kb on - strand, within GFF954at 267.542 kb on - strand, within GFF954at 267.542 kb on - strand, within GFF954at 267.542 kb on - strand, within GFF954at 267.542 kb on - strand, within GFF954

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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266,232 + +1.0
266,232 + -1.4
266,232 + -0.7
267,244 + +0.0
267,244 + +0.1
267,244 + -0.2
267,328 + -0.0
267,497 - -0.6
267,497 - +0.5
267,497 - +2.0
267,527 + +0.0
267,528 - +0.4
267,528 - -0.2
267,541 + GFF954 0.13 -0.7
267,541 + GFF954 0.13 -2.5
267,542 - GFF954 0.14 +1.6
267,542 - GFF954 0.14 -0.3
267,542 - GFF954 0.14 -0.5
267,542 - GFF954 0.14 +0.3
267,542 - GFF954 0.14 +0.2

Or see this region's nucleotide sequence