Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF948 and GFF949 are separated by 1 nucleotides GFF949 and GFF950 are separated by 63 nucleotides GFF950 and GFF951 are separated by 91 nucleotides GFF951 and GFF952 are separated by 6 nucleotides GFF952 and GFF953 overlap by 1 nucleotides
GFF948 - Iron-sulfur cluster assembly scaffold protein IscU, at 262,957 to 263,370
GFF948
GFF949 - Iron-sulfur cluster assembly iron binding protein IscA, at 263,372 to 263,695
GFF949
GFF950 - Chaperone protein HscB, at 263,759 to 264,277
GFF950
GFF951 - Chaperone protein HscA, at 264,369 to 266,234
GFF951
GFF952 - Ferredoxin, 2Fe-2S, at 266,241 to 266,579
GFF952
GFF953 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 266,579 to 267,301
GFF953
Position (kb) 264
265
266
267 Strain fitness (log2 ratio) -2
-1
0
1 at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.275 kb on + strand at 264.276 kb on - strand at 264.276 kb on - strand at 264.276 kb on - strand at 264.276 kb on - strand at 264.276 kb on - strand at 264.276 kb on - strand at 264.310 kb on + strand at 264.310 kb on + strand at 264.310 kb on + strand at 264.310 kb on + strand at 266.232 kb on + strand at 266.232 kb on + strand at 266.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 264,275 + +1.4 264,275 + +0.2 264,275 + +0.6 264,275 + +0.1 264,275 + -0.8 264,275 + -1.0 264,275 + +0.1 264,275 + +0.7 264,275 + -0.1 264,275 + -0.2 264,275 + -0.2 264,275 + +0.4 264,275 + -0.7 264,275 + +0.1 264,275 + -0.4 264,275 + -0.3 264,275 + -0.2 264,275 + +1.0 264,275 + +1.4 264,275 + -0.6 264,275 + +0.0 264,275 + -0.3 264,275 + +1.0 264,275 + +0.2 264,275 + -0.1 264,275 + -0.4 264,275 + -2.0 264,275 + +0.3 264,275 + -0.2 264,276 - +1.1 264,276 - +0.1 264,276 - -0.1 264,276 - -0.4 264,276 - -0.2 264,276 - -0.4 264,310 + -1.8 264,310 + -1.8 264,310 + +0.6 264,310 + -2.1 266,232 + +1.0 266,232 + -1.4 266,232 + -0.7
Or see this region's nucleotide sequence