Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF945 and GFF946 are separated by 242 nucleotides GFF946 and GFF947 are separated by 100 nucleotides GFF947 and GFF948 are separated by 50 nucleotides GFF948 and GFF949 are separated by 1 nucleotides GFF949 and GFF950 are separated by 63 nucleotides
GFF945 - Excinuclease ABC subunit B, at 258,698 to 260,806
GFF945
GFF946 - Iron-sulfur cluster regulator IscR, at 261,049 to 261,585
GFF946
GFF947 - Cysteine desulfurase (EC 2.8.1.7) => IscS, at 261,686 to 262,906
GFF947
GFF948 - Iron-sulfur cluster assembly scaffold protein IscU, at 262,957 to 263,370
GFF948
GFF949 - Iron-sulfur cluster assembly iron binding protein IscA, at 263,372 to 263,695
GFF949
GFF950 - Chaperone protein HscB, at 263,759 to 264,277
GFF950
Position (kb) 261
262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 260.804 kb on + strand at 260.804 kb on + strand at 260.804 kb on + strand at 260.804 kb on + strand at 260.804 kb on + strand at 260.805 kb on - strand at 260.805 kb on - strand at 260.805 kb on - strand at 260.805 kb on - strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.813 kb on + strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.814 kb on - strand at 260.857 kb on - strand at 260.884 kb on + strand at 260.885 kb on - strand at 260.885 kb on - strand at 260.885 kb on - strand at 260.886 kb on + strand at 260.886 kb on + strand at 260.944 kb on - strand at 260.944 kb on - strand at 261.027 kb on + strand at 261.027 kb on + strand at 261.169 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.209 kb on + strand, within GFF946 at 261.241 kb on + strand, within GFF946 at 261.241 kb on + strand, within GFF946 at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand at 262.951 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 260,804 + -0.9 260,804 + -0.5 260,804 + +0.1 260,804 + -1.9 260,804 + -1.4 260,805 - -0.2 260,805 - +0.5 260,805 - -0.6 260,805 - -1.2 260,813 + -0.6 260,813 + +1.4 260,813 + -0.6 260,813 + -0.3 260,813 + -0.9 260,813 + +1.0 260,814 - -0.5 260,814 - -0.0 260,814 - +0.0 260,814 - +0.5 260,814 - +0.3 260,814 - -0.5 260,814 - +0.1 260,857 - -1.6 260,884 + -0.6 260,885 - +0.4 260,885 - +0.0 260,885 - +0.8 260,886 + -1.4 260,886 + +0.4 260,944 - -0.1 260,944 - -0.5 261,027 + +0.0 261,027 + +1.5 261,169 + GFF946 0.22 -2.9 261,209 + GFF946 0.30 -1.4 261,209 + GFF946 0.30 -1.6 261,209 + GFF946 0.30 -2.2 261,209 + GFF946 0.30 -1.4 261,241 + GFF946 0.36 -1.2 261,241 + GFF946 0.36 -0.2 262,951 + +0.3 262,951 + -3.7 262,951 + +0.3 262,951 + -1.0 262,951 + +1.0 262,951 + +0.8 262,951 + +0.5 262,951 + +0.2 262,951 + -1.0 262,951 + +1.0 262,951 + -0.1 262,951 + -0.1 262,951 + +1.4 262,951 + +0.0 262,951 + -1.0 262,951 + -0.0 262,951 + -1.5 262,951 + +0.4
Or see this region's nucleotide sequence