Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF91 and GFF92 are separated by 0 nucleotides GFF92 and GFF93 are separated by 2 nucleotides GFF93 and GFF94 are separated by 99 nucleotides GFF94 and GFF95 are separated by 27 nucleotides GFF95 and GFF96 are separated by 58 nucleotides
GFF91 - General secretion pathway protein J, at 107,002 to 107,619
GFF91
GFF92 - KEGG: general secretion pathway protein I, at 107,620 to 107,997
GFF92
GFF93 - General secretion pathway protein H, at 108,000 to 108,494
GFF93
GFF94 - hypothetical protein, at 108,594 to 109,037
GFF94
GFF95 - hypothetical protein, at 109,065 to 109,703
GFF95
GFF96 - RNA polymerase ECF-type sigma factor, at 109,762 to 110,424
GFF96
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-2
-1
0
1 at 107.621 kb on + strand at 107.622 kb on - strand at 107.684 kb on + strand, within GFF92 at 107.684 kb on + strand, within GFF92 at 107.684 kb on + strand, within GFF92 at 107.684 kb on + strand, within GFF92 at 107.685 kb on - strand, within GFF92 at 107.685 kb on - strand, within GFF92 at 108.013 kb on + strand at 108.014 kb on - strand at 108.014 kb on - strand at 108.497 kb on + strand at 108.498 kb on - strand at 108.498 kb on - strand at 108.498 kb on - strand at 108.498 kb on - strand at 108.558 kb on - strand at 108.633 kb on + strand at 108.633 kb on + strand at 108.633 kb on + strand at 108.634 kb on - strand at 109.067 kb on - strand at 109.224 kb on + strand, within GFF95 at 109.336 kb on + strand, within GFF95 at 109.336 kb on + strand, within GFF95 at 109.336 kb on + strand, within GFF95 at 109.336 kb on + strand, within GFF95 at 109.337 kb on - strand, within GFF95 at 109.378 kb on + strand, within GFF95 at 109.378 kb on + strand, within GFF95 at 109.378 kb on + strand, within GFF95 at 109.378 kb on + strand, within GFF95 at 109.378 kb on + strand, within GFF95 at 109.378 kb on + strand, within GFF95
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 107,621 + -0.2 107,622 - +0.7 107,684 + GFF92 0.17 -1.8 107,684 + GFF92 0.17 +0.3 107,684 + GFF92 0.17 +0.2 107,684 + GFF92 0.17 -0.2 107,685 - GFF92 0.17 +0.6 107,685 - GFF92 0.17 -0.6 108,013 + -0.4 108,014 - -1.3 108,014 - -0.5 108,497 + -0.2 108,498 - -0.6 108,498 - +1.1 108,498 - +0.5 108,498 - +1.0 108,558 - +0.9 108,633 + -0.4 108,633 + -0.6 108,633 + -0.6 108,634 - +0.3 109,067 - -0.5 109,224 + GFF95 0.25 -0.8 109,336 + GFF95 0.42 -0.0 109,336 + GFF95 0.42 +0.4 109,336 + GFF95 0.42 +0.0 109,336 + GFF95 0.42 -0.4 109,337 - GFF95 0.43 -0.1 109,378 + GFF95 0.49 -1.0 109,378 + GFF95 0.49 -1.1 109,378 + GFF95 0.49 +0.0 109,378 + GFF95 0.49 -0.4 109,378 + GFF95 0.49 -0.1 109,378 + GFF95 0.49 -0.6
Or see this region's nucleotide sequence