Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF903 and GFF904 are separated by 13 nucleotides GFF904 and GFF905 are separated by 8 nucleotides GFF905 and GFF906 are separated by 11 nucleotides
GFF903 - Acetolactate synthase large subunit (EC 2.2.1.6), at 213,935 to 215,713
GFF903
GFF904 - hypothetical protein, at 215,727 to 216,158
GFF904
GFF905 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 216,167 to 216,943
GFF905
GFF906 - Transcriptional regulator, at 216,955 to 217,422
GFF906
Position (kb) 215
216
217 Strain fitness (log2 ratio) -2
-1
0
1
2 at 215.202 kb on - strand, within GFF903 at 215.261 kb on + strand, within GFF903 at 215.262 kb on - strand, within GFF903 at 215.417 kb on + strand, within GFF903 at 215.417 kb on + strand, within GFF903 at 215.417 kb on + strand, within GFF903 at 215.418 kb on - strand, within GFF903 at 215.473 kb on + strand, within GFF903 at 215.473 kb on + strand, within GFF903 at 215.474 kb on - strand, within GFF903 at 215.474 kb on - strand, within GFF903 at 215.699 kb on + strand at 215.700 kb on - strand at 215.700 kb on - strand at 215.923 kb on + strand, within GFF904 at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.237 kb on + strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.238 kb on - strand at 216.430 kb on - strand, within GFF905 at 216.551 kb on + strand, within GFF905 at 216.551 kb on + strand, within GFF905 at 216.552 kb on - strand, within GFF905 at 216.568 kb on - strand, within GFF905 at 216.587 kb on + strand, within GFF905 at 216.587 kb on + strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.588 kb on - strand, within GFF905 at 216.618 kb on + strand, within GFF905 at 216.690 kb on + strand, within GFF905 at 216.690 kb on + strand, within GFF905 at 216.782 kb on + strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.783 kb on - strand, within GFF905 at 216.811 kb on - strand, within GFF905 at 216.811 kb on - strand, within GFF905 at 216.965 kb on + strand at 216.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 215,202 - GFF903 0.71 -0.6 215,261 + GFF903 0.75 -0.3 215,262 - GFF903 0.75 +1.7 215,417 + GFF903 0.83 +0.2 215,417 + GFF903 0.83 -0.4 215,417 + GFF903 0.83 -1.2 215,418 - GFF903 0.83 +0.2 215,473 + GFF903 0.86 +1.1 215,473 + GFF903 0.86 -0.5 215,474 - GFF903 0.87 -1.5 215,474 - GFF903 0.87 -0.4 215,699 + -1.8 215,700 - +2.0 215,700 - -0.5 215,923 + GFF904 0.45 -0.4 216,237 + +0.4 216,237 + +0.0 216,237 + +0.2 216,237 + -1.4 216,237 + -0.1 216,237 + +0.4 216,237 + -1.6 216,237 + +0.8 216,238 - -0.1 216,238 - -0.0 216,238 - +0.4 216,238 - +0.6 216,238 - -0.8 216,430 - GFF905 0.34 +0.4 216,551 + GFF905 0.49 -0.8 216,551 + GFF905 0.49 -0.6 216,552 - GFF905 0.50 -0.1 216,568 - GFF905 0.52 -0.6 216,587 + GFF905 0.54 -0.9 216,587 + GFF905 0.54 +0.2 216,588 - GFF905 0.54 -2.2 216,588 - GFF905 0.54 -0.2 216,588 - GFF905 0.54 -0.2 216,618 + GFF905 0.58 +0.1 216,690 + GFF905 0.67 -1.5 216,690 + GFF905 0.67 -0.7 216,782 + GFF905 0.79 -0.0 216,783 - GFF905 0.79 -0.6 216,783 - GFF905 0.79 -0.1 216,783 - GFF905 0.79 -0.4 216,783 - GFF905 0.79 +0.8 216,811 - GFF905 0.83 -0.1 216,811 - GFF905 0.83 -2.1 216,965 + -1.4 216,966 - -1.8
Or see this region's nucleotide sequence