Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt
GFF7446 - Transposase, at 60 to 1,385
GFF7446
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-1
0
1 at 0.048 kb on + strand at 0.048 kb on + strand at 0.048 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.072 kb on + strand at 0.081 kb on + strand at 0.081 kb on + strand at 0.081 kb on + strand at 0.081 kb on + strand at 0.082 kb on - strand at 0.082 kb on - strand at 0.082 kb on - strand at 0.082 kb on - strand at 0.150 kb on + strand at 0.150 kb on + strand at 0.150 kb on + strand at 0.150 kb on + strand at 0.150 kb on + strand at 0.150 kb on + strand at 0.150 kb on + strand at 0.150 kb on + strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.151 kb on - strand at 0.205 kb on - strand, within GFF7446 at 0.205 kb on - strand, within GFF7446 at 0.205 kb on - strand, within GFF7446 at 0.205 kb on - strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.267 kb on + strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.268 kb on - strand, within GFF7446 at 0.273 kb on + strand, within GFF7446 at 0.273 kb on + strand, within GFF7446 at 0.274 kb on - strand, within GFF7446 at 0.357 kb on + strand, within GFF7446 at 0.357 kb on + strand, within GFF7446 at 0.357 kb on + strand, within GFF7446 at 0.357 kb on + strand, within GFF7446 at 0.357 kb on + strand, within GFF7446 at 0.358 kb on - strand, within GFF7446 at 0.358 kb on - strand, within GFF7446 at 0.358 kb on - strand, within GFF7446 at 0.358 kb on - strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.531 kb on + strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.532 kb on - strand, within GFF7446 at 0.801 kb on + strand, within GFF7446 at 0.801 kb on + strand, within GFF7446 at 0.801 kb on + strand, within GFF7446 at 0.802 kb on - strand, within GFF7446 at 0.802 kb on - strand, within GFF7446 at 0.802 kb on - strand, within GFF7446 at 0.802 kb on - strand, within GFF7446 at 0.802 kb on - strand, within GFF7446 at 0.802 kb on - strand, within GFF7446 at 0.891 kb on + strand, within GFF7446 at 0.891 kb on + strand, within GFF7446 at 0.891 kb on + strand, within GFF7446 at 0.892 kb on - strand, within GFF7446 at 0.897 kb on + strand, within GFF7446 at 0.897 kb on + strand, within GFF7446 at 0.897 kb on + strand, within GFF7446 at 0.897 kb on + strand, within GFF7446 at 0.897 kb on + strand, within GFF7446 at 0.897 kb on + strand, within GFF7446 at 0.897 kb on + strand, within GFF7446 at 0.898 kb on - strand, within GFF7446 at 0.898 kb on - strand, within GFF7446 at 0.898 kb on - strand, within GFF7446 at 0.898 kb on - strand, within GFF7446 at 0.898 kb on - strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.903 kb on + strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 0.904 kb on - strand, within GFF7446 at 1.164 kb on + strand, within GFF7446 at 1.164 kb on + strand, within GFF7446 at 1.164 kb on + strand, within GFF7446 at 1.164 kb on + strand, within GFF7446 at 1.164 kb on + strand, within GFF7446 at 1.164 kb on + strand, within GFF7446 at 1.165 kb on - strand, within GFF7446 at 1.221 kb on + strand, within GFF7446 at 1.222 kb on - strand, within GFF7446 at 1.437 kb on + strand at 1.437 kb on + strand at 1.437 kb on + strand at 1.437 kb on + strand at 1.437 kb on + strand at 1.437 kb on + strand at 1.438 kb on - strand at 1.438 kb on - strand at 1.438 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 48 + -0.0 48 + -0.9 48 + +0.3 72 + -0.1 72 + +1.0 72 + -1.0 72 + -1.0 72 + +0.6 72 + +0.2 72 + -0.6 72 + +0.3 72 + -1.0 72 + -0.4 72 + -0.7 72 + -0.2 81 + +0.3 81 + +0.1 81 + -0.0 81 + +0.5 82 - -0.6 82 - +0.1 82 - +0.2 82 - -0.8 150 + +0.0 150 + -0.8 150 + +0.4 150 + +0.3 150 + -0.9 150 + +0.0 150 + -1.2 150 + -0.7 151 - +0.6 151 - +1.6 151 - +0.8 151 - -0.9 151 - +0.6 151 - +1.4 151 - +0.2 151 - -0.5 151 - -0.5 151 - +0.6 151 - +0.9 151 - -0.6 151 - -0.1 151 - +1.0 151 - -0.0 151 - -0.5 151 - +0.7 205 - GFF7446 0.11 +0.1 205 - GFF7446 0.11 -0.1 205 - GFF7446 0.11 +0.3 205 - GFF7446 0.11 -0.5 267 + GFF7446 0.16 +0.5 267 + GFF7446 0.16 +0.5 267 + GFF7446 0.16 +0.0 267 + GFF7446 0.16 -0.3 267 + GFF7446 0.16 -0.2 267 + GFF7446 0.16 -1.0 267 + GFF7446 0.16 -0.4 267 + GFF7446 0.16 -0.4 267 + GFF7446 0.16 -0.8 267 + GFF7446 0.16 -1.3 267 + GFF7446 0.16 +0.5 267 + GFF7446 0.16 -0.5 267 + GFF7446 0.16 -0.1 268 - GFF7446 0.16 -1.0 268 - GFF7446 0.16 +0.5 268 - GFF7446 0.16 -1.0 268 - GFF7446 0.16 +0.5 268 - GFF7446 0.16 +0.4 268 - GFF7446 0.16 +0.2 268 - GFF7446 0.16 -0.7 268 - GFF7446 0.16 -0.3 268 - GFF7446 0.16 +0.3 268 - GFF7446 0.16 -1.1 273 + GFF7446 0.16 +0.3 273 + GFF7446 0.16 -0.0 274 - GFF7446 0.16 -0.1 357 + GFF7446 0.22 -0.1 357 + GFF7446 0.22 +0.7 357 + GFF7446 0.22 +0.2 357 + GFF7446 0.22 +0.4 357 + GFF7446 0.22 +0.1 358 - GFF7446 0.22 +0.0 358 - GFF7446 0.22 -0.2 358 - GFF7446 0.22 +1.2 358 - GFF7446 0.22 +1.6 531 + GFF7446 0.36 -0.0 531 + GFF7446 0.36 -0.2 531 + GFF7446 0.36 -1.1 531 + GFF7446 0.36 +0.3 531 + GFF7446 0.36 -0.3 531 + GFF7446 0.36 -1.1 531 + GFF7446 0.36 -1.0 531 + GFF7446 0.36 -1.5 531 + GFF7446 0.36 -1.4 531 + GFF7446 0.36 -1.7 531 + GFF7446 0.36 +0.1 531 + GFF7446 0.36 +0.2 531 + GFF7446 0.36 -0.2 531 + GFF7446 0.36 +0.5 531 + GFF7446 0.36 -0.3 531 + GFF7446 0.36 +0.6 531 + GFF7446 0.36 -0.1 531 + GFF7446 0.36 -0.2 531 + GFF7446 0.36 -0.3 531 + GFF7446 0.36 -1.0 531 + GFF7446 0.36 -1.3 531 + GFF7446 0.36 +1.2 531 + GFF7446 0.36 +0.4 531 + GFF7446 0.36 -0.2 532 - GFF7446 0.36 +0.4 532 - GFF7446 0.36 -0.2 532 - GFF7446 0.36 +1.0 532 - GFF7446 0.36 +0.0 532 - GFF7446 0.36 -0.5 532 - GFF7446 0.36 +0.8 532 - GFF7446 0.36 +0.0 532 - GFF7446 0.36 -0.8 532 - GFF7446 0.36 -0.6 532 - GFF7446 0.36 -0.7 532 - GFF7446 0.36 +0.5 532 - GFF7446 0.36 +0.1 532 - GFF7446 0.36 -0.6 532 - GFF7446 0.36 -0.1 532 - GFF7446 0.36 +1.0 532 - GFF7446 0.36 -0.2 532 - GFF7446 0.36 +0.3 532 - GFF7446 0.36 +0.0 532 - GFF7446 0.36 +0.9 532 - GFF7446 0.36 -1.2 532 - GFF7446 0.36 +0.3 532 - GFF7446 0.36 -0.4 801 + GFF7446 0.56 -0.3 801 + GFF7446 0.56 -1.0 801 + GFF7446 0.56 -0.4 802 - GFF7446 0.56 +0.1 802 - GFF7446 0.56 -1.0 802 - GFF7446 0.56 -1.0 802 - GFF7446 0.56 -0.6 802 - GFF7446 0.56 -0.7 802 - GFF7446 0.56 -0.5 891 + GFF7446 0.63 -1.1 891 + GFF7446 0.63 -0.8 891 + GFF7446 0.63 +0.0 892 - GFF7446 0.63 -0.1 897 + GFF7446 0.63 -1.1 897 + GFF7446 0.63 -0.8 897 + GFF7446 0.63 -0.2 897 + GFF7446 0.63 -0.1 897 + GFF7446 0.63 -0.8 897 + GFF7446 0.63 -1.1 897 + GFF7446 0.63 -1.7 898 - GFF7446 0.63 -0.1 898 - GFF7446 0.63 -0.4 898 - GFF7446 0.63 +0.0 898 - GFF7446 0.63 +0.2 898 - GFF7446 0.63 +0.1 903 + GFF7446 0.64 -0.4 903 + GFF7446 0.64 -0.9 903 + GFF7446 0.64 -0.7 903 + GFF7446 0.64 -1.6 903 + GFF7446 0.64 +0.3 903 + GFF7446 0.64 -0.1 903 + GFF7446 0.64 -0.1 903 + GFF7446 0.64 +0.2 903 + GFF7446 0.64 -0.3 903 + GFF7446 0.64 +0.2 903 + GFF7446 0.64 +0.7 903 + GFF7446 0.64 -0.5 903 + GFF7446 0.64 +0.9 903 + GFF7446 0.64 -0.1 903 + GFF7446 0.64 -0.3 903 + GFF7446 0.64 +0.2 903 + GFF7446 0.64 -0.2 903 + GFF7446 0.64 +0.2 903 + GFF7446 0.64 -0.1 903 + GFF7446 0.64 +0.7 903 + GFF7446 0.64 +0.3 903 + GFF7446 0.64 -0.7 903 + GFF7446 0.64 -0.1 903 + GFF7446 0.64 +0.6 903 + GFF7446 0.64 -0.7 903 + GFF7446 0.64 +0.0 903 + GFF7446 0.64 +0.5 903 + GFF7446 0.64 +0.2 904 - GFF7446 0.64 +0.0 904 - GFF7446 0.64 +0.4 904 - GFF7446 0.64 +1.2 904 - GFF7446 0.64 +0.0 904 - GFF7446 0.64 -0.4 904 - GFF7446 0.64 +0.8 904 - GFF7446 0.64 +0.1 904 - GFF7446 0.64 +1.0 904 - GFF7446 0.64 -0.0 904 - GFF7446 0.64 -0.3 904 - GFF7446 0.64 -1.7 904 - GFF7446 0.64 -1.7 904 - GFF7446 0.64 -0.2 904 - GFF7446 0.64 +1.0 904 - GFF7446 0.64 -0.8 904 - GFF7446 0.64 -0.0 904 - GFF7446 0.64 -0.8 904 - GFF7446 0.64 +0.4 904 - GFF7446 0.64 -0.3 904 - GFF7446 0.64 -0.8 904 - GFF7446 0.64 +0.5 904 - GFF7446 0.64 -1.1 904 - GFF7446 0.64 +0.6 904 - GFF7446 0.64 +0.5 904 - GFF7446 0.64 -0.0 904 - GFF7446 0.64 +0.4 904 - GFF7446 0.64 +0.8 1,164 + GFF7446 0.83 +0.0 1,164 + GFF7446 0.83 +0.2 1,164 + GFF7446 0.83 +0.3 1,164 + GFF7446 0.83 -1.7 1,164 + GFF7446 0.83 +0.6 1,164 + GFF7446 0.83 +0.4 1,165 - GFF7446 0.83 -1.0 1,221 + GFF7446 0.88 +1.2 1,222 - GFF7446 0.88 -0.6 1,437 + +0.8 1,437 + +0.3 1,437 + +0.4 1,437 + -0.3 1,437 + +0.5 1,437 + -0.4 1,438 - +0.3 1,438 - -0.0 1,438 - +1.0
Or see this region's nucleotide sequence