Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7438 and GFF7439 are separated by 51 nucleotides GFF7439 and GFF7440 are separated by 133 nucleotides GFF7440 and GFF7441 are separated by 161 nucleotides
GFF7438 - no description, at 215 to 364
GFF7438
GFF7439 - no description, at 416 to 646
GFF7439
GFF7440 - no description, at 780 to 1,175
GFF7440
GFF7441 - Transposase, at 1,337 to 1,696
GFF7441
Position (kb)
0
1
2 Strain fitness (log2 ratio)
-2
-1
0
1 at 0.242 kb on + strand, within GFF7438 at 0.243 kb on - strand, within GFF7438 at 0.243 kb on - strand, within GFF7438 at 0.243 kb on - strand, within GFF7438 at 0.281 kb on - strand, within GFF7438 at 0.339 kb on + strand, within GFF7438 at 0.339 kb on + strand, within GFF7438 at 0.340 kb on - strand, within GFF7438 at 0.855 kb on + strand, within GFF7440 at 0.996 kb on + strand, within GFF7440 at 0.996 kb on + strand, within GFF7440 at 0.996 kb on + strand, within GFF7440 at 0.997 kb on - strand, within GFF7440 at 0.997 kb on - strand, within GFF7440 at 1.093 kb on - strand, within GFF7440 at 1.202 kb on + strand at 1.410 kb on + strand, within GFF7441 at 1.422 kb on + strand, within GFF7441 at 1.635 kb on - strand, within GFF7441 at 1.821 kb on + strand at 1.935 kb on + strand at 1.946 kb on + strand at 1.967 kb on + strand at 1.967 kb on + strand at 1.967 kb on + strand at 1.995 kb on + strand at 1.996 kb on - strand at 1.996 kb on - strand at 1.999 kb on + strand at 2.000 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 242 + GFF7438 0.18 +0.6 243 - GFF7438 0.19 +0.3 243 - GFF7438 0.19 +0.4 243 - GFF7438 0.19 -0.4 281 - GFF7438 0.44 -0.9 339 + GFF7438 0.83 +0.6 339 + GFF7438 0.83 +0.4 340 - GFF7438 0.83 +1.1 855 + GFF7440 0.19 +0.2 996 + GFF7440 0.55 -0.0 996 + GFF7440 0.55 -0.5 996 + GFF7440 0.55 -0.2 997 - GFF7440 0.55 +1.6 997 - GFF7440 0.55 -0.2 1,093 - GFF7440 0.79 +0.0 1,202 + +0.3 1,410 + GFF7441 0.20 -0.3 1,422 + GFF7441 0.24 +0.8 1,635 - GFF7441 0.83 +0.3 1,821 + +1.1 1,935 + -0.4 1,946 + -2.4 1,967 + -0.6 1,967 + -0.8 1,967 + -0.2 1,995 + +0.6 1,996 - -0.3 1,996 - -0.3 1,999 + -0.1 2,000 - +0.3
Or see this region's nucleotide sequence