Strain Fitness in Variovorax sp. SCN45 around GFF7428

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7428 and GFF7429 overlap by 4 nucleotidesGFF7429 and GFF7430 overlap by 4 nucleotides GFF7428 - Integrase/recombinase, RitC, at 1 to 462 GFF7428 GFF7429 - Integrase/recombinase, RitB, at 459 to 1,385 GFF7429 GFF7430 - Integrase/recombinase, RitA, at 1,382 to 2,065 GFF7430 Position (kb) 0 1Strain fitness (log2 ratio) -2 -1 0 1at 0.357 kb on + strand, within GFF7428at 0.358 kb on - strand, within GFF7428at 0.365 kb on + strand, within GFF7428at 0.378 kb on + strand, within GFF7428at 0.532 kb on + strandat 0.532 kb on + strandat 0.532 kb on + strandat 0.533 kb on - strandat 0.538 kb on + strandat 0.538 kb on + strandat 0.538 kb on + strandat 0.538 kb on + strandat 0.568 kb on + strand, within GFF7429at 0.568 kb on + strand, within GFF7429at 0.569 kb on - strand, within GFF7429at 0.569 kb on - strand, within GFF7429at 0.569 kb on - strand, within GFF7429at 0.569 kb on - strand, within GFF7429at 0.801 kb on + strand, within GFF7429at 0.802 kb on - strand, within GFF7429at 1.001 kb on - strand, within GFF7429at 1.077 kb on - strand, within GFF7429at 1.079 kb on - strand, within GFF7429at 1.079 kb on - strand, within GFF7429at 1.157 kb on + strand, within GFF7429at 1.158 kb on - strand, within GFF7429at 1.158 kb on - strand, within GFF7429at 1.222 kb on - strand, within GFF7429at 1.322 kb on - strandat 1.375 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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357 + GFF7428 0.77 +0.3
358 - GFF7428 0.77 -0.7
365 + GFF7428 0.79 +0.0
378 + GFF7428 0.82 -1.0
532 + -0.5
532 + +0.5
532 + -2.1
533 - -0.2
538 + +0.5
538 + -0.5
538 + -0.1
538 + +0.0
568 + GFF7429 0.12 +0.9
568 + GFF7429 0.12 +0.5
569 - GFF7429 0.12 -0.9
569 - GFF7429 0.12 -0.7
569 - GFF7429 0.12 -0.1
569 - GFF7429 0.12 +0.4
801 + GFF7429 0.37 -0.0
802 - GFF7429 0.37 -0.1
1,001 - GFF7429 0.58 -0.1
1,077 - GFF7429 0.67 -2.0
1,079 - GFF7429 0.67 +0.0
1,079 - GFF7429 0.67 -1.7
1,157 + GFF7429 0.75 -0.2
1,158 - GFF7429 0.75 -0.1
1,158 - GFF7429 0.75 +0.7
1,222 - GFF7429 0.82 -1.5
1,322 - +0.2
1,375 - -0.1

Or see this region's nucleotide sequence