Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7425 and GFF7426 overlap by 4 nucleotides GFF7426 and GFF7427 overlap by 4 nucleotides
GFF7425 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 202 to 933
GFF7425
GFF7426 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 930 to 2,843
GFF7426
GFF7427 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 2,840 to 3,868
GFF7427
Position (kb) 0
1
2
3 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 0.227 kb on + strand at 0.228 kb on - strand at 0.232 kb on - strand at 0.232 kb on - strand at 0.232 kb on - strand at 0.232 kb on - strand at 0.305 kb on + strand, within GFF7425 at 0.305 kb on + strand, within GFF7425 at 0.306 kb on - strand, within GFF7425 at 0.306 kb on - strand, within GFF7425 at 0.306 kb on - strand, within GFF7425 at 0.306 kb on - strand, within GFF7425 at 0.306 kb on - strand, within GFF7425 at 0.306 kb on - strand, within GFF7425 at 0.617 kb on + strand, within GFF7425 at 0.974 kb on - strand at 2.081 kb on - strand, within GFF7426 at 2.381 kb on - strand, within GFF7426 at 2.381 kb on - strand, within GFF7426 at 2.420 kb on - strand, within GFF7426 at 2.581 kb on + strand, within GFF7426 at 2.671 kb on + strand at 2.672 kb on - strand at 2.672 kb on - strand at 2.672 kb on - strand at 2.926 kb on - strand at 2.926 kb on - strand at 3.063 kb on + strand, within GFF7427 at 3.067 kb on - strand, within GFF7427 at 3.466 kb on - strand, within GFF7427 at 3.810 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 227 + -2.6 228 - -0.3 232 - +2.4 232 - -0.3 232 - -0.2 232 - -0.2 305 + GFF7425 0.14 -0.0 305 + GFF7425 0.14 +0.0 306 - GFF7425 0.14 +0.9 306 - GFF7425 0.14 +0.4 306 - GFF7425 0.14 +0.2 306 - GFF7425 0.14 -0.0 306 - GFF7425 0.14 +1.1 306 - GFF7425 0.14 -0.3 617 + GFF7425 0.57 -0.0 974 - -1.0 2,081 - GFF7426 0.60 +1.5 2,381 - GFF7426 0.76 -1.6 2,381 - GFF7426 0.76 -0.4 2,420 - GFF7426 0.78 +0.8 2,581 + GFF7426 0.86 -0.3 2,671 + -0.2 2,672 - -0.1 2,672 - +0.1 2,672 - -0.8 2,926 - +0.4 2,926 - +0.7 3,063 + GFF7427 0.22 +0.6 3,067 - GFF7427 0.22 +0.3 3,466 - GFF7427 0.61 -1.0 3,810 + +3.0
Or see this region's nucleotide sequence