Strain Fitness in Variovorax sp. SCN45 around GFF7423

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7421 and GFF7422 are separated by 0 nucleotidesGFF7422 and GFF7423 are separated by 46 nucleotidesGFF7423 and GFF7424 are separated by 226 nucleotides GFF7421 - Uncharacterized MFS-type transporter, at 2,078 to 3,238 GFF7421 GFF7422 - no description, at 3,239 to 3,421 GFF7422 GFF7423 - Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17), at 3,468 to 4,274 GFF7423 GFF7424 - no description, at 4,501 to 4,620 GFF7424 Position (kb) 3 4 5Strain fitness (log2 ratio) -1 0 1at 2.616 kb on + strand, within GFF7421at 2.721 kb on - strand, within GFF7421at 2.791 kb on + strand, within GFF7421at 2.901 kb on - strand, within GFF7421at 2.901 kb on - strand, within GFF7421at 2.979 kb on + strand, within GFF7421at 2.982 kb on + strand, within GFF7421at 3.039 kb on + strand, within GFF7421at 3.470 kb on - strandat 3.470 kb on - strandat 3.470 kb on - strandat 3.791 kb on - strand, within GFF7423at 4.078 kb on + strand, within GFF7423at 4.087 kb on - strand, within GFF7423at 4.184 kb on - strand, within GFF7423at 4.279 kb on + strandat 4.314 kb on - strandat 4.314 kb on - strandat 4.370 kb on + strandat 4.409 kb on + strandat 4.692 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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2,616 + GFF7421 0.46 +1.2
2,721 - GFF7421 0.55 -0.5
2,791 + GFF7421 0.61 -0.0
2,901 - GFF7421 0.71 +0.3
2,901 - GFF7421 0.71 +1.0
2,979 + GFF7421 0.78 -1.5
2,982 + GFF7421 0.78 -0.3
3,039 + GFF7421 0.83 -0.1
3,470 - -0.5
3,470 - +0.0
3,470 - +0.2
3,791 - GFF7423 0.40 +0.9
4,078 + GFF7423 0.76 -0.7
4,087 - GFF7423 0.77 -1.3
4,184 - GFF7423 0.89 -1.5
4,279 + +0.4
4,314 - -0.2
4,314 - -0.1
4,370 + -1.1
4,409 + -0.4
4,692 - -0.8

Or see this region's nucleotide sequence