Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF740 and GFF741 are separated by 38 nucleotides GFF741 and GFF742 are separated by 28 nucleotides GFF742 and GFF743 are separated by 294 nucleotides
GFF740 - Uncharacterized protein YdcJ, at 2,713 to 4,092
GFF740
GFF741 - Transcriptional regulator, LysR family, at 4,131 to 5,024
GFF741
GFF742 - D-2-hydroxyglutarate dehydrogenase (EC 1.1.99.2), at 5,053 to 6,471
GFF742
GFF743 - Hypothetical ABC transport system, periplasmic component, at 6,766 to 7,461
GFF743
Position (kb) 5
6
7 Strain fitness (log2 ratio) -2
-1
0
1 at 4.090 kb on + strand at 4.090 kb on + strand at 4.091 kb on - strand at 4.091 kb on - strand at 4.091 kb on - strand at 4.091 kb on - strand at 4.091 kb on - strand at 4.091 kb on - strand at 4.235 kb on + strand, within GFF741 at 4.235 kb on + strand, within GFF741 at 4.235 kb on + strand, within GFF741 at 4.236 kb on - strand, within GFF741 at 4.344 kb on + strand, within GFF741 at 4.344 kb on + strand, within GFF741 at 4.345 kb on - strand, within GFF741 at 4.345 kb on - strand, within GFF741 at 4.345 kb on - strand, within GFF741 at 4.345 kb on - strand, within GFF741 at 4.345 kb on - strand, within GFF741 at 4.345 kb on - strand, within GFF741 at 4.345 kb on - strand, within GFF741 at 4.378 kb on + strand, within GFF741 at 4.378 kb on + strand, within GFF741 at 4.379 kb on - strand, within GFF741 at 4.935 kb on + strand at 4.935 kb on + strand at 4.935 kb on + strand at 4.935 kb on + strand at 4.935 kb on + strand at 4.935 kb on + strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.936 kb on - strand at 4.939 kb on - strand at 4.939 kb on - strand at 5.891 kb on - strand, within GFF742 at 6.470 kb on - strand at 6.470 kb on - strand at 6.606 kb on + strand at 6.693 kb on + strand at 6.693 kb on + strand at 6.693 kb on + strand at 6.694 kb on - strand at 6.760 kb on + strand at 6.761 kb on - strand at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.907 kb on + strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 6.908 kb on - strand, within GFF743 at 7.119 kb on - strand, within GFF743 at 7.391 kb on + strand, within GFF743 at 7.391 kb on + strand, within GFF743 at 7.391 kb on + strand, within GFF743 at 7.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 4,090 + +0.2 4,090 + -0.3 4,091 - -0.8 4,091 - -0.7 4,091 - -0.2 4,091 - +0.1 4,091 - +0.1 4,091 - +0.4 4,235 + GFF741 0.12 -0.9 4,235 + GFF741 0.12 +1.4 4,235 + GFF741 0.12 -0.7 4,236 - GFF741 0.12 -0.4 4,344 + GFF741 0.24 -0.2 4,344 + GFF741 0.24 +0.2 4,345 - GFF741 0.24 -0.2 4,345 - GFF741 0.24 +0.4 4,345 - GFF741 0.24 -0.2 4,345 - GFF741 0.24 +0.4 4,345 - GFF741 0.24 -0.0 4,345 - GFF741 0.24 -1.2 4,345 - GFF741 0.24 -1.3 4,378 + GFF741 0.28 -0.5 4,378 + GFF741 0.28 -0.0 4,379 - GFF741 0.28 +0.2 4,935 + -0.8 4,935 + -0.2 4,935 + -0.2 4,935 + -0.6 4,935 + -0.1 4,935 + -0.2 4,936 - -0.0 4,936 - -0.6 4,936 - -0.5 4,936 - +0.4 4,936 - -0.5 4,936 - +0.3 4,936 - -0.2 4,936 - -0.1 4,936 - -0.7 4,936 - -0.7 4,936 - -0.3 4,939 - +0.3 4,939 - -0.1 5,891 - GFF742 0.59 +0.2 6,470 - -0.4 6,470 - -0.6 6,606 + -0.1 6,693 + -0.6 6,693 + +0.8 6,693 + -0.3 6,694 - +0.7 6,760 + -0.4 6,761 - +0.0 6,907 + GFF743 0.20 -0.7 6,907 + GFF743 0.20 -1.2 6,907 + GFF743 0.20 -2.1 6,907 + GFF743 0.20 -0.7 6,907 + GFF743 0.20 +0.9 6,908 - GFF743 0.20 +0.1 6,908 - GFF743 0.20 +0.4 6,908 - GFF743 0.20 -0.6 6,908 - GFF743 0.20 -0.3 6,908 - GFF743 0.20 -1.5 6,908 - GFF743 0.20 -0.1 7,119 - GFF743 0.51 -0.4 7,391 + GFF743 0.90 -0.9 7,391 + GFF743 0.90 -0.5 7,391 + GFF743 0.90 -0.4 7,392 - +0.0
Or see this region's nucleotide sequence