Strain Fitness in Variovorax sp. SCN45 around GFF7404

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7403 and GFF7404 are separated by 37 nucleotidesGFF7404 and GFF7405 are separated by 193 nucleotides GFF7403 - hypothetical protein, at 255 to 524 GFF7403 GFF7404 - hypothetical protein, at 562 to 1,191 GFF7404 GFF7405 - BUG/TctC family periplasmic protein, at 1,385 to 2,407 GFF7405 Position (kb) 0 1 2Strain fitness (log2 ratio) -1 0 1at 0.209 kb on + strandat 0.210 kb on - strandat 0.274 kb on + strandat 0.431 kb on + strand, within GFF7403at 0.523 kb on - strandat 0.600 kb on - strandat 0.758 kb on - strand, within GFF7404at 0.843 kb on + strand, within GFF7404at 0.844 kb on - strand, within GFF7404at 0.940 kb on + strand, within GFF7404at 1.214 kb on + strandat 1.326 kb on - strandat 1.326 kb on - strandat 1.472 kb on - strandat 1.597 kb on - strand, within GFF7405at 1.677 kb on + strand, within GFF7405at 1.815 kb on + strand, within GFF7405at 1.816 kb on - strand, within GFF7405at 1.816 kb on - strand, within GFF7405at 1.816 kb on - strand, within GFF7405at 1.932 kb on + strand, within GFF7405at 1.933 kb on - strand, within GFF7405at 2.032 kb on - strand, within GFF7405at 2.094 kb on + strand, within GFF7405at 2.094 kb on + strand, within GFF7405

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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209 + +0.3
210 - -0.3
274 + -0.1
431 + GFF7403 0.65 -0.3
523 - +0.0
600 - +0.6
758 - GFF7404 0.31 +0.1
843 + GFF7404 0.45 -0.5
844 - GFF7404 0.45 -0.1
940 + GFF7404 0.60 -0.1
1,214 + -1.1
1,326 - -0.7
1,326 - -0.1
1,472 - +1.0
1,597 - GFF7405 0.21 +0.2
1,677 + GFF7405 0.29 +0.2
1,815 + GFF7405 0.42 +1.3
1,816 - GFF7405 0.42 -0.0
1,816 - GFF7405 0.42 +0.3
1,816 - GFF7405 0.42 -0.4
1,932 + GFF7405 0.53 +0.1
1,933 - GFF7405 0.54 +0.7
2,032 - GFF7405 0.63 -0.4
2,094 + GFF7405 0.69 -0.3
2,094 + GFF7405 0.69 +1.7

Or see this region's nucleotide sequence