Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7360 and GFF7361 are separated by 67 nucleotides GFF7361 and GFF7362 overlap by 4 nucleotides GFF7362 and GFF7363 are separated by 195 nucleotides
GFF7360 - Urea ABC transporter, substrate-binding protein UrtA, at 7,761 to 8,900
GFF7360
GFF7361 - Acyl-coenzyme A thioesterase PaaD (Pse.pu.) (E. coli PaaI), at 8,968 to 9,402
GFF7361
GFF7362 - Enoyl-CoA hydratase (EC 4.2.1.17), at 9,399 to 10,196
GFF7362
GFF7363 - hypothetical protein, at 10,392 to 11,363
GFF7363
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 8.426 kb on - strand, within GFF7360 at 8.627 kb on + strand, within GFF7360 at 8.628 kb on - strand, within GFF7360 at 8.690 kb on - strand, within GFF7360 at 8.694 kb on + strand, within GFF7360 at 8.749 kb on + strand, within GFF7360 at 8.749 kb on + strand, within GFF7360 at 8.843 kb on - strand at 8.913 kb on + strand at 8.914 kb on - strand at 8.917 kb on - strand at 8.969 kb on + strand at 9.059 kb on + strand, within GFF7361 at 9.106 kb on - strand, within GFF7361 at 9.106 kb on - strand, within GFF7361 at 9.106 kb on - strand, within GFF7361 at 9.106 kb on - strand, within GFF7361 at 9.106 kb on - strand, within GFF7361 at 9.106 kb on - strand, within GFF7361 at 9.108 kb on - strand, within GFF7361 at 9.108 kb on - strand, within GFF7361 at 9.469 kb on + strand at 9.937 kb on + strand, within GFF7362 at 9.937 kb on + strand, within GFF7362 at 10.179 kb on + strand at 10.179 kb on + strand at 10.180 kb on - strand at 10.363 kb on - strand at 10.367 kb on - strand at 10.390 kb on + strand at 10.390 kb on + strand at 10.390 kb on + strand at 10.390 kb on + strand at 10.391 kb on - strand at 10.391 kb on - strand at 10.391 kb on - strand at 10.648 kb on + strand, within GFF7363 at 10.648 kb on + strand, within GFF7363 at 10.683 kb on + strand, within GFF7363 at 10.683 kb on + strand, within GFF7363 at 10.683 kb on + strand, within GFF7363 at 10.683 kb on + strand, within GFF7363 at 10.684 kb on - strand, within GFF7363 at 10.704 kb on + strand, within GFF7363 at 10.719 kb on + strand, within GFF7363 at 10.720 kb on - strand, within GFF7363 at 10.720 kb on - strand, within GFF7363 at 10.800 kb on + strand, within GFF7363 at 10.819 kb on - strand, within GFF7363 at 10.857 kb on + strand, within GFF7363 at 10.857 kb on + strand, within GFF7363 at 10.857 kb on + strand, within GFF7363 at 10.857 kb on + strand, within GFF7363 at 10.857 kb on + strand, within GFF7363 at 10.857 kb on + strand, within GFF7363 at 10.859 kb on + strand, within GFF7363 at 10.860 kb on - strand, within GFF7363
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 8,426 - GFF7360 0.58 +0.2 8,627 + GFF7360 0.76 +0.6 8,628 - GFF7360 0.76 +1.5 8,690 - GFF7360 0.81 -0.7 8,694 + GFF7360 0.82 +0.0 8,749 + GFF7360 0.87 +0.4 8,749 + GFF7360 0.87 +0.0 8,843 - -0.3 8,913 + -0.4 8,914 - +1.5 8,917 - +0.5 8,969 + -0.4 9,059 + GFF7361 0.21 +0.2 9,106 - GFF7361 0.32 +0.7 9,106 - GFF7361 0.32 -0.0 9,106 - GFF7361 0.32 -1.1 9,106 - GFF7361 0.32 -0.3 9,106 - GFF7361 0.32 +1.8 9,106 - GFF7361 0.32 +1.1 9,108 - GFF7361 0.32 +0.3 9,108 - GFF7361 0.32 -1.0 9,469 + +0.7 9,937 + GFF7362 0.67 -0.1 9,937 + GFF7362 0.67 -0.9 10,179 + +0.5 10,179 + +1.3 10,180 - +0.4 10,363 - -0.3 10,367 - -0.1 10,390 + -2.0 10,390 + -0.4 10,390 + +0.5 10,390 + -0.5 10,391 - -1.2 10,391 - +1.1 10,391 - +0.0 10,648 + GFF7363 0.26 +0.1 10,648 + GFF7363 0.26 +1.1 10,683 + GFF7363 0.30 -0.2 10,683 + GFF7363 0.30 +0.1 10,683 + GFF7363 0.30 -0.2 10,683 + GFF7363 0.30 -0.6 10,684 - GFF7363 0.30 -0.4 10,704 + GFF7363 0.32 +0.3 10,719 + GFF7363 0.34 -0.5 10,720 - GFF7363 0.34 -0.5 10,720 - GFF7363 0.34 +0.5 10,800 + GFF7363 0.42 -1.4 10,819 - GFF7363 0.44 +0.5 10,857 + GFF7363 0.48 -0.6 10,857 + GFF7363 0.48 -0.4 10,857 + GFF7363 0.48 +1.5 10,857 + GFF7363 0.48 -0.6 10,857 + GFF7363 0.48 +0.8 10,857 + GFF7363 0.48 +0.2 10,859 + GFF7363 0.48 +1.2 10,860 - GFF7363 0.48 +1.6
Or see this region's nucleotide sequence