Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7249 and GFF7250 are separated by 95 nucleotides GFF7250 and GFF7251 are separated by 350 nucleotides GFF7251 and GFF7252 overlap by 7 nucleotides GFF7252 and GFF7253 are separated by 1 nucleotides
GFF7249 - no description, at 28,560 to 28,865
GFF7249
GFF7250 - Putative transposase InsK for insertion sequence element IS150, at 28,961 to 29,134
GFF7250
GFF7251 - Mobile element protein, at 29,485 to 29,748
GFF7251
GFF7252 - Mobile element protein, at 29,742 to 30,557
GFF7252
GFF7253 - Mobile element protein, at 30,559 to 30,807
GFF7253
Position (kb) 29
30
31 Strain fitness (log2 ratio) -1
0
1
2 at 28.841 kb on + strand at 28.863 kb on + strand at 28.864 kb on - strand at 28.864 kb on - strand at 28.897 kb on + strand at 28.898 kb on - strand at 28.907 kb on + strand at 28.907 kb on + strand at 28.954 kb on + strand at 28.955 kb on - strand at 29.054 kb on + strand, within GFF7250 at 29.133 kb on - strand at 29.301 kb on - strand at 29.596 kb on + strand, within GFF7251 at 29.596 kb on + strand, within GFF7251 at 29.597 kb on - strand, within GFF7251 at 30.493 kb on - strand at 30.603 kb on - strand, within GFF7253 at 30.632 kb on + strand, within GFF7253 at 30.749 kb on + strand, within GFF7253 at 30.749 kb on + strand, within GFF7253 at 30.750 kb on - strand, within GFF7253 at 30.785 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 28,841 + +0.7 28,863 + +1.1 28,864 - +0.1 28,864 - -0.7 28,897 + +2.1 28,898 - +0.1 28,907 + +0.3 28,907 + +0.2 28,954 + -0.4 28,955 - +0.4 29,054 + GFF7250 0.53 -0.5 29,133 - -0.1 29,301 - -0.6 29,596 + GFF7251 0.42 -0.3 29,596 + GFF7251 0.42 -0.4 29,597 - GFF7251 0.42 -0.2 30,493 - +0.3 30,603 - GFF7253 0.18 +0.1 30,632 + GFF7253 0.29 -1.0 30,749 + GFF7253 0.76 +2.3 30,749 + GFF7253 0.76 -1.0 30,750 - GFF7253 0.77 -0.5 30,785 + +0.1
Or see this region's nucleotide sequence