Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7223 and GFF7224 are separated by 35 nucleotides GFF7224 and GFF7225 are separated by 23 nucleotides
GFF7223 - Fatty acid desaturase (EC 1.14.19.1); Delta-9 fatty acid desaturase (EC 1.14.19.1), at 4,815 to 5,999
GFF7223
GFF7224 - hypothetical protein, at 6,035 to 6,424
GFF7224
GFF7225 - hypothetical protein, at 6,448 to 7,422
GFF7225
Position (kb)
6
7 Strain fitness (log2 ratio)
-2
-1
0
1 at 5.127 kb on + strand, within GFF7223 at 5.127 kb on + strand, within GFF7223 at 5.127 kb on + strand, within GFF7223 at 5.128 kb on - strand, within GFF7223 at 5.128 kb on - strand, within GFF7223 at 5.128 kb on - strand, within GFF7223 at 5.128 kb on - strand, within GFF7223 at 5.130 kb on + strand, within GFF7223 at 5.212 kb on - strand, within GFF7223 at 5.341 kb on + strand, within GFF7223 at 5.341 kb on + strand, within GFF7223 at 5.359 kb on - strand, within GFF7223 at 5.517 kb on + strand, within GFF7223 at 5.517 kb on + strand, within GFF7223 at 5.517 kb on + strand, within GFF7223 at 5.517 kb on + strand, within GFF7223 at 5.618 kb on - strand, within GFF7223 at 5.640 kb on + strand, within GFF7223 at 5.641 kb on - strand, within GFF7223 at 5.658 kb on + strand, within GFF7223 at 5.658 kb on + strand, within GFF7223 at 5.659 kb on - strand, within GFF7223 at 5.659 kb on - strand, within GFF7223 at 5.659 kb on - strand, within GFF7223 at 5.844 kb on + strand, within GFF7223 at 5.844 kb on + strand, within GFF7223 at 5.845 kb on - strand, within GFF7223 at 5.845 kb on - strand, within GFF7223 at 5.991 kb on + strand at 5.991 kb on + strand at 5.991 kb on + strand at 5.992 kb on - strand at 6.826 kb on + strand, within GFF7225 at 6.826 kb on + strand, within GFF7225 at 6.826 kb on + strand, within GFF7225 at 6.827 kb on - strand, within GFF7225 at 6.928 kb on + strand, within GFF7225 at 6.928 kb on + strand, within GFF7225 at 6.929 kb on - strand, within GFF7225 at 6.929 kb on - strand, within GFF7225 at 6.929 kb on - strand, within GFF7225 at 6.929 kb on - strand, within GFF7225 at 6.929 kb on - strand, within GFF7225 at 6.929 kb on - strand, within GFF7225 at 6.929 kb on - strand, within GFF7225 at 6.982 kb on + strand, within GFF7225 at 6.983 kb on - strand, within GFF7225 at 7.240 kb on + strand, within GFF7225
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 5,127 + GFF7223 0.26 -0.1 5,127 + GFF7223 0.26 -0.4 5,127 + GFF7223 0.26 -1.8 5,128 - GFF7223 0.26 -0.1 5,128 - GFF7223 0.26 +0.3 5,128 - GFF7223 0.26 -1.1 5,128 - GFF7223 0.26 -0.1 5,130 + GFF7223 0.27 -0.4 5,212 - GFF7223 0.34 -1.1 5,341 + GFF7223 0.44 +0.6 5,341 + GFF7223 0.44 +0.8 5,359 - GFF7223 0.46 -0.7 5,517 + GFF7223 0.59 -0.5 5,517 + GFF7223 0.59 -0.2 5,517 + GFF7223 0.59 -0.6 5,517 + GFF7223 0.59 -0.7 5,618 - GFF7223 0.68 +1.7 5,640 + GFF7223 0.70 -0.4 5,641 - GFF7223 0.70 +0.1 5,658 + GFF7223 0.71 -1.7 5,658 + GFF7223 0.71 +1.7 5,659 - GFF7223 0.71 -0.2 5,659 - GFF7223 0.71 -0.9 5,659 - GFF7223 0.71 +0.1 5,844 + GFF7223 0.87 -0.0 5,844 + GFF7223 0.87 +1.0 5,845 - GFF7223 0.87 -0.8 5,845 - GFF7223 0.87 -2.0 5,991 + -0.4 5,991 + +0.7 5,991 + +0.2 5,992 - +0.4 6,826 + GFF7225 0.39 +0.3 6,826 + GFF7225 0.39 +1.1 6,826 + GFF7225 0.39 -1.0 6,827 - GFF7225 0.39 -0.9 6,928 + GFF7225 0.49 +0.1 6,928 + GFF7225 0.49 -0.1 6,929 - GFF7225 0.49 -0.4 6,929 - GFF7225 0.49 -0.8 6,929 - GFF7225 0.49 +0.9 6,929 - GFF7225 0.49 +1.3 6,929 - GFF7225 0.49 +0.7 6,929 - GFF7225 0.49 -0.9 6,929 - GFF7225 0.49 -0.5 6,982 + GFF7225 0.55 -0.2 6,983 - GFF7225 0.55 -0.3 7,240 + GFF7225 0.81 -0.3
Or see this region's nucleotide sequence