Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7117 and GFF7118 overlap by 4 nucleotides GFF7118 and GFF7119 are separated by 6 nucleotides GFF7119 and GFF7120 are separated by 1 nucleotides
GFF7117 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 35,409 to 36,239
GFF7117
GFF7118 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 36,236 to 37,177
GFF7118
GFF7119 - Ortho-halobenzoate 1,2-dioxygenase beta-ISP protein OhbA, at 37,184 to 37,660
GFF7119
GFF7120 - Ortho-halobenzoate 1,2-dioxygenase alpha-ISP protein OhbB, at 37,662 to 38,906
GFF7120
Position (kb)
37
38 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.186 kb on + strand at 36.187 kb on - strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.230 kb on + strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.231 kb on - strand at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.447 kb on + strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.448 kb on - strand, within GFF7118 at 36.921 kb on - strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.924 kb on + strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.925 kb on - strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.972 kb on + strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 36.973 kb on - strand, within GFF7118 at 37.032 kb on + strand, within GFF7118 at 37.185 kb on + strand at 37.185 kb on + strand at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.290 kb on + strand, within GFF7119 at 37.291 kb on - strand, within GFF7119 at 37.291 kb on - strand, within GFF7119 at 37.291 kb on - strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.431 kb on + strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.432 kb on - strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.491 kb on + strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.492 kb on - strand, within GFF7119 at 37.545 kb on + strand, within GFF7119 at 37.546 kb on - strand, within GFF7119 at 37.546 kb on - strand, within GFF7119 at 37.567 kb on - strand, within GFF7119 at 37.567 kb on - strand, within GFF7119 at 37.611 kb on + strand, within GFF7119 at 37.611 kb on + strand, within GFF7119 at 37.611 kb on + strand, within GFF7119 at 37.638 kb on + strand at 37.638 kb on + strand at 37.639 kb on - strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.675 kb on + strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.676 kb on - strand at 37.684 kb on + strand at 37.685 kb on - strand at 37.685 kb on - strand at 37.840 kb on + strand, within GFF7120 at 37.840 kb on + strand, within GFF7120 at 37.840 kb on + strand, within GFF7120 at 37.840 kb on + strand, within GFF7120 at 37.841 kb on - strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.918 kb on + strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 37.919 kb on - strand, within GFF7120 at 38.092 kb on - strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.119 kb on + strand, within GFF7120 at 38.120 kb on - strand, within GFF7120 at 38.120 kb on - strand, within GFF7120 at 38.120 kb on - strand, within GFF7120 at 38.269 kb on + strand, within GFF7120 at 38.269 kb on + strand, within GFF7120 at 38.269 kb on + strand, within GFF7120 at 38.269 kb on + strand, within GFF7120 at 38.269 kb on + strand, within GFF7120 at 38.270 kb on - strand, within GFF7120 at 38.270 kb on - strand, within GFF7120 at 38.270 kb on - strand, within GFF7120 at 38.270 kb on - strand, within GFF7120 at 38.270 kb on - strand, within GFF7120 at 38.270 kb on - strand, within GFF7120 at 38.270 kb on - strand, within GFF7120 at 38.294 kb on - strand, within GFF7120 at 38.294 kb on - strand, within GFF7120 at 38.294 kb on - strand, within GFF7120 at 38.294 kb on - strand, within GFF7120 at 38.395 kb on + strand, within GFF7120 at 38.395 kb on + strand, within GFF7120 at 38.395 kb on + strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.396 kb on - strand, within GFF7120 at 38.440 kb on - strand, within GFF7120 at 38.440 kb on - strand, within GFF7120 at 38.440 kb on - strand, within GFF7120 at 38.569 kb on + strand, within GFF7120 at 38.569 kb on + strand, within GFF7120 at 38.570 kb on - strand, within GFF7120 at 38.585 kb on - strand, within GFF7120 at 38.585 kb on - strand, within GFF7120 at 38.585 kb on - strand, within GFF7120
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 36,186 + +0.6 36,186 + -1.3 36,186 + -1.1 36,186 + -0.4 36,187 - -0.4 36,230 + +0.6 36,230 + +0.3 36,230 + -0.2 36,230 + -0.0 36,230 + -0.4 36,230 + -0.0 36,231 - +0.0 36,231 - -0.5 36,231 - +0.1 36,447 + GFF7118 0.22 +0.2 36,447 + GFF7118 0.22 -0.6 36,447 + GFF7118 0.22 +1.4 36,448 - GFF7118 0.23 -0.8 36,448 - GFF7118 0.23 +0.3 36,448 - GFF7118 0.23 -1.4 36,921 - GFF7118 0.73 +0.4 36,924 + GFF7118 0.73 -0.5 36,924 + GFF7118 0.73 -0.2 36,924 + GFF7118 0.73 -1.5 36,925 - GFF7118 0.73 -1.9 36,925 - GFF7118 0.73 +1.0 36,925 - GFF7118 0.73 -0.2 36,925 - GFF7118 0.73 +0.3 36,925 - GFF7118 0.73 +0.9 36,925 - GFF7118 0.73 +0.2 36,925 - GFF7118 0.73 +1.5 36,925 - GFF7118 0.73 +0.1 36,925 - GFF7118 0.73 -0.2 36,972 + GFF7118 0.78 +1.2 36,972 + GFF7118 0.78 -1.0 36,972 + GFF7118 0.78 -1.2 36,972 + GFF7118 0.78 +0.4 36,973 - GFF7118 0.78 -0.2 36,973 - GFF7118 0.78 +0.4 37,032 + GFF7118 0.85 +0.6 37,185 + +0.9 37,185 + +0.4 37,290 + GFF7119 0.22 -0.8 37,290 + GFF7119 0.22 -0.8 37,290 + GFF7119 0.22 +1.3 37,290 + GFF7119 0.22 +0.3 37,290 + GFF7119 0.22 -1.1 37,290 + GFF7119 0.22 +0.4 37,291 - GFF7119 0.22 -0.2 37,291 - GFF7119 0.22 -0.5 37,291 - GFF7119 0.22 +1.4 37,431 + GFF7119 0.52 +0.7 37,431 + GFF7119 0.52 +0.1 37,431 + GFF7119 0.52 +0.0 37,431 + GFF7119 0.52 -1.4 37,431 + GFF7119 0.52 +0.3 37,431 + GFF7119 0.52 -1.2 37,431 + GFF7119 0.52 -0.5 37,431 + GFF7119 0.52 -0.1 37,432 - GFF7119 0.52 -0.8 37,432 - GFF7119 0.52 -1.4 37,432 - GFF7119 0.52 -2.5 37,432 - GFF7119 0.52 -0.6 37,432 - GFF7119 0.52 +0.2 37,432 - GFF7119 0.52 +1.3 37,432 - GFF7119 0.52 +0.3 37,432 - GFF7119 0.52 -0.7 37,491 + GFF7119 0.64 -0.1 37,491 + GFF7119 0.64 +0.3 37,491 + GFF7119 0.64 +0.0 37,491 + GFF7119 0.64 +0.1 37,491 + GFF7119 0.64 +1.0 37,491 + GFF7119 0.64 +0.5 37,491 + GFF7119 0.64 -0.1 37,491 + GFF7119 0.64 +0.2 37,491 + GFF7119 0.64 +0.1 37,491 + GFF7119 0.64 +0.1 37,491 + GFF7119 0.64 -0.5 37,491 + GFF7119 0.64 -0.0 37,491 + GFF7119 0.64 -0.5 37,491 + GFF7119 0.64 +0.6 37,491 + GFF7119 0.64 -0.3 37,491 + GFF7119 0.64 -0.1 37,491 + GFF7119 0.64 +0.1 37,491 + GFF7119 0.64 -0.6 37,492 - GFF7119 0.65 -0.2 37,492 - GFF7119 0.65 +0.2 37,492 - GFF7119 0.65 -0.3 37,492 - GFF7119 0.65 +0.1 37,492 - GFF7119 0.65 -0.8 37,492 - GFF7119 0.65 +0.3 37,492 - GFF7119 0.65 -0.3 37,492 - GFF7119 0.65 -0.6 37,492 - GFF7119 0.65 -1.0 37,492 - GFF7119 0.65 +0.2 37,492 - GFF7119 0.65 +0.6 37,492 - GFF7119 0.65 -0.4 37,492 - GFF7119 0.65 +0.4 37,492 - GFF7119 0.65 +0.1 37,492 - GFF7119 0.65 -0.6 37,492 - GFF7119 0.65 +0.1 37,492 - GFF7119 0.65 -1.8 37,492 - GFF7119 0.65 +0.0 37,492 - GFF7119 0.65 -0.2 37,492 - GFF7119 0.65 -0.7 37,492 - GFF7119 0.65 -0.1 37,545 + GFF7119 0.76 -0.0 37,546 - GFF7119 0.76 +0.8 37,546 - GFF7119 0.76 -1.2 37,567 - GFF7119 0.80 -0.7 37,567 - GFF7119 0.80 +0.5 37,611 + GFF7119 0.90 -1.1 37,611 + GFF7119 0.90 +3.4 37,611 + GFF7119 0.90 -0.9 37,638 + +0.5 37,638 + -0.7 37,639 - -1.5 37,675 + +0.4 37,675 + -0.5 37,675 + +0.2 37,675 + -0.3 37,675 + +0.0 37,675 + +0.5 37,675 + -1.0 37,675 + -0.1 37,675 + +0.0 37,675 + +0.5 37,676 - -0.0 37,676 - +0.6 37,676 - +0.7 37,676 - -0.2 37,676 - +1.1 37,676 - +2.1 37,676 - +2.2 37,676 - +0.5 37,676 - -0.5 37,676 - -0.4 37,676 - -0.4 37,684 + +0.5 37,685 - -1.1 37,685 - +0.0 37,840 + GFF7120 0.14 -0.4 37,840 + GFF7120 0.14 +0.4 37,840 + GFF7120 0.14 +0.4 37,840 + GFF7120 0.14 -0.0 37,841 - GFF7120 0.14 -1.3 37,918 + GFF7120 0.21 -0.0 37,918 + GFF7120 0.21 -0.4 37,918 + GFF7120 0.21 -0.6 37,918 + GFF7120 0.21 +0.4 37,918 + GFF7120 0.21 +0.3 37,918 + GFF7120 0.21 -0.6 37,919 - GFF7120 0.21 -0.1 37,919 - GFF7120 0.21 +0.1 37,919 - GFF7120 0.21 +0.0 37,919 - GFF7120 0.21 -1.1 38,092 - GFF7120 0.35 -0.2 38,119 + GFF7120 0.37 -0.1 38,119 + GFF7120 0.37 +0.4 38,119 + GFF7120 0.37 -1.3 38,119 + GFF7120 0.37 -0.4 38,120 - GFF7120 0.37 -1.0 38,120 - GFF7120 0.37 +0.6 38,120 - GFF7120 0.37 -0.4 38,269 + GFF7120 0.49 -0.6 38,269 + GFF7120 0.49 +0.4 38,269 + GFF7120 0.49 -0.2 38,269 + GFF7120 0.49 -0.1 38,269 + GFF7120 0.49 +0.5 38,270 - GFF7120 0.49 -1.9 38,270 - GFF7120 0.49 -2.0 38,270 - GFF7120 0.49 +0.4 38,270 - GFF7120 0.49 +2.4 38,270 - GFF7120 0.49 +0.0 38,270 - GFF7120 0.49 +0.7 38,270 - GFF7120 0.49 +0.4 38,294 - GFF7120 0.51 -0.4 38,294 - GFF7120 0.51 +1.6 38,294 - GFF7120 0.51 +0.5 38,294 - GFF7120 0.51 +0.2 38,395 + GFF7120 0.59 +0.2 38,395 + GFF7120 0.59 +1.3 38,395 + GFF7120 0.59 +1.0 38,396 - GFF7120 0.59 +0.5 38,396 - GFF7120 0.59 +2.0 38,396 - GFF7120 0.59 +0.5 38,396 - GFF7120 0.59 +0.1 38,396 - GFF7120 0.59 -0.0 38,396 - GFF7120 0.59 -0.6 38,396 - GFF7120 0.59 -1.0 38,396 - GFF7120 0.59 -1.0 38,440 - GFF7120 0.62 -0.9 38,440 - GFF7120 0.62 -0.3 38,440 - GFF7120 0.62 -0.7 38,569 + GFF7120 0.73 -0.2 38,569 + GFF7120 0.73 -0.5 38,570 - GFF7120 0.73 -0.5 38,585 - GFF7120 0.74 +0.9 38,585 - GFF7120 0.74 +0.1 38,585 - GFF7120 0.74 +0.5
Or see this region's nucleotide sequence