Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7064 and GFF7065 are separated by 68 nucleotides GFF7065 and GFF7066 overlap by 4 nucleotides GFF7066 and GFF7067 are separated by 132 nucleotides
GFF7064 - Glutamate--cysteine ligase (EC 6.3.2.2), at 22,571 to 24,076
GFF7064
GFF7065 - no description, at 24,145 to 24,279
GFF7065
GFF7066 - Two-component transcriptional response regulator receiver domain, at 24,276 to 24,656
GFF7066
GFF7067 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 24,789 to 25,718
GFF7067
Position (kb) 24
25 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 23.279 kb on + strand, within GFF7064 at 23.279 kb on + strand, within GFF7064 at 23.280 kb on - strand, within GFF7064 at 23.315 kb on + strand, within GFF7064 at 23.316 kb on - strand, within GFF7064 at 23.316 kb on - strand, within GFF7064 at 23.316 kb on - strand, within GFF7064 at 23.360 kb on + strand, within GFF7064 at 23.360 kb on + strand, within GFF7064 at 23.360 kb on + strand, within GFF7064 at 23.360 kb on + strand, within GFF7064 at 23.360 kb on + strand, within GFF7064 at 23.360 kb on + strand, within GFF7064 at 23.361 kb on - strand, within GFF7064 at 23.361 kb on - strand, within GFF7064 at 23.438 kb on + strand, within GFF7064 at 23.439 kb on - strand, within GFF7064 at 23.439 kb on - strand, within GFF7064 at 23.843 kb on + strand, within GFF7064 at 24.005 kb on + strand at 24.005 kb on + strand at 24.005 kb on + strand at 24.005 kb on + strand at 24.006 kb on - strand at 24.006 kb on - strand at 24.017 kb on + strand at 24.017 kb on + strand at 24.017 kb on + strand at 24.017 kb on + strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.018 kb on - strand at 24.074 kb on + strand at 24.075 kb on - strand at 24.151 kb on + strand at 24.151 kb on + strand at 24.152 kb on - strand at 24.152 kb on - strand at 24.241 kb on - strand, within GFF7065 at 24.291 kb on + strand at 24.291 kb on + strand at 24.292 kb on - strand at 24.292 kb on - strand at 24.534 kb on + strand, within GFF7066 at 24.534 kb on + strand, within GFF7066 at 24.534 kb on + strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.535 kb on - strand, within GFF7066 at 24.624 kb on + strand at 24.624 kb on + strand at 24.625 kb on - strand at 24.770 kb on - strand at 25.233 kb on + strand, within GFF7067 at 25.272 kb on + strand, within GFF7067 at 25.296 kb on + strand, within GFF7067 at 25.296 kb on + strand, within GFF7067 at 25.296 kb on + strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.297 kb on - strand, within GFF7067 at 25.428 kb on + strand, within GFF7067
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 23,279 + GFF7064 0.47 -0.2 23,279 + GFF7064 0.47 -1.2 23,280 - GFF7064 0.47 -0.5 23,315 + GFF7064 0.49 +1.4 23,316 - GFF7064 0.49 -0.4 23,316 - GFF7064 0.49 +0.1 23,316 - GFF7064 0.49 -1.6 23,360 + GFF7064 0.52 -0.8 23,360 + GFF7064 0.52 -0.3 23,360 + GFF7064 0.52 +0.5 23,360 + GFF7064 0.52 -0.0 23,360 + GFF7064 0.52 -0.0 23,360 + GFF7064 0.52 -0.3 23,361 - GFF7064 0.52 -0.8 23,361 - GFF7064 0.52 -0.6 23,438 + GFF7064 0.58 -0.0 23,439 - GFF7064 0.58 -0.8 23,439 - GFF7064 0.58 -0.8 23,843 + GFF7064 0.84 +0.5 24,005 + +0.2 24,005 + +0.4 24,005 + +0.1 24,005 + -0.5 24,006 - -0.5 24,006 - +0.4 24,017 + +0.1 24,017 + +0.4 24,017 + -1.9 24,017 + +0.2 24,018 - +0.3 24,018 - -0.5 24,018 - -0.1 24,018 - -0.4 24,018 - +0.3 24,018 - -0.1 24,018 - -0.3 24,018 - +0.4 24,018 - +0.2 24,018 - +0.1 24,018 - -0.2 24,018 - -0.2 24,074 + +0.0 24,075 - +0.2 24,151 + +1.0 24,151 + -0.2 24,152 - +0.2 24,152 - -0.9 24,241 - GFF7065 0.71 -2.8 24,291 + +0.1 24,291 + +0.6 24,292 - +0.0 24,292 - -0.4 24,534 + GFF7066 0.68 -0.0 24,534 + GFF7066 0.68 +0.7 24,534 + GFF7066 0.68 -0.0 24,535 - GFF7066 0.68 +0.2 24,535 - GFF7066 0.68 +0.3 24,535 - GFF7066 0.68 +0.8 24,535 - GFF7066 0.68 -0.2 24,535 - GFF7066 0.68 -0.5 24,535 - GFF7066 0.68 +0.1 24,535 - GFF7066 0.68 -0.9 24,624 + +0.5 24,624 + -0.2 24,625 - +0.1 24,770 - -0.5 25,233 + GFF7067 0.48 -1.7 25,272 + GFF7067 0.52 +0.9 25,296 + GFF7067 0.55 -0.3 25,296 + GFF7067 0.55 -1.1 25,296 + GFF7067 0.55 +0.2 25,297 - GFF7067 0.55 +0.5 25,297 - GFF7067 0.55 +0.6 25,297 - GFF7067 0.55 +1.1 25,297 - GFF7067 0.55 -0.3 25,428 + GFF7067 0.69 +1.1
Or see this region's nucleotide sequence