Strain Fitness in Variovorax sp. SCN45 around GFF7015

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7014 and GFF7015 are separated by 181 nucleotidesGFF7015 and GFF7016 are separated by 43 nucleotides GFF7014 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 29,533 to 31,068 GFF7014 GFF7015 - protein of unknown function DUF1486, at 31,250 to 31,630 GFF7015 GFF7016 - FAD-dependent oxidoreductase, at 31,674 to 33,164 GFF7016 Position (kb) 31 32Strain fitness (log2 ratio) -1 0 1at 30.422 kb on + strand, within GFF7014at 30.426 kb on - strand, within GFF7014at 30.426 kb on - strand, within GFF7014at 30.426 kb on - strand, within GFF7014at 30.870 kb on - strand, within GFF7014at 30.870 kb on - strand, within GFF7014at 31.175 kb on + strandat 31.175 kb on + strandat 31.628 kb on + strandat 31.629 kb on - strandat 31.629 kb on - strandat 31.629 kb on - strandat 31.794 kb on + strandat 31.836 kb on + strand, within GFF7016at 31.836 kb on + strand, within GFF7016at 32.208 kb on + strand, within GFF7016at 32.209 kb on - strand, within GFF7016at 32.355 kb on + strand, within GFF7016at 32.356 kb on - strand, within GFF7016

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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30,422 + GFF7014 0.58 -0.3
30,426 - GFF7014 0.58 +0.5
30,426 - GFF7014 0.58 +0.7
30,426 - GFF7014 0.58 +0.6
30,870 - GFF7014 0.87 -1.2
30,870 - GFF7014 0.87 -0.7
31,175 + +0.6
31,175 + +0.6
31,628 + -0.7
31,629 - -0.8
31,629 - -0.5
31,629 - +0.6
31,794 + -0.9
31,836 + GFF7016 0.11 -1.4
31,836 + GFF7016 0.11 -1.0
32,208 + GFF7016 0.36 +0.1
32,209 - GFF7016 0.36 +0.8
32,355 + GFF7016 0.46 -0.9
32,356 - GFF7016 0.46 -0.1

Or see this region's nucleotide sequence