Strain Fitness in Variovorax sp. SCN45 around GFF7005

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
20,751	+		GFF7004	0.55	+0.1
21,009	+		GFF7004	0.68	+0.5
21,010	-		GFF7004	0.68	+1.3
21,150	+		GFF7004	0.74	+0.2
21,151	-		GFF7004	0.74	-0.2
21,321	+		GFF7004	0.82	-0.4
21,366	+		GFF7004	0.84	-0.3
21,367	-		GFF7004	0.84	-0.9
21,367	-		GFF7004	0.84	-1.0
21,528	+				-0.6
21,528	+				-0.4
21,528	+				-0.6
21,529	-				+0.3
21,529	-				-1.1
21,542	+				-1.0
21,620	+				+0.0
21,950	+		GFF7005	0.24	-0.8
22,072	-		GFF7005	0.35	-0.2
22,158	-		GFF7005	0.43	-0.1
22,158	-		GFF7005	0.43	-0.1
22,244	+		GFF7005	0.51	-0.7
22,274	+		GFF7005	0.53	+0.7
22,275	-		GFF7005	0.53	-2.2
22,275	-		GFF7005	0.53	+0.4
22,275	-		GFF7005	0.53	-1.0
22,597	+		GFF7005	0.83	-0.6
22,849	+				-0.6
23,126	-		GFF7006	0.48	-0.3
23,126	-		GFF7006	0.48	-0.1
23,126	-		GFF7006	0.48	-0.1
23,188	+		GFF7006	0.56	-0.3
23,188	+		GFF7006	0.56	-0.6
23,189	-		GFF7006	0.56	-0.6
23,204	+		GFF7006	0.58	-1.0
23,205	-		GFF7006	0.59	-1.4
23,205	-		GFF7006	0.59	+0.3
23,316	+		GFF7006	0.74	+0.4
23,316	+		GFF7006	0.74	+1.6
23,317	-		GFF7006	0.74	-0.9
23,317	-		GFF7006	0.74	-1.7
23,482	+				-1.7
23,483	-				-1.6
23,541	+				+0.0
23,541	+				-2.0
23,542	-				-0.7
23,542	-				-1.0
23,542	-				-0.3
23,542	-				-0.1