Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6939 and GFF6940 are separated by 44 nucleotides GFF6940 and GFF6941 are separated by 318 nucleotides GFF6941 and GFF6942 are separated by 6 nucleotides GFF6942 and GFF6943 overlap by 4 nucleotides
GFF6939 - ABC-type antimicrobial peptide transport system, permease component, at 14,338 to 15,480
GFF6939
GFF6940 - no description, at 15,525 to 16,061
GFF6940
GFF6941 - Two-component transcriptional response regulator, LuxR family, at 16,380 to 17,249
GFF6941
GFF6942 - Universal stress protein UspA and related nucleotide-binding proteins, at 17,256 to 18,113
GFF6942
GFF6943 - no description, at 18,110 to 18,499
GFF6943
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1 at 15.425 kb on - strand at 15.425 kb on - strand at 15.706 kb on + strand, within GFF6940 at 15.706 kb on + strand, within GFF6940 at 15.706 kb on + strand, within GFF6940 at 15.857 kb on - strand, within GFF6940 at 15.857 kb on - strand, within GFF6940 at 15.964 kb on + strand, within GFF6940 at 15.964 kb on + strand, within GFF6940 at 15.964 kb on + strand, within GFF6940 at 15.965 kb on - strand, within GFF6940 at 15.965 kb on - strand, within GFF6940 at 15.965 kb on - strand, within GFF6940 at 16.285 kb on + strand at 16.673 kb on - strand, within GFF6941 at 16.990 kb on + strand, within GFF6941 at 17.201 kb on + strand at 17.202 kb on - strand at 17.248 kb on - strand at 17.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 15,425 - +0.4 15,425 - -0.3 15,706 + GFF6940 0.34 -0.1 15,706 + GFF6940 0.34 -0.3 15,706 + GFF6940 0.34 +0.3 15,857 - GFF6940 0.62 -0.7 15,857 - GFF6940 0.62 -0.0 15,964 + GFF6940 0.82 -0.5 15,964 + GFF6940 0.82 +0.4 15,964 + GFF6940 0.82 -0.1 15,965 - GFF6940 0.82 +0.0 15,965 - GFF6940 0.82 -0.3 15,965 - GFF6940 0.82 +0.5 16,285 + +0.1 16,673 - GFF6941 0.34 -0.4 16,990 + GFF6941 0.70 +0.6 17,201 + +0.4 17,202 - -0.7 17,248 - +0.6 17,248 - -0.5
Or see this region's nucleotide sequence