Strain Fitness in Variovorax sp. SCN45 around GFF6914

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6913 and GFF6914 are separated by 100 nucleotidesGFF6914 and GFF6915 are separated by 97 nucleotidesGFF6915 and GFF6916 are separated by 17 nucleotides GFF6913 - Ser-tRNA(Ala) deacylase @ Gly-tRNA(Ala) deacylase, at 49,100 to 49,873 GFF6913 GFF6914 - Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8), at 49,974 to 52,148 GFF6914 GFF6915 - Alkyl hydroperoxide reductase and/or thiol-specific antioxidant family (AhpC/TSA) protein, at 52,246 to 52,938 GFF6915 GFF6916 - Methyltransferase type 11, at 52,956 to 53,561 GFF6916 Position (kb) 49 50 51 52 53Strain fitness (log2 ratio) -1 0 1 2at 49.053 kb on + strandat 49.054 kb on - strandat 49.054 kb on - strandat 49.054 kb on - strandat 49.054 kb on - strandat 49.054 kb on - strandat 49.088 kb on + strandat 49.089 kb on - strandat 49.130 kb on + strandat 49.130 kb on + strandat 49.130 kb on + strandat 49.130 kb on + strandat 49.130 kb on + strandat 49.130 kb on + strandat 49.130 kb on + strandat 49.131 kb on - strandat 49.131 kb on - strandat 49.131 kb on - strandat 49.202 kb on + strand, within GFF6913at 49.202 kb on + strand, within GFF6913at 49.203 kb on - strand, within GFF6913at 49.203 kb on - strand, within GFF6913at 49.203 kb on - strand, within GFF6913at 49.502 kb on + strand, within GFF6913at 49.502 kb on + strand, within GFF6913at 49.503 kb on - strand, within GFF6913at 49.503 kb on - strand, within GFF6913at 49.503 kb on - strand, within GFF6913at 52.198 kb on + strandat 52.199 kb on - strandat 52.199 kb on - strandat 52.199 kb on - strandat 52.199 kb on - strandat 52.531 kb on + strand, within GFF6915at 52.532 kb on - strand, within GFF6915at 52.532 kb on - strand, within GFF6915at 52.532 kb on - strand, within GFF6915at 52.627 kb on + strand, within GFF6915at 52.627 kb on + strand, within GFF6915at 52.627 kb on + strand, within GFF6915at 52.627 kb on + strand, within GFF6915at 52.627 kb on + strand, within GFF6915at 52.628 kb on - strand, within GFF6915at 52.628 kb on - strand, within GFF6915at 52.628 kb on - strand, within GFF6915at 52.723 kb on + strand, within GFF6915at 52.723 kb on + strand, within GFF6915at 52.723 kb on + strand, within GFF6915at 52.723 kb on + strand, within GFF6915at 52.724 kb on - strand, within GFF6915at 52.724 kb on - strand, within GFF6915at 52.750 kb on + strand, within GFF6915at 52.750 kb on + strand, within GFF6915at 52.780 kb on + strand, within GFF6915at 52.780 kb on + strand, within GFF6915at 52.781 kb on - strand, within GFF6915at 52.781 kb on - strand, within GFF6915at 52.843 kb on + strand, within GFF6915at 52.843 kb on + strand, within GFF6915at 52.843 kb on + strand, within GFF6915at 52.843 kb on + strand, within GFF6915at 52.843 kb on + strand, within GFF6915at 52.843 kb on + strand, within GFF6915at 52.843 kb on + strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.844 kb on - strand, within GFF6915at 52.927 kb on + strandat 52.927 kb on + strandat 52.927 kb on + strandat 52.927 kb on + strandat 52.927 kb on + strandat 52.927 kb on + strandat 52.927 kb on + strandat 52.928 kb on - strandat 52.928 kb on - strandat 52.928 kb on - strandat 52.928 kb on - strandat 52.928 kb on - strandat 52.928 kb on - strandat 52.928 kb on - strandat 52.928 kb on - strandat 52.928 kb on - strandat 53.016 kb on + strandat 53.016 kb on + strandat 53.016 kb on + strandat 53.016 kb on + strandat 53.017 kb on - strand, within GFF6916at 53.017 kb on - strand, within GFF6916

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
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49,053 + -0.6
49,054 - -0.2
49,054 - +0.4
49,054 - +0.5
49,054 - +0.3
49,054 - -0.4
49,088 + -0.5
49,089 - -0.7
49,130 + +0.7
49,130 + -0.6
49,130 + +0.5
49,130 + +0.5
49,130 + -0.3
49,130 + -0.2
49,130 + +0.7
49,131 - +0.7
49,131 - -0.2
49,131 - -1.0
49,202 + GFF6913 0.13 -0.9
49,202 + GFF6913 0.13 +0.0
49,203 - GFF6913 0.13 -0.0
49,203 - GFF6913 0.13 -0.7
49,203 - GFF6913 0.13 -0.5
49,502 + GFF6913 0.52 -1.4
49,502 + GFF6913 0.52 -0.5
49,503 - GFF6913 0.52 +0.0
49,503 - GFF6913 0.52 -0.3
49,503 - GFF6913 0.52 -0.4
52,198 + +0.7
52,199 - +1.7
52,199 - -0.3
52,199 - +1.7
52,199 - -0.5
52,531 + GFF6915 0.41 +0.2
52,532 - GFF6915 0.41 -1.0
52,532 - GFF6915 0.41 -0.4
52,532 - GFF6915 0.41 +0.7
52,627 + GFF6915 0.55 +2.0
52,627 + GFF6915 0.55 -1.2
52,627 + GFF6915 0.55 +1.0
52,627 + GFF6915 0.55 -0.0
52,627 + GFF6915 0.55 -0.2
52,628 - GFF6915 0.55 +0.7
52,628 - GFF6915 0.55 +1.7
52,628 - GFF6915 0.55 +0.9
52,723 + GFF6915 0.69 +1.0
52,723 + GFF6915 0.69 -0.2
52,723 + GFF6915 0.69 +0.1
52,723 + GFF6915 0.69 -0.3
52,724 - GFF6915 0.69 -0.9
52,724 - GFF6915 0.69 -0.3
52,750 + GFF6915 0.73 +1.0
52,750 + GFF6915 0.73 -0.5
52,780 + GFF6915 0.77 -0.1
52,780 + GFF6915 0.77 +0.1
52,781 - GFF6915 0.77 -0.8
52,781 - GFF6915 0.77 +0.1
52,843 + GFF6915 0.86 +0.3
52,843 + GFF6915 0.86 -0.6
52,843 + GFF6915 0.86 -0.2
52,843 + GFF6915 0.86 +0.1
52,843 + GFF6915 0.86 +0.2
52,843 + GFF6915 0.86 -0.9
52,843 + GFF6915 0.86 +0.2
52,844 - GFF6915 0.86 +0.1
52,844 - GFF6915 0.86 -0.2
52,844 - GFF6915 0.86 +0.3
52,844 - GFF6915 0.86 -0.1
52,844 - GFF6915 0.86 -0.9
52,844 - GFF6915 0.86 -0.9
52,844 - GFF6915 0.86 -0.1
52,844 - GFF6915 0.86 -0.4
52,844 - GFF6915 0.86 -0.1
52,844 - GFF6915 0.86 -0.1
52,844 - GFF6915 0.86 +0.1
52,927 + +0.8
52,927 + +1.2
52,927 + -0.4
52,927 + +0.7
52,927 + +0.3
52,927 + -0.1
52,927 + -1.3
52,928 - -0.2
52,928 - +0.3
52,928 - -0.7
52,928 - +1.7
52,928 - -0.1
52,928 - +0.1
52,928 - +1.2
52,928 - -1.3
52,928 - -0.6
53,016 + -1.6
53,016 + -0.2
53,016 + -0.5
53,016 + +0.9
53,017 - GFF6916 0.10 -0.3
53,017 - GFF6916 0.10 +1.1

Or see this region's nucleotide sequence