Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6878 and GFF6879 are separated by 158 nucleotides GFF6879 and GFF6880 overlap by 4 nucleotides GFF6880 and GFF6881 are separated by 20 nucleotides GFF6881 and GFF6882 overlap by 4 nucleotides
GFF6878 - hypothetical protein, at 10,286 to 11,935
GFF6878
GFF6879 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 12,094 to 12,642
GFF6879
GFF6880 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 12,639 to 13,526
GFF6880
GFF6881 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 13,547 to 14,437
GFF6881
GFF6882 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 14,434 to 15,504
GFF6882
Position (kb)
12
13
14 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 11.685 kb on + strand, within GFF6878 at 11.685 kb on + strand, within GFF6878 at 11.685 kb on + strand, within GFF6878 at 11.685 kb on + strand, within GFF6878 at 11.685 kb on + strand, within GFF6878 at 11.685 kb on + strand, within GFF6878 at 11.685 kb on + strand, within GFF6878 at 11.686 kb on - strand, within GFF6878 at 11.686 kb on - strand, within GFF6878 at 11.699 kb on + strand, within GFF6878 at 11.742 kb on + strand, within GFF6878 at 11.742 kb on + strand, within GFF6878 at 11.742 kb on + strand, within GFF6878 at 11.743 kb on - strand, within GFF6878 at 11.743 kb on - strand, within GFF6878 at 11.743 kb on - strand, within GFF6878 at 11.743 kb on - strand, within GFF6878 at 11.744 kb on - strand, within GFF6878 at 11.793 kb on + strand at 11.793 kb on + strand at 11.794 kb on - strand at 11.794 kb on - strand at 11.874 kb on + strand at 11.874 kb on + strand at 11.874 kb on + strand at 11.874 kb on + strand at 11.874 kb on + strand at 11.874 kb on + strand at 11.874 kb on + strand at 11.875 kb on - strand at 11.875 kb on - strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.908 kb on + strand at 11.909 kb on - strand at 11.909 kb on - strand at 11.909 kb on - strand at 11.909 kb on - strand at 11.909 kb on - strand at 11.909 kb on - strand at 11.909 kb on - strand at 11.909 kb on - strand at 12.095 kb on + strand at 12.095 kb on + strand at 12.095 kb on + strand at 12.095 kb on + strand at 12.095 kb on + strand at 12.095 kb on + strand at 12.095 kb on + strand at 12.096 kb on - strand at 12.096 kb on - strand at 12.096 kb on - strand at 12.096 kb on - strand at 12.096 kb on - strand at 12.096 kb on - strand at 12.096 kb on - strand at 12.096 kb on - strand at 12.676 kb on + strand at 12.676 kb on + strand at 12.677 kb on - strand at 12.677 kb on - strand at 12.677 kb on - strand at 12.677 kb on - strand at 13.415 kb on - strand, within GFF6880 at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.526 kb on + strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.527 kb on - strand at 13.527 kb on - strand at 14.456 kb on + strand at 14.456 kb on + strand at 14.457 kb on - strand at 14.457 kb on - strand at 14.457 kb on - strand at 14.457 kb on - strand at 14.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 11,685 + GFF6878 0.85 -0.9 11,685 + GFF6878 0.85 -1.5 11,685 + GFF6878 0.85 -2.5 11,685 + GFF6878 0.85 -0.6 11,685 + GFF6878 0.85 -0.9 11,685 + GFF6878 0.85 -0.6 11,685 + GFF6878 0.85 -2.7 11,686 - GFF6878 0.85 -4.2 11,686 - GFF6878 0.85 -1.3 11,699 + GFF6878 0.86 -1.3 11,742 + GFF6878 0.88 -0.3 11,742 + GFF6878 0.88 -2.5 11,742 + GFF6878 0.88 -3.6 11,743 - GFF6878 0.88 -1.6 11,743 - GFF6878 0.88 -1.9 11,743 - GFF6878 0.88 -1.1 11,743 - GFF6878 0.88 -1.3 11,744 - GFF6878 0.88 -0.6 11,793 + -0.1 11,793 + +0.7 11,794 - -1.9 11,794 - -2.5 11,874 + -1.6 11,874 + -2.0 11,874 + -2.9 11,874 + -2.4 11,874 + -2.5 11,874 + -1.7 11,874 + -2.5 11,875 - +0.4 11,875 - -3.2 11,908 + +0.9 11,908 + -1.1 11,908 + -1.1 11,908 + -1.1 11,908 + -0.9 11,908 + -2.3 11,908 + -0.6 11,908 + -0.6 11,908 + -1.6 11,908 + -2.7 11,908 + -0.6 11,908 + -0.9 11,908 + -0.3 11,909 - -0.3 11,909 - -1.1 11,909 - -0.6 11,909 - -0.3 11,909 - -0.9 11,909 - +0.7 11,909 - -0.4 11,909 - -1.2 12,095 + +0.5 12,095 + -0.2 12,095 + +0.3 12,095 + -0.4 12,095 + -0.3 12,095 + -0.3 12,095 + -0.7 12,096 - -0.7 12,096 - +0.3 12,096 - +0.1 12,096 - -0.2 12,096 - +0.7 12,096 - -0.6 12,096 - -0.1 12,096 - +0.4 12,676 + -2.0 12,676 + -1.8 12,677 - -1.3 12,677 - -2.4 12,677 - -4.1 12,677 - +1.7 13,415 - GFF6880 0.87 -0.3 13,526 + -0.8 13,526 + +0.4 13,526 + -0.3 13,526 + +0.2 13,526 + -0.8 13,526 + +0.5 13,526 + +0.3 13,526 + +0.4 13,526 + +1.1 13,526 + +1.0 13,526 + +0.3 13,526 + -0.1 13,527 - -0.3 13,527 - +0.8 13,527 - +0.2 13,527 - -0.2 13,527 - -0.6 13,527 - -0.4 13,527 - +1.3 13,527 - -0.0 13,527 - +0.4 14,456 + -0.3 14,456 + +0.3 14,457 - -0.7 14,457 - -0.6 14,457 - -1.7 14,457 - -0.3 14,457 - +0.4
Or see this region's nucleotide sequence