Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF678 and GFF679 overlap by 8 nucleotides GFF679 and GFF680 overlap by 32 nucleotides GFF680 and GFF681 are separated by 174 nucleotides
GFF678 - Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7), at 730,047 to 733,400
GFF678
GFF679 - DNA polymerase-like protein PA0670, at 733,393 to 734,748
GFF679
GFF680 - RecA/RadA recombinase, at 734,717 to 735,523
GFF680
GFF681 - Hypothetical protein in cluster with At3g50560-like gene family, at 735,698 to 736,537
GFF681
Position (kb) 733
734
735 Strain fitness (log2 ratio) -2
-1
0
1 at 732.454 kb on + strand, within GFF678 at 732.454 kb on + strand, within GFF678 at 732.454 kb on + strand, within GFF678 at 732.454 kb on + strand, within GFF678 at 732.454 kb on + strand, within GFF678 at 732.454 kb on + strand, within GFF678 at 732.454 kb on + strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.455 kb on - strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.671 kb on + strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.672 kb on - strand, within GFF678 at 732.796 kb on + strand, within GFF678 at 732.932 kb on - strand, within GFF678 at 733.207 kb on + strand at 733.208 kb on - strand at 733.208 kb on - strand at 733.219 kb on + strand at 733.219 kb on + strand at 733.219 kb on + strand at 733.219 kb on + strand at 733.297 kb on + strand at 733.298 kb on - strand at 733.298 kb on - strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.400 kb on + strand at 733.401 kb on - strand at 733.401 kb on - strand at 733.401 kb on - strand at 733.415 kb on + strand at 733.415 kb on + strand at 733.415 kb on + strand at 733.416 kb on - strand at 733.416 kb on - strand at 733.465 kb on + strand at 733.465 kb on + strand at 733.466 kb on - strand at 733.502 kb on + strand at 733.502 kb on + strand at 733.502 kb on + strand at 733.503 kb on - strand at 733.503 kb on - strand at 733.503 kb on - strand at 733.503 kb on - strand at 733.503 kb on - strand at 733.503 kb on - strand at 733.503 kb on - strand at 733.505 kb on + strand at 733.505 kb on + strand at 733.506 kb on - strand at 733.506 kb on - strand at 733.506 kb on - strand at 733.601 kb on + strand, within GFF679 at 733.601 kb on + strand, within GFF679 at 733.662 kb on - strand, within GFF679 at 733.662 kb on - strand, within GFF679 at 733.662 kb on - strand, within GFF679 at 733.665 kb on + strand, within GFF679 at 734.646 kb on - strand at 734.723 kb on - strand at 734.723 kb on - strand at 734.723 kb on - strand at 734.723 kb on - strand at 734.723 kb on - strand at 734.723 kb on - strand at 735.255 kb on + strand, within GFF680 at 735.256 kb on - strand, within GFF680 at 735.537 kb on - strand at 735.537 kb on - strand at 735.537 kb on - strand at 735.542 kb on + strand at 735.550 kb on + strand at 735.555 kb on + strand at 735.555 kb on + strand at 735.555 kb on + strand at 735.555 kb on + strand at 735.555 kb on + strand at 735.555 kb on + strand at 735.556 kb on - strand at 735.608 kb on + strand at 735.681 kb on + strand at 735.681 kb on + strand at 735.681 kb on + strand at 735.682 kb on - strand at 735.737 kb on + strand at 735.737 kb on + strand at 735.737 kb on + strand at 735.737 kb on + strand at 735.738 kb on - strand at 735.738 kb on - strand at 735.738 kb on - strand at 735.738 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 732,454 + GFF678 0.72 -0.5 732,454 + GFF678 0.72 -0.1 732,454 + GFF678 0.72 -1.4 732,454 + GFF678 0.72 -0.7 732,454 + GFF678 0.72 +0.9 732,454 + GFF678 0.72 -1.8 732,454 + GFF678 0.72 +0.0 732,455 - GFF678 0.72 -0.1 732,455 - GFF678 0.72 -0.6 732,455 - GFF678 0.72 -0.4 732,455 - GFF678 0.72 -0.1 732,455 - GFF678 0.72 -0.4 732,455 - GFF678 0.72 +1.2 732,455 - GFF678 0.72 -0.1 732,455 - GFF678 0.72 +0.0 732,455 - GFF678 0.72 +0.1 732,455 - GFF678 0.72 +0.4 732,671 + GFF678 0.78 -0.9 732,671 + GFF678 0.78 -0.4 732,671 + GFF678 0.78 +0.5 732,671 + GFF678 0.78 -1.1 732,671 + GFF678 0.78 +0.0 732,671 + GFF678 0.78 +0.0 732,671 + GFF678 0.78 -0.1 732,671 + GFF678 0.78 -0.2 732,671 + GFF678 0.78 +0.6 732,671 + GFF678 0.78 -0.6 732,671 + GFF678 0.78 -0.4 732,671 + GFF678 0.78 +0.3 732,672 - GFF678 0.78 +0.0 732,672 - GFF678 0.78 -0.5 732,672 - GFF678 0.78 +0.3 732,672 - GFF678 0.78 +0.7 732,672 - GFF678 0.78 -1.4 732,672 - GFF678 0.78 +0.2 732,672 - GFF678 0.78 +0.3 732,672 - GFF678 0.78 -0.5 732,672 - GFF678 0.78 -1.6 732,672 - GFF678 0.78 -0.8 732,672 - GFF678 0.78 -0.3 732,672 - GFF678 0.78 +1.2 732,796 + GFF678 0.82 +0.4 732,932 - GFF678 0.86 +0.5 733,207 + -0.1 733,208 - -0.2 733,208 - -0.1 733,219 + -0.2 733,219 + +0.5 733,219 + +0.3 733,219 + +0.1 733,297 + -0.1 733,298 - +0.4 733,298 - -1.0 733,400 + -0.5 733,400 + +0.0 733,400 + -0.1 733,400 + -0.0 733,400 + -0.5 733,400 + -0.7 733,400 + -0.4 733,400 + -0.1 733,400 + -0.0 733,401 - -0.3 733,401 - -0.3 733,401 - -1.0 733,415 + -0.2 733,415 + -1.0 733,415 + -0.3 733,416 - -1.4 733,416 - +0.0 733,465 + +0.8 733,465 + -0.4 733,466 - -0.7 733,502 + +0.0 733,502 + +0.2 733,502 + -0.7 733,503 - -1.0 733,503 - -0.6 733,503 - -0.2 733,503 - -0.3 733,503 - +0.5 733,503 - -0.1 733,503 - -0.4 733,505 + -1.1 733,505 + +0.6 733,506 - -1.6 733,506 - -0.8 733,506 - -0.4 733,601 + GFF679 0.15 -2.3 733,601 + GFF679 0.15 -1.9 733,662 - GFF679 0.20 -0.3 733,662 - GFF679 0.20 +0.3 733,662 - GFF679 0.20 -0.3 733,665 + GFF679 0.20 -1.4 734,646 - -0.1 734,723 - -1.8 734,723 - +0.2 734,723 - +0.4 734,723 - -0.2 734,723 - +0.9 734,723 - -0.1 735,255 + GFF680 0.67 +0.1 735,256 - GFF680 0.67 -1.7 735,537 - -0.1 735,537 - +0.3 735,537 - -1.1 735,542 + +0.7 735,550 + +0.8 735,555 + -0.9 735,555 + +0.3 735,555 + -0.2 735,555 + -0.1 735,555 + +1.7 735,555 + -0.1 735,556 - +0.2 735,608 + -0.8 735,681 + +0.3 735,681 + -1.8 735,681 + +0.1 735,682 - +0.0 735,737 + -0.4 735,737 + -0.0 735,737 + +0.3 735,737 + +0.7 735,738 - -0.8 735,738 - +0.2 735,738 - +0.1 735,738 - -2.5
Or see this region's nucleotide sequence