Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF672 and GFF673 are separated by 114 nucleotides GFF673 and GFF674 are separated by 78 nucleotides GFF674 and GFF675 are separated by 10 nucleotides GFF675 and GFF676 are separated by 33 nucleotides
GFF672 - Transcriptional regulator, LysR family, at 724,844 to 725,752
GFF672
GFF673 - Lysophospholipase L1 and related esterases, at 725,867 to 727,138
GFF673
GFF674 - no description, at 727,217 to 727,438
GFF674
GFF675 - KEGG: 2'-5' RNA ligase , at 727,449 to 727,976
GFF675
GFF676 - Aminoglycoside 9-phosphotransferase (EC 2.7.1.-) => APH(9)-I, at 728,010 to 729,026
GFF676
Position (kb)
725
726
727
728 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 724.947 kb on + strand, within GFF672 at 724.947 kb on + strand, within GFF672 at 724.947 kb on + strand, within GFF672 at 724.948 kb on - strand, within GFF672 at 724.948 kb on - strand, within GFF672 at 724.948 kb on - strand, within GFF672 at 724.948 kb on - strand, within GFF672 at 725.106 kb on + strand, within GFF672 at 725.178 kb on + strand, within GFF672 at 725.187 kb on + strand, within GFF672 at 725.187 kb on + strand, within GFF672 at 725.188 kb on - strand, within GFF672 at 725.188 kb on - strand, within GFF672 at 725.241 kb on + strand, within GFF672 at 725.241 kb on + strand, within GFF672 at 725.241 kb on + strand, within GFF672 at 725.241 kb on + strand, within GFF672 at 725.242 kb on - strand, within GFF672 at 725.242 kb on - strand, within GFF672 at 725.242 kb on - strand, within GFF672 at 725.242 kb on - strand, within GFF672 at 725.400 kb on + strand, within GFF672 at 725.401 kb on - strand, within GFF672 at 725.526 kb on + strand, within GFF672 at 725.526 kb on + strand, within GFF672 at 725.526 kb on + strand, within GFF672 at 725.527 kb on - strand, within GFF672 at 725.761 kb on + strand at 725.761 kb on + strand at 725.762 kb on - strand at 725.762 kb on - strand at 725.762 kb on - strand at 725.801 kb on - strand at 725.812 kb on + strand at 725.813 kb on - strand at 725.813 kb on - strand at 725.813 kb on - strand at 725.813 kb on - strand at 726.320 kb on + strand, within GFF673 at 726.321 kb on - strand, within GFF673 at 726.377 kb on + strand, within GFF673 at 726.377 kb on + strand, within GFF673 at 726.377 kb on + strand, within GFF673 at 726.449 kb on + strand, within GFF673 at 726.449 kb on + strand, within GFF673 at 726.449 kb on + strand, within GFF673 at 726.449 kb on + strand, within GFF673 at 726.449 kb on + strand, within GFF673 at 726.450 kb on - strand, within GFF673 at 726.487 kb on + strand, within GFF673 at 726.488 kb on - strand, within GFF673 at 726.488 kb on - strand, within GFF673 at 726.490 kb on + strand, within GFF673 at 726.727 kb on + strand, within GFF673 at 726.911 kb on + strand, within GFF673 at 726.911 kb on + strand, within GFF673 at 726.911 kb on + strand, within GFF673 at 726.911 kb on + strand, within GFF673 at 726.911 kb on + strand, within GFF673 at 726.911 kb on + strand, within GFF673 at 726.912 kb on - strand, within GFF673 at 726.912 kb on - strand, within GFF673 at 727.082 kb on + strand at 727.082 kb on + strand at 727.083 kb on - strand at 727.083 kb on - strand at 727.083 kb on - strand at 727.148 kb on + strand at 727.149 kb on - strand at 727.149 kb on - strand at 727.149 kb on - strand at 727.151 kb on - strand at 727.155 kb on + strand at 727.155 kb on + strand at 727.156 kb on - strand at 727.159 kb on + strand at 727.159 kb on + strand at 727.159 kb on + strand at 727.160 kb on - strand at 727.160 kb on - strand at 727.160 kb on - strand at 727.160 kb on - strand at 727.160 kb on - strand at 727.160 kb on - strand at 727.163 kb on + strand at 727.163 kb on + strand at 727.164 kb on - strand at 727.164 kb on - strand at 727.164 kb on - strand at 727.168 kb on + strand at 727.169 kb on - strand at 727.356 kb on + strand, within GFF674 at 727.487 kb on + strand at 727.590 kb on + strand, within GFF675 at 727.590 kb on + strand, within GFF675 at 727.590 kb on + strand, within GFF675 at 727.591 kb on - strand, within GFF675 at 727.591 kb on - strand, within GFF675 at 727.962 kb on + strand at 727.962 kb on + strand at 727.998 kb on + strand at 727.999 kb on - strand at 727.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 2 mg/mL remove 724,947 + GFF672 0.11 -0.7 724,947 + GFF672 0.11 -0.9 724,947 + GFF672 0.11 +0.3 724,948 - GFF672 0.11 +0.5 724,948 - GFF672 0.11 +0.0 724,948 - GFF672 0.11 -1.4 724,948 - GFF672 0.11 -1.2 725,106 + GFF672 0.29 -0.7 725,178 + GFF672 0.37 -1.1 725,187 + GFF672 0.38 -0.6 725,187 + GFF672 0.38 -0.2 725,188 - GFF672 0.38 -1.4 725,188 - GFF672 0.38 -0.8 725,241 + GFF672 0.44 -0.0 725,241 + GFF672 0.44 -0.2 725,241 + GFF672 0.44 -0.4 725,241 + GFF672 0.44 -1.4 725,242 - GFF672 0.44 +0.3 725,242 - GFF672 0.44 -0.5 725,242 - GFF672 0.44 +0.1 725,242 - GFF672 0.44 -0.6 725,400 + GFF672 0.61 +0.2 725,401 - GFF672 0.61 -0.0 725,526 + GFF672 0.75 -0.3 725,526 + GFF672 0.75 -0.3 725,526 + GFF672 0.75 -1.1 725,527 - GFF672 0.75 +0.6 725,761 + -0.1 725,761 + +2.2 725,762 - -1.8 725,762 - -0.3 725,762 - +1.2 725,801 - +0.1 725,812 + -1.5 725,813 - -0.4 725,813 - +0.6 725,813 - -0.7 725,813 - -0.2 726,320 + GFF673 0.36 +0.2 726,321 - GFF673 0.36 -0.0 726,377 + GFF673 0.40 +0.0 726,377 + GFF673 0.40 -1.0 726,377 + GFF673 0.40 +0.1 726,449 + GFF673 0.46 +0.1 726,449 + GFF673 0.46 +0.2 726,449 + GFF673 0.46 +0.3 726,449 + GFF673 0.46 +1.2 726,449 + GFF673 0.46 -0.0 726,450 - GFF673 0.46 -0.3 726,487 + GFF673 0.49 +1.2 726,488 - GFF673 0.49 -0.3 726,488 - GFF673 0.49 -0.8 726,490 + GFF673 0.49 -0.7 726,727 + GFF673 0.68 -0.5 726,911 + GFF673 0.82 -0.1 726,911 + GFF673 0.82 +0.5 726,911 + GFF673 0.82 -1.1 726,911 + GFF673 0.82 +0.8 726,911 + GFF673 0.82 -0.2 726,911 + GFF673 0.82 +0.5 726,912 - GFF673 0.82 +1.9 726,912 - GFF673 0.82 -1.6 727,082 + -0.9 727,082 + +0.8 727,083 - -1.0 727,083 - -0.4 727,083 - +0.2 727,148 + -1.1 727,149 - -0.1 727,149 - +1.2 727,149 - -0.2 727,151 - -0.4 727,155 + +1.5 727,155 + +0.3 727,156 - +0.2 727,159 + -0.6 727,159 + -0.0 727,159 + -0.4 727,160 - -0.9 727,160 - +0.5 727,160 - +0.6 727,160 - -0.5 727,160 - +0.8 727,160 - +0.2 727,163 + -0.5 727,163 + -0.2 727,164 - -0.8 727,164 - -0.4 727,164 - -0.3 727,168 + -0.4 727,169 - +0.8 727,356 + GFF674 0.63 -0.6 727,487 + -1.1 727,590 + GFF675 0.27 -0.1 727,590 + GFF675 0.27 -0.9 727,590 + GFF675 0.27 +0.0 727,591 - GFF675 0.27 -1.8 727,591 - GFF675 0.27 -0.3 727,962 + +0.1 727,962 + +0.1 727,998 + +1.0 727,999 - -0.7 727,999 - +0.8
Or see this region's nucleotide sequence